Preparation and application of aptamer-functionalized sorbent for the analysis of ultra-trace aflatoxin M1 and analogues in milk

Zhang, Yi; Huang, Yuanxiang; Yue, Zhenfeng; Fan, Huajun; Wu, Shaojing

doi:10.1016/j.microc.2021.106179

Cited by 14 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After optimizing the concentrations of the cations in the solution, the supporting electrolyte, Tris/ HCl 50 mmol L À 1 , MgCl 2 10 mmol L À 1 , and NaCl 10 mmol L À 1 , was defined for further studies. An optimal concentration of MgCl 2 10 mmol L À 1 in the solution medium for efficient binding of aptamer with aflatoxin M1 has been reported previously [38].…”

Section: Effect Of Support Electrolyte Salt Concentrationmentioning

confidence: 88%

A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs

2023

View full text Add to dashboard Cite

Cortisol is a steroid hormone naturally produced by the adrenal glands. It participates in and controls several processes in the body and is considered an important physiological biomarker. Due to its very low concentrations in body fluids, its detection requires high sensitivity and specificity. Here, we present a simple electrochemical biosensor based on reduced graphene oxide (rGO) modified with gold nanoparticles (AuNPs) for the immobilization of the cortisol‐specific aptamer (Ap) (MCH‐Ap‐AurGO/GCE). Important analytical parameters for identifying the target analyte were optimized, such as conditions and amount of immobilized Ap, the influence of the concentration and nature of the supporting electrolyte, pH of the medium, and incubation time. The optimized conditions for the aptasensor were: concentration of Ap 1.0 × 10−6 mol L−1, support electrolyte Tris/HCl 50 mmol L−1, MgCl2 10 mmol L−1, and NaCl 10 mmol L−1, at pH 5.0 and incubation time of 15 min. A linear response range was obtained from 1 × 10−18 up to 1 × 10−11 mol L−1 of cortisol with a detection limit (LOD) of 1.0 × 10−18 mol L−1. A curve adjusted for operational purposes in the saliva sample was fitted for the concentration range between 0.5 × 10−14 and 1 × 10−11 mol L−1, with a linear regression equation ΔRtc/Rtc1 = 2.70 + 0.17 × log([Cortisol]). The aptasensor demonstrated a great potential for detecting cortisol in a simple, fast, and highly sensitive way, opening its path for application in real samples, which present levels below the concentration in which cortisol is commonly found in body fluids.

show abstract

Section: Effect Of Support Electrolyte Salt Concentrationmentioning

confidence: 88%

A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs

2023

View full text Add to dashboard Cite

show abstract

“…With the emergence of new technologies and novel materials, sample pretreatment methods are also developing. Table S2 [ 11 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , …”

Section: Sample Pretreatment Methodsmentioning

confidence: 99%

“…Recently, based on the specific recognition ability of “chemical antibody”, a new type of SPE adsorbent was developed by using aptamer modified carrier surface as functional monomer, which has advantages of specificity, accuracy, and repeatability for enriching ultra-trace targets. Zhang et al [ 70 ] prepared and applied an M1-aptamer-functionalized sorbent for the adsorption of ultra-trace AFM1 and analogues in milk samples, as shown in Figure 2 A. During the extraction process, the M1-aptamer on the surface of the carboxyl microspheres were completely modified via N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC)/NHs amide formation.…”

Section: Sample Pretreatment Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Recent Insights into Sample Pretreatment Methods for Mycotoxins in Different Food Matrices: A Critical Review on Novel Materials

Zhang

Zhou

et al. 2023

Toxins

View full text Add to dashboard Cite

Mycotoxins pollution is a global concern, and can pose a serious threat to human health. People and livestock eating contaminated food will encounter acute and chronic poisoning symptoms, such as carcinogenicity, acute hepatitis, and a weakened immune system. In order to prevent or reduce the exposure of human beings and livestock to mycotoxins, it is necessary to screen mycotoxins in different foods efficiently, sensitively, and selectively. Proper sample preparation is very important for the separation, purification, and enrichment of mycotoxins from complex matrices. This review provides a comprehensive summary of mycotoxins pretreatment methods since 2017, including traditionally used methods, solid-phase extraction (SPE)-based methods, liquid-liquid extraction (LLE)-based methods, matrix solid phase dispersion (MSPD), QuEChERS, and so on. The novel materials and cutting-edge technologies are systematically and comprehensively summarized. Moreover, we discuss and compare the pros and cons of different pretreatment methods and suggest a prospect.

show abstract

“…AFM1 can be found in commercially available milk and is generally considered as one of the most serious problems for food safety [ 31 ]. Therefore, its concentration in milk is strictly regulated and the maximum permissible levels of AFM1 in milk and dairy products are set at 0.05 μg kg −1 in the European Union [ 232 ]. An impedimetric aptasensor for the determination of AFM1 in milk, employing the aptamer immobilization at a carboxyphenyl layer grafted by diazonium chemistry onto the surfaces of SPCEs, was developed by Istamboulié et al [ 74 ].…”

Section: Electrochemical Aptasensors Developed Using Aryldiazonium Chemistry For Food Safety Monitoring Applicationsmentioning

confidence: 99%

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

View full text Add to dashboard Cite

Food safety monitoring assays based on synthetic recognition structures such as aptamers are receiving considerable attention due to their remarkable advantages in terms of their ability to bind to a wide range of target analytes, strong binding affinity, facile manufacturing, and cost-effectiveness. Although aptasensors for food monitoring are still in the development stage, the use of an electrochemical detection route, combined with the wide range of materials available as transducers and the proper immobilization strategy of the aptamer at the transducer surface, can lead to powerful analytical tools. In such a context, employing aryldiazonium salts for the surface derivatization of transducer electrodes serves as a simple, versatile and robust strategy to fine-tune the interface properties and to facilitate the convenient anchoring and stability of the aptamer. By summarizing the most important results disclosed in the last years, this article provides a comprehensive review that emphasizes the contribution of aryldiazonium chemistry in developing electrochemical aptasensors for food safety monitoring.

show abstract

Preparation and application of aptamer-functionalized sorbent for the analysis of ultra-trace aflatoxin M1 and analogues in milk

Cited by 14 publications

References 36 publications

A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs

A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs

Recent Insights into Sample Pretreatment Methods for Mycotoxins in Different Food Matrices: A Critical Review on Novel Materials

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Contact Info

Product

Resources

About