Preparation and antibiofilm studies of curcumin loaded chitosan nanoparticles against polymicrobial biofilms of Candida albicans and Staphylococcus aureus

Ma, Su; Moser, Doris; Han, Feng; Leonhard, Matthias; Schneider‐Stickler, Berit; Tan, Yulong

doi:10.1016/j.carbpol.2020.116254

Cited by 71 publications

(33 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, this was the case, and our results demonstrated a potent dose-response activity of AgNPs against preformed mixed C. albicans /MRSA biofilms. Interestingly, to our knowledge, there is only one additional recent report in the published literature on the use of nanotechnological approaches, more specifically curcumin loaded chitosan nanoparticles, against polymicrobial biofilms formed by these two microorganisms [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Mixed Biofilms of Candida albicans and Methicillin-Resistant Staphylococcus aureus by Positively Charged Silver Nanoparticles and Functionalized Silicone Elastomers

Lara

Lopez-Ribot

2020

Pathogens

View full text Add to dashboard Cite

Both bacterial and fungal organisms display the ability to form biofilms; however, mixed bacterial/fungal biofilms are particularly difficult to control and eradicate. The opportunistic microbial pathogens Candida albicans and Staphylococcus aureus are among the most frequent causative agents of healthcare-acquired infections, and are often co-isolated forming mixed biofilms, especially from contaminated catheters. These mixed species biofilms display a high level of antibiotic resistance; thus, these infections are challenging to treat resulting in excess morbidity and mortality. In the absence of effective conventional antibiotic treatments, nanotechnology-based approaches represent a promising alternative for the treatment of highly recalcitrant polymicrobial biofilm infections. Our group has previously reported on the activity of pure positively charged silver nanoparticles synthesized by a novel microwave technique against single-species biofilms of C. albicans and S. aureus. Here, we have expanded our observations to demonstrate that that silver nanoparticles display dose-dependent activity against dual-species C. albicans/S. aureus biofilms. Moreover, the same nanoparticles were used to functionalize catheter materials, leading to the effective inhibition of the mixed fungal/bacterial biofilms. Overall, our results indicate the potent activity of silver nanoparticles against these cross-kingdom biofilms. More studies are warranted to examine the ability of functionalized catheters in the prevention of catheter-related bloodstream infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of Mixed Biofilms of Candida albicans and Methicillin-Resistant Staphylococcus aureus by Positively Charged Silver Nanoparticles and Functionalized Silicone Elastomers

Lara

Lopez-Ribot

2020

Pathogens

View full text Add to dashboard Cite

show abstract

“…Curcumin was able to reduce mixed biofilm formation by >85% and reduced A. baumannii virulence in a C. elegans model without exhibiting toxicity; however, the in vivo activity against mixed biofilms was not assessed. Unfortunately, the therapeutic application of curcumin is hindered due to its instability and low bioavailability [124].…”

Section: Plant-derived Componentsmentioning

confidence: 99%

“…Several studies reported the use of NPs as drug delivery systems of natural compounds with antimicrobial properties. Taking advantage of the antimicrobial activity of curcumin, curcumin-loaded chitosan NPs were developed and tested against S. aureus and C. albicans mono-and polymicrobial biofilms [124]. The ability of these NPs to penetrate biofilms was evaluated, and NPs were observed both at the surface and deeper regions of the biofilm, which is likely a consequence of the NPs' positive charge, promoting its interaction with negatively-charged biofilm components and microbial cells.…”

Section: Nanoparticlesmentioning

confidence: 99%

“…The ability of these NPs to penetrate biofilms was evaluated, and NPs were observed both at the surface and deeper regions of the biofilm, which is likely a consequence of the NPs' positive charge, promoting its interaction with negatively-charged biofilm components and microbial cells. Thus, the low diffusion of free curcumin into the biofilm is overcome by encapsulation into NPs [124]. Curcumin-loaded NPs were more effective against preformed biofilms compared to free curcumin, with a biofilm reduction of 84.36%.…”

Section: Nanoparticlesmentioning

confidence: 99%

See 1 more Smart Citation

Microbial Interkingdom Biofilms and the Quest for Novel Therapeutic Strategies

et al. 2021

View full text Add to dashboard Cite

Fungal and bacterial species interact with each other within polymicrobial biofilm communities in various niches of the human body. Interactions between these species can greatly affect human health and disease. Diseases caused by polymicrobial biofilms pose a major challenge in clinical settings because of their enhanced virulence and increased drug tolerance. Therefore, different approaches are being explored to treat fungal–bacterial biofilm infections. This review focuses on the main mechanisms involved in polymicrobial drug tolerance and the implications of the polymicrobial nature for the therapeutic treatment by highlighting clinically relevant fungal–bacterial interactions. Furthermore, innovative treatment strategies which specifically target polymicrobial biofilms are discussed.

show abstract

“…Variation of PS dosage and light intensity can be exploited to control the extent of photodynamic effect; however, accomplishing selective antimicrobial activity is still a challenge due to the concentration-dependent aggregation of PS molecules [ 9 ], which can lead to limited ROS generation [ 10 ], water solubility [ 6 , 11 ] and visible light absorption [ 12 ]. These limitations restrict the applicability of PDT in the clinical management of infectious diseases [ 13 ], at a time when the need for new antimicrobial strategies has never been more critical.…”

Section: Introductionmentioning

confidence: 99%

Hydrolytic Degradability, Cell Tolerance and On-Demand Antibacterial Effect of Electrospun Photodynamically Active Fibres

et al. 2020

View full text Add to dashboard Cite

Photodynamically active fibres (PAFs) are a novel class of stimulus-sensitive systems capable of triggering antibiotic-free antibacterial effect on-demand when exposed to light. Despite their relevance in infection control, however, the broad clinical applicability of PAFs has not yet been fully realised due to the limited control in fibrous microstructure, cell tolerance and antibacterial activity in the physiologic environment. We addressed this challenge by creating semicrystalline electrospun fibres with varying content of poly[(l-lactide)-co-(glycolide)] (PLGA), poly(ε-caprolactone) (PCL) and methylene blue (MB), whereby the effect of polymer morphology, fibre composition and photosensitiser (PS) uptake on wet state fibre behaviour and functions was studied. The presence of crystalline domains and PS–polymer secondary interactions proved key to accomplishing long-lasting fibrous microstructure, controlled mass loss and controlled MB release profiles (37 °C, pH 7.4, 8 weeks). PAFs with equivalent PLGA:PCL weight ratio successfully promoted attachment and proliferation of L929 cells over a 7-day culture with and without light activation, while triggering up to 2.5 and 4 log reduction in E. coli and S. mutans viability, respectively. These results support the therapeutic applicability of PAFs for frequently encountered bacterial infections, opening up new opportunities in photodynamic fibrous systems with integrated wound healing and infection control capabilities.

show abstract

Preparation and antibiofilm studies of curcumin loaded chitosan nanoparticles against polymicrobial biofilms of Candida albicans and Staphylococcus aureus

Cited by 71 publications

References 46 publications

Inhibition of Mixed Biofilms of Candida albicans and Methicillin-Resistant Staphylococcus aureus by Positively Charged Silver Nanoparticles and Functionalized Silicone Elastomers

Inhibition of Mixed Biofilms of Candida albicans and Methicillin-Resistant Staphylococcus aureus by Positively Charged Silver Nanoparticles and Functionalized Silicone Elastomers

Microbial Interkingdom Biofilms and the Quest for Novel Therapeutic Strategies

Hydrolytic Degradability, Cell Tolerance and On-Demand Antibacterial Effect of Electrospun Photodynamically Active Fibres

Contact Info

Product

Resources

About