Preparation and Analysis of Diagnostic Antipneumococcus Serum

Welch, Henry; Borman, Earle K.; Mickle, Friend Lee

doi:10.2105/ajph.29.1.35

Cited by 6 publications

(3 citation statements)

References 3 publications

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“…Reciprocal, as well as unilateral, cross-reactions were often encountered in early efforts to obtain a reliable supply of strictly type-specific, diagnostic rabbit anti-Pn sera (46,58). In this part of the present paper, an attempt is made to find out why some cross-precipitations between serological types are unilateral and others are reciprocal.…”

Section: Reciprocal Cross-reactionsmentioning

confidence: 89%

“…S32 and S32A (67 U.S.) (structures unknown). S32 contains Gal, Glc, Rham, and uronic acid (1) and does not precipitate as strongly in anti-Pn23 (a long-known cross-reaction [46,58]) as does S32A ( Table 1). The latter, at least, may therefore be assumed to have nonreducing lateral end groups of L-Rham in its repeating units (P. Allen, B. Prescott, and M. Heidelberger, unpublished data); phosphorylcholine is also a constituent.…”

Section: S25mentioning

confidence: 99%

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Precipitating cross-reactions among pneumococcal types

Heidelberger¹

1983

Infect Immun

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Data accumulated over many years are brought together on cross-reactions of 46 among the more than 80 pneumococcal serological types, with the idea of correlating cross-reactions with the structures of the relevant type-specific capsular polysaccharides, insofar as these have been determined. The precipitin reaction was carried out with the polysaccharides and antibodies raised in horses, rabbits, and a mule. Quantitative values (micrograms of antibody nitrogen per milliliter of antiserum at 0 to PC) are given in many instances and discussed, together with arbitrary qualitative data, in terms of the known structures of the polysaccharides. Some precise relationships are uncovered, and an attempt is made to determine why some of the cross-reactions are reciprocal and why others are only unilateral. were placed in a cold bath at 0 to PC for 2 days to 2 weeks or longer, depending on the rapidity of precipitation. Qualitative data are given within parentheses 1234 on September 3, 2020 by guest http://iai.asm.org/ Downloaded from CROSS-REACTIONS OF PNEUMOCOCCAL TYPES 1235 when carried out in antisera which were negative or weakly positive in other tests. Quantitative figures within parentheses refer to single analyses instead of the usual mean of duplicates. The following abbreviations are used: S, specific capsular polysaccharide; Gal, galactose; Glc, glucose; Rham, rhamnose; Man, mannose; GalA, galacturonic acid; GlcA, glucuronic acid; GlcNAc, N-acetylglucosamine; dp, depyruvylated. Except as indicated, the antisera used had been raised in horses. All sugars are in the pyranose form unless indicated as furanoses by f, as in Galf.

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Section: Reciprocal Cross-reactionsmentioning

confidence: 89%

Section: S25mentioning

confidence: 99%

Precipitating cross-reactions among pneumococcal types

Heidelberger¹

1983

Infect Immun

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“…Although a reciprocal cross-reaction between pneumococcal types 23 and 32 has been known for a long time (10,18), the structural basis for this antigenic relationship has never been elucidated. Pneumococcal type 23 polysaccharide consists of D-glucose, D-galactose, and L-rhamnose (data from Jones and Perry, [5]) present in 1:1:2 molar proportions ( Table 2).…”

Section: Downloaded Frommentioning

confidence: 99%

Immunochemical studies on a Mycoplasma pneumoniae polysaccharide fraction: cross-reactions with type 23 and 32 antipneumococcal rabbit sera

Allen¹,

Prescott²

1978

Infect Immun

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Lipid-free polysaccharide fraction 2 extracted from Mycoplasma pneumoniae strain FH by Prescott et al. (J. Bacteriol. 91:2117-2115, 1966 was examined for its ability to cross-precipitate antibody from type-specific rabbit antipneumococcal sera types 1 to 34 inclusive. Cross-precipitation in type-specific pneumococcal anti-type 23 and anti-type 32 sera was examined in detail and could be attributed to a rhamnose-galactose-rich component of crude M. pneumoniae polysaccharide fraction 2 recovered from immunoprecipitates formed with anti-type 23 serum. Immunochemically isolated mycoplasma polysaccharide was found to contain glucose, galactose, rhamnose, and mannose in 1:14:5:4 molar proportions. Comparison of the ability of 6-0-a-L-rhamnosyl-D-glucose and free L-rhamnose to inhibit precipitation by homologous pneumococcal and heterologous mycoplasma polysaccharide antigens indicates a combining site specificity for anti-type 23 and anti-type 32 antibodies directed largely against the a-linked L-rhamnosyl determinants and the occurrence of a-L-rhamnosyl units in type 32 and M. pneumoniae polysaccharides. Hapten inhibition of the cross-precipitation of pneumococcal type 23 capsular polysaccharide in anti-type 32 serum helps to establish that cross-reactivity can be attributed to interaction of recurrent, a-L-rhamnosyl units of type 23 with anit-a-L-rhamnoside combining sites of anti-type 32 antibodies.

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The Present Status of the Higher Types of Antipneumococcus Serums

Finland¹

1942

JAMA

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Preparation and Analysis of Diagnostic Antipneumococcus Serum

Cited by 6 publications

References 3 publications

Precipitating cross-reactions among pneumococcal types

Precipitating cross-reactions among pneumococcal types

Immunochemical studies on a Mycoplasma pneumoniae polysaccharide fraction: cross-reactions with type 23 and 32 antipneumococcal rabbit sera

The Present Status of the Higher Types of Antipneumococcus Serums

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