Preparation and antitumor activity of bFGF-mediated active targeting doxorubicin microbubbles

Abstract

Characterization and antitumor activity of basic fibroblast growth factor-mediated active targeting doxorubicin microbubbles (bFGF-DOX-MB) were investigated. Pluronic F68 with chemical conjugation of doxorubicin (DOX-P) and peptide KRTGQYKLC-conjugated DSPE-PEG2000 were prepared. bFGF-DOX-MB had a normal distribution of particle size, with average particle size of 2.7 μm. Using A549 mouse model, bFGF-DOX-MB combined ultrasound showed the best inhibition effect on tumor volume growth among all the test groups. Similar conclusion was obtained from experimental measurements of tumor weight change and blood cell count. From the results, chemotherapeutic drug inhibition on tumor growth could be enhanced by local ultrasound combined with active targeting bFGF-DOX-MB, which might provide a potential application for ultrasound-mediated chemotherapy.

Development of a prototype of a theranostic system based on silica nanoparticles with immobilized fluorescent dyes and VEGF targeting ligand.

“…Достаточная изученность функции рецепторов и кристаллической структуры аффинно-связанных пар лиганд/рецептор делает их пригодными для молекулярного моделирования, описывающего массивные конформационные и структурные изменения, происходящие в момент образования пары. Например, перспективными кандидатами для разработки систем лиганд/рецептор-опосредованной доставки можно считать пары VEGF/VEGFR [25], EGF/ EGFR [26, 27] и FGF/FGFR [28,29].…”

State-of-the-art materials for ultrasound-triggered drug delivery

“…Instead of relying on electrostatic uptake of DOX in the microbubble shell, Wu et al . [85] chemically combined DOX to pluronic F68 triblock polymers, a process described earlier by Zhao et al . [86], and formulated Tween-based microbubbles along with a lipid-conjugated bFGF targeting peptide, which were developed by Terada et al .…”

Photo- and Sono-Dynamic Therapy: A Review of Mechanisms and Considerations for Pharmacological Agents Used in Therapy Incorporating Light and Sound

“…In 2013, Wu et al chemically conjugated DOX with Pluronic F68 and synthesized targeted DOX-loaded microbubbles with bFGF. Through tumor volume measurement, targeted DOX-loaded microbubbles significantly inhibited tumor growth with minor side effects in comparison with either free DOX or free DOX-Pluronic [15]. Despite the widely accepted concept that the drug molecules enter cell membrane through pores created by sonoporation, De Cock et al proposed that rather than passive diffusion and endocytosis, the direct deposition of nanoparticles from a local bubble to the cell membrane under the influence of ultrasound (termed as 'sonoprinting') is more likely to be the mechanism of large molecule uptake [122].…”

“…Alongside PDTderivated chemical-based SDT, ultrasound itself can also induce cell death in localized areas by mechanical stress and thermal effects with the help of ultrasound-sensitive substances, many of which could be modified with targeting molecules. Such therapeutic characteristics of ultrasound can enable synergic effects, providing the possibility of combined therapy [14,15]. Up to date reviews concerning PDT and SDT mostly focus on single source irradiation, that is, to use only light or ultrasound to trigger therapy.…”