Preparation and analysis of macroporous TiO2 films on Ti surfaces for bone-tissue implants

Abstract

This article describes the preparation and analysis of macroporous TiO2 films on Ti surfaces, for application in bone tissue-Ti implant interfaces. These TiO2 bioceramic films have a macroporous structure consisting of monodisperse, three-dimensional, spherical, interconnected pores adjustable in the micron size range. Micron-sized polystyrene (PS) bead templates are used to precisely define the pore size, creating macroporous TiO2 films with 0.50, 16, and 50 microm diameter pores, as shown by scanning electron microscopy. X-ray photoelectron spectroscopy shows the films to be predominantly composed of TiO2, with approximately 10% carbon. X-ray diffraction reveal rutile as the main phase when fired to the optimal temperature of 950 degrees C. Preliminary experiments find that the in vitro proliferation of human bone-derived cells (HBDC) is similar on all three pore sizes. However, higher [3H]thymidine incorporation by the HBDC is observed when they are grown on 0.50- and 16-microm pores compared to the 50-microm pores, suggesting an enhanced cell proliferation for the smaller pores.

Template-free synthesis of hierarchical porous SnO2

“…Up to now, different novel morphologies of SnO 2 , such as nanoparticles, nanorods, nanotubes, nanodiskettes, nanoboxes, hollow spheres, and mesoporous structures [14][15][16][17][18][19][20][21], have been designed. As known, the hierarchical porous material has been studied extensively for its wide applications such as HPLC separation, bone-tissue implants, catalysis, electrode materials for fuel cell application, heterogeneous colorimetric sensors, biomaterials engineering and controlled drug delivery devices, photovoltaic cells and energy conversion applications [22][23][24][25][26][27][28][29]. To the best of our knowledge, the production of hierarchical porous SnO 2 has never been reported before.…”

The relation between electrochemical tests and in vitro evaluation of titanium alloy biocompatibility

“…In the case of titanium and its alloys, the body does not produce this barrier; in contrast, permits the growth of the bone on the implant surface, in direct contact. The bone grows in all pores of the implant surface and becomes firmly bonded with this, ''the osseointegration'' process [16][17][18].…”

Investigation of formation processes of porous biocompatible materials based on silicon doped and undoped ?-calcium-phosphate and hydroxyapatite

“…135 Slično, manje pore (0.4 i 13 µm) u TiO 2 filmu na površini titana uslovljavaju povedanu proliferaciju humanih delija tripsinizovanih iz kostiju u poređenju sa vedim porama (49µm). 73 U kompozitnim skafoldima na bazi apatita i kolagena sa porama veličine od 50 do 300 µm, povedani sadržaj apatita (0, 70 i 90 mas.%) utiče na smanjenje poroznosti (79, 73 i 49 mas.%, redom) ali se ne primeduju značajnije razlike u proliferaciji MC3T3-E1 osteoblasta. 136 Istraživanja koja su vršena sa mezenhimalnim matičnim delijama pacova na neizatkanim vlaknima polietilen tereftalata različitog prečnika (2-42 µm) i poroznosti (93-97 %) su pokazala da je na vlaknima manjeg prečnika primedeno slabije vezivanje delija i sferni oblik delija, zbog toga što je veličina delija porediva ili čak veda od prečnika vlakna.…”

“…68,72 Biomimetička precipitacija je primedena na površini Si-HA i Si-α-TCP. 73 Potpuna transformacija u HA sličan biološkom apatitu uočena kod nekih Si-α-TCP materijala koji su držani u SBF-u. 73 Utvrđeno je da površina Si-α-TCP filmova pokazuje povedani afinitet prema OPN proteinima na mestima na kojima je uočeno taloženje HA.…”

“…73 Potpuna transformacija u HA sličan biološkom apatitu uočena kod nekih Si-α-TCP materijala koji su držani u SBF-u. 73 Utvrđeno je da površina Si-α-TCP filmova pokazuje povedani afinitet prema OPN proteinima na mestima na kojima je uočeno taloženje HA. 74 Mikroskopijom atomskih sila Si-HA i HA utvrđeno je da korišdenjem vrha koji se završava COO -grupom Si-HA pokazuje prisustvo komponenata sa jačim van der Waalsovim silama, elektronegativnije površine i povedanu površinsku adheziju.…”