Preparation and biodistribution of [67Ga]-DTPA-rituximab in normal rats

Chelating agents for radiolanthanides: Applications to imaging and therapy

“…124 A more rapid and reliable radiolabelling method with 177 Lu has been subsequently reported. 125 DTPA conjugates with rituximab are also well known and have been radiolabelled with a range of radionuclides including 99m Tc 126 and 67 Ga. 127 Research on the 177 Lu-CHX-A''-DTPArituximab is well developed and animal studies have shown promising tumour uptake and targeting. 128 153 Sm-DTPA-rituximab has also been reported and was used to obtain SPECT images on rats 129 and Beyer et al have described the synthesis and use of 149 Tb-CHX-A"-DTPA complexes in the formation of conjugates with the antibody rituximab.…”

Development of 67Ga-labeled streptokinase

“…In order to obtain an STP conjugate for use in diagnostic studies using metallic positron emission tomography (PET) or single photon emission tomography (SPECT) radioisotopes, 67 Ga-labeled STP was prepared for preliminary biodistribution studies, based on our recent experiences in the preparation of gallium-labeled proteins [10]. Radiolabeled enzyme was injected intravenously to normal and thrombotic rat tail vein, and the biodistribution of the tracer was checked among the tissues using scarification and SPECT.…”

“…Radiolabeling of the enzyme conjugate with 67 Ga: The protein DTPA-conjugate was labeled using an optimization protocol according to the literature [10,11]. Typically, 37 -40 MBq of 67 Ga-chloride (in 0.2 M HCl) was added to a conical vial and dried under a flow of nitrogen.…”

Preparation and evaluation of [67Ga]-DTPA-β-1–24-corticotrophin in normal rats

“…In continuation of our recent works on the labeling of proteins and antibodies with cyclotron-derived radioisotopes [11][12][13], we were interested in radiolabeling of biologically active peptides.…”