Preparation and Antibacterial Properties of Silver-Doped Nanoscale Hydroxyapatite Pastes for Bone Repair and Augmentation

Wilcock, Caroline J.; Stafford, Graham P.; Miller, Cheryl A.; Ryabenkova, Yulia; Fatima, Mehreen; Gentile, Piergiorgio; Möbus, Günter; Hatton, Paul V.

doi:10.1166/jbn.2017.2387

Cited by 27 publications

(26 citation statements)

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“…We were able to go one step further by confirming via TEM XRD that the denser (black) areas on the TEM contained silver. This had been hypothesized by the previous paper but not directly confirmed [21]. Under XRD we confirmed that the material was both hydroxyapatite and that it contained silver.…”

Section: Discussionsupporting

confidence: 44%

“…Here we present the fabrication of novel, dual-function scaffolds that combine silver to impart antimicrobial properties and hydroxyapatite to enhance bone regeneration. Our laboratory previously developed a silver-doped nano-hydroxyapatite material formed by the wet precipitation method [21]. Here we show the incorporation of this innovative material into an electrospun scaffold alongside a thorough investigation of metal ion release, antimicrobial activity against clinically relevant bacterial strains, and in vitro biocompatibility using cultured mammalian MSCs.…”

Section: Introductionmentioning

confidence: 86%

“…This was based on our previously published methods [10,21]. In brief, calcium hydroxide (Sigma Aldrich, Dorset, UK) (50 mmol) was suspended in 500 mL deionized water (dH 2 O) into which 0, 1, 2.5, or 5 mmol silver nitrate (Sigma Aldrich, UK) (corresponding to 0, 2, 5, or 10 mol%, respectively) was added and stirred at 400 rpm for 1 h on a hotplate (Fisher Scientific, Loughborough, UK) at 90 • C. Phosphoric acid (Sigma Aldrich, UK) (30 mmol) was dissolved in 250 mL dH 2 O, poured into the calcium and silver preparation, and stirred for a further hour.…”

Section: Silver Nano-hydroxyapatite (Ca 10 (Po 4 ) 6 (Oh) 2 ) Manufacmentioning

confidence: 99%

“…Saravanan et al [20] produced scaffolds using the freeze-drying technique incorporating nano-hydroxyapatite with nanoparticles of silver. Wilcock et al [21] developed a novel, rapid mixing, wet precipitation method of producing bioinspired, nanoscale hydroxyapatite crystals doped with silver for antimicrobial action. A degradable scaffold could be placed against existing implants or used to hold packed bone substitutes (e.g., bone chips) into a bone defect.…”

Section: Introductionmentioning

confidence: 99%

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Electrospun Scaffolds Containing Silver-Doped Hydroxyapatite with Antimicrobial Properties for Applications in Orthopedic and Dental Bone Surgery

Paterson

Shi

Tian

et al. 2020

JFB

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Preventing the development of osteomyelitis while enhancing bone regeneration is challenging, with relatively little progress to date in translating promising technologies to the clinic. Nanoscale hydroxyapatite (nHA) has been employed as a bone graft substitute, and recent work has shown that it may be modified with silver to introduce antimicrobial activity against known pathogens. The aim of this study was to incorporate silver-doped nHA into electrospun scaffolds for applications in bone repair. Silver-doped nHA was produced using a modified, rapid mixing, wet precipitation method at 2, 5, 10 mol.% silver. The silver-doped nHA was added at 20 wt.% to a polycaprolactone solution for electrospinning. Bacteria studies demonstrated reduced bacterial presence, with Escherichia coli and Staphylococcus aureus undetectable after 96 h of exposure. Mesenchymal stem cells (MSCs) were used to study both toxicity and osteogenicity of the scaffolds using PrestoBlue® and alkaline phosphatase (ALP) assays. Innovative silver nHA scaffolds significantly reduced E. coli and S. aureus bacterial populations while maintaining cytocompatibility with mammalian cells and enhancing the differentiation of MSCs into osteoblasts. It was concluded that silver-doped nHA containing scaffolds have the potential to act as an antimicrobial device while supporting bone tissue healing for applications in orthopedic and dental bone surgery.

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Section: Discussionsupporting

confidence: 44%

Section: Introductionmentioning

confidence: 86%

Section: Silver Nano-hydroxyapatite (Ca 10 (Po 4 ) 6 (Oh) 2 ) Manufacmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electrospun Scaffolds Containing Silver-Doped Hydroxyapatite with Antimicrobial Properties for Applications in Orthopedic and Dental Bone Surgery

Paterson

Shi

Tian

et al. 2020

JFB

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show abstract

“…These materials in the form of paste have been extensively used as the bone cements in orthopedic surgery, which can be molded or injected into bone defects and implants sites, followed by in situ setting and hardening [ 3 ]. In addition, antibacterial agents (e.g., silver) can be incorporated into CaP pastes, which can be effective for the prevention and treatment of infections [ 4 ]. In particular, when formulated into porous structure, porous CaP scaffolds can provide a favorable environment for cellular reaction and colonization by osteoblasts, thus stimulating bone ingrowth into pores and bone regeneration in osseous sites [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Coextrusion-Based 3D Plotting of Ceramic Pastes for Porous Calcium Phosphate Scaffolds Comprised of Hollow Filaments

Koh

Kim

2018

Materials

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This paper demonstrates the utility of coextrusion-based 3D plotting of ceramic pastes (CoEx-3DP) as a new type of additive manufacturing (AM) technique, which can produce porous calcium phosphate (CaP) ceramic scaffolds comprised of hollow CaP filaments. In this technique, green filaments with a controlled core/shell structure can be produced by coextruding an initial feedrod, comprised of the carbon black (CB) core and CaP shell, through a fine nozzle in an acetone bath and then deposited in a controlled manner according to predetermined paths. In addition, channels in CaP filaments can be created through the removal of the CB cores during heat-treatment. Produced CaP scaffolds had two different types of pores with well-defined geometries: three-dimensionally interconnected pores (~360 × 230 μm2 in sizes) and channels (>100 μm in diameter) in hollow CaP filaments. The porous scaffolds showed high compressive strengths of ~12.3 ± 2.2 MPa at a high porosity of ~73 vol % when compressed parallel to the direction of the hollow CaP filaments. In addition, the mechanical properties of porous CaP scaffolds could be tailored by adjusting their porosity, for example, compressive strengths of 4.8 ± 1.1 MPa at a porosity of ~82 vol %. The porous CaP scaffold showed good biocompatibility, which was assessed by in vitro cell tests, where several the cells adhered to and spread actively with the outer and inner surfaces of the hollow CaP filaments.

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Substrate‐Independent Coating with Persistent and Stable Antifouling and Antibacterial Activities to Reduce Bacterial Infection for Various Implants

Yuan

Qiu

Wang

et al. 2019

Adv Healthcare Materials

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Implantation of biomedical devices accompanying infections has caused severe problems to public health that require feasible solutions. In this study, a simple approach is reported to fabricate a antimicrobial and antifouling dual‐functional coating. This coating consists of a substrate‐independent layer‐by‐layer (LBL) film formed by poly (diallyldimethylammonium) (PDDA) and poly (styrenesulfonate) (PSS), where parts of PSS and PDDA are physically substituted by hetero‐bifunctional polyethylene glycol (PEG) ending with a carboxyl group and antimicrobial peptide (ε‐Poly‐l‐lysine, ε‐PL). This design (ε‐PL‐PEG‐(PDDA/PSS)9 coating) exhibits not only potent antimicrobial activity against Gram‐positive/negative bacteria but also superior antifouling activity on various substrates, including glass and plastic. Moreover, the antifouling and antibacterial performance can be maintained for a longer period of time under physiological environments even after physical damage of the surface due to the homogeneous interspersion and free migration of ε‐PL‐PEG‐COOH in the LBL film. This allows the supplement of these molecules to the surface against molecule loss during usage. Both in vitro and in vivo (rodent subcutaneous infection model) studies show obvious reduction of the bacteria on the coated substrate and in the surrounding tissues with up to 3.2‐log reduction, even after repeated usage. The inflammation around the implantation area is also significantly inhibited.

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Preparation and Antibacterial Properties of Silver-Doped Nanoscale Hydroxyapatite Pastes for Bone Repair and Augmentation

Cited by 27 publications

References 0 publications

Electrospun Scaffolds Containing Silver-Doped Hydroxyapatite with Antimicrobial Properties for Applications in Orthopedic and Dental Bone Surgery

Electrospun Scaffolds Containing Silver-Doped Hydroxyapatite with Antimicrobial Properties for Applications in Orthopedic and Dental Bone Surgery

Coextrusion-Based 3D Plotting of Ceramic Pastes for Porous Calcium Phosphate Scaffolds Comprised of Hollow Filaments

Substrate‐Independent Coating with Persistent and Stable Antifouling and Antibacterial Activities to Reduce Bacterial Infection for Various Implants

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