Preparation and antibacterial activity of lysozyme and layered double hydroxide nanocomposites

Yang, Qinzheng; Chang, Ying-Yue; Zhao, Huazhang

doi:10.1016/j.watres.2013.09.002

Cited by 50 publications

(27 citation statements)

References 35 publications

(1 reference statement)

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“…This is the reason why natural antibacterial agents (antimicrobial peptides, lysozyme, chitosan) loaded into LDH have also been investigated. Mg 2 Al intercalated with lysozyme (LYZ) (LDH‐LYZ 0.8 w : w) was very efficient to remove more than 97.7 % of staphylococcus Aureus from water, maintaining its sterilization efficiency up to the 7th batch cycle. The reasons for such a high biocide performance are explained by the chemical stability of LYZ anchored at the layered structure and the ability of LDH and hybrid LDH (ξ≫0) to adsorb at the bacteria surface (ξ<0).…”

Section: Ldh and Biological Cell Interactionssupporting

confidence: 76%

Interactions between Biological Cells and Layered Double Hydroxides: Towards Functional Materials

Forano

Bruna

Mousty

et al. 2018

The Chemical Record

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This review highlights the current research on the interactions between biological cells and Layered Double Hydroxides (LDH). The as-prepared biohybrid materials appear extremely attractive in diverse fields of application relating to health care, environment and energy production. We describe how thanks to the main features of biological cells and LDH layers, various strategies of assemblies can be carried out for constructing smart biofunctional materials. The interactions between the two components are described with a peculiar attention to the adsorption, biocompatibilization, LDH layer internalization, antifouling and antimicrobial properties. The most significant achievements including authors' results, involving biological cells and LDH assemblies in waste water treatment, bioremediation and bioenergy generation are specifically addressed.

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Section: Ldh and Biological Cell Interactionssupporting

confidence: 76%

Interactions between Biological Cells and Layered Double Hydroxides: Towards Functional Materials

Forano

Bruna

Mousty

et al. 2018

The Chemical Record

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“…Since LDHs are anionic clay‐like materials, LDH can adsorb negatively charged bacterial cell walls (Van Der Wal, Norde, Zehnder, & Lyklema, ). However, it depends on the zeta potential of LDH and varies with the species type that has been intercalated (Yang et al., ).…”

Section: Resultsmentioning

confidence: 99%

“…According to the study findings, no zone inhibition was seen in neat LDH intercalated with nitrate ions .Under natural pH conditions, the polypeptides of bacteria and viruses are composed of weakly acidic and basic groups (e.g., carboxyl and amino moieties), and the isoelectric point lies in between pH 3 and pH 4. Thus, there is a possibility to remove bacteria by adsorption to pure LDH with lower concentrations of bacterial suspensions such as 1 × 10 4(Yang, Chang, & Zhao, 2013).Since LDHs are anionic clay-like materials, LDH can adsorb negatively charged bacterial cell walls(Van Der Wal, Norde, Zehnder, & Lyklema, 1997). However, it depends on the zeta potential of LDH and varies with the species type that has been intercalated(Yang et al, 2013).…”

mentioning

confidence: 99%

“…Thus, there is a possibility to remove bacteria by adsorption to pure LDH with lower concentrations of bacterial suspensions such as 1 × 10 4(Yang, Chang, & Zhao, 2013).Since LDHs are anionic clay-like materials, LDH can adsorb negatively charged bacterial cell walls(Van Der Wal, Norde, Zehnder, & Lyklema, 1997). However, it depends on the zeta potential of LDH and varies with the species type that has been intercalated(Yang et al, 2013).…”

mentioning

confidence: 99%

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Effect of natural curcuminoids‐intercalated layered double hydroxide nanohybrid against Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis: A bactericidal, antibiofilm, and mechanistic study

et al. 2018

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The study aimed to determine the antibacterial/antibiofilm effect and mechanism of interaction of curcuminoids-intercalated Mg/Al layered double hydroxide (curcuminoids-LDH) against three different bacteria. Antimicrobial effect of curcuminoids-LDH nanohybrid was investigated against P. aeruginosa, S. aureus, and E. faecalis (for both standard strains and clinical isolates), using agar well diffusion method. Minimum inhibitory concentrations (MIC) of planktonic bacteria were determined using the broth microdilution method. MIC of biofilms (MBIC ) and killing time for 48 hr matured biofilms were determined by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Scanning electron microscopy (SEM) was used to determine pre- and postexposure architecture of biofilms. The mechanism of the antibiofilm activity of curcuminoids-LDH was determined using UV-visible spectroscopy. All tested bacteria had given a zone of inhibition in the presence of curcuminoids-LDH. The MIC values were 0.200 g/ml for P. aeruginosa, 0.025 g/ml for S. aureus, and 0.100 g/ml for E. faecalis. The 48 hr matured biofilms were reduced by curcuminoids-LDH with an MBIC of 0.100 g/ml. The minimum time to achieve MBIC was 3 hr, and the reduction was constant until 48 hr. SEM images showed a significant reduction of biofilm cell density and exopolymer matrics for all biofilms in the presence of curcuminoids-LDH. UV-visible studies revealed the antibiofilm activity of curcuminoids-LDH as due to the auto-oxidation of curcuminoids. The oxidation products are more limited in both product concentration per unit time and the variety of products, compared to pure curcuminoids, resulting in sharper UV-visible peaks than in the case of the latter. Curcuminoids-LDH has a potential antibacterial activity against P. aeruginosa, S. aureus, and E. faecalis. An antibiofilm activity has been achieved within 3 hr of the treatment. Curcuminoids released from the LDH showed the antibacterial activity due to oxidation products interfering with bacterial cell functions, and also encapsulation in the LDH causes curcuminoids to exhibit the activity in a persistent manner compared to pure curcuminoids.

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“…This was due to the double action of LYZ LDHs, ie, adsorption of LDHs and antibacterial action of LYZ. 73 The LYZ LDH formulation is considered to be a green antibacterial agent, as it does not produce any by-product.…”

Section: Application Of Layered Double Hydroxides Catalyticmentioning

confidence: 99%

Inorganic nanolayers: structure, preparation, and biomedical applications

Saifullah¹,

Hussein²

2015

IJN

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Hydrotalcite-like compounds are two-dimensional inorganic nanolayers also known as clay minerals or anionic clays or layered double hydroxides/layered hydroxy salts, and have emerged as a single type of material with numerous biomedical applications, such as drug delivery, gene delivery, cosmetics, and biosensing. Inorganic nanolayers are promising materials due to their fascinating properties, such as ease of preparation, ability to intercalate different type of anions (inorganic, organic, biomolecules, and even genes), high thermal stability, delivery of intercalated anions in a sustained manner, high biocompatibility, and easy biodegradation. Inorganic nanolayers have been the focus for researchers over the last decade, resulting in widening application horizons, especially in the field of biomedical science. These nanolayers have been widely applied in drug and gene delivery. They have also been applied in biosensing technology, and most recently in bioimaging science. The suitability of inorganic nanolayers for application in drug delivery, gene delivery, biosensing technology, and bioimaging science makes them ideal materials to be applied for theranostic purposes. In this paper, we review the structure, methods of preparation, and latest advances made by inorganic nanolayers in such biomedical applications as drug delivery, gene delivery, biosensing, and bioimaging.

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Preparation and antibacterial activity of lysozyme and layered double hydroxide nanocomposites

Cited by 50 publications

References 35 publications

Interactions between Biological Cells and Layered Double Hydroxides: Towards Functional Materials

Interactions between Biological Cells and Layered Double Hydroxides: Towards Functional Materials

Effect of natural curcuminoids‐intercalated layered double hydroxide nanohybrid against Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis: A bactericidal, antibiofilm, and mechanistic study

Inorganic nanolayers: structure, preparation, and biomedical applications

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Preparation and antibacterial activity of lysozyme and layered double hydroxide nanocomposites

Cited by 50 publications

References 35 publications

Interactions between Biological Cells and Layered Double Hydroxides: Towards Functional Materials

Interactions between Biological Cells and Layered Double Hydroxides: Towards Functional Materials

Effect of natural curcuminoids‐intercalated layered double hydroxide nanohybrid against Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis: A bactericidal, antibiofilm, and mechanistic study

Inorganic nanolayers: structure, preparation, and&nbsp;biomedical applications

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Inorganic nanolayers: structure, preparation, and biomedical applications