Prenatal Exposure to Hypoxia Induced Beclin 1 Signaling-Mediated Renal Autophagy and Altered Renal Development in Rat Fetuses

Xia, Shuixiu; Lv, Jian; Gao, Qinqin; Chen, Ningjing; Wei, Xiaoguang; Xiao, Jia; Chen, Jie; Tao, Jianying; Sun, Miao; Mao, Caiping; Zhang, Li; Xu, Zhice

doi:10.1177/1933719114536474

Cited by 34 publications

(37 citation statements)

References 48 publications

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“…Our previous study demonstrated that high-salt (HS) diets during pregnancy could affect local renin-angiotensin system in the fetal and offspring kidney [7]. In addition, recent work demonstrated that the prenatal insult could affect renal development [8]. However, there has been very limited information about whether the function of renal interlobar arteries can be influenced by prenatal HS intake.…”

Section: Introductionmentioning

confidence: 97%

High-salt diets during pregnancy increases renal vascular reactivity due to altered soluble guanylyl cyclase-related pathways in rat offspring

Lin

Yin

et al. 2016

The Journal of Nutritional Biochemistry

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Section: Introductionmentioning

confidence: 97%

High-salt diets during pregnancy increases renal vascular reactivity due to altered soluble guanylyl cyclase-related pathways in rat offspring

Lin

Yin

et al. 2016

The Journal of Nutritional Biochemistry

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“…The observed increase in co-localization between LC3 and LAMP2 suggested that autophagic flux was increased and more autophagosomes fused with the lysosomes for degradation 92,93 . Hypoxia during in utero development also led to autophagy induction and apoptosis in the kidney with alteration in Beclin 1, hypoxia inducible factor 1alpha (HIF-1α), AKT, and mTOR signaling 95 . The mechanism by which ischemia up-regulates autophagy is under intense investigation.…”

Section: Introductionmentioning

confidence: 99%

Autophagy and Tubular Cell Death in the Kidney

Havasi

Dong

2016

Seminars in Nephrology

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Summary Many common renal insults such as ischemia and toxic injury primarily target the tubular epithelial cells especially the highly metabolically active proximal tubular segment. Tubular epithelial cells are particularly dependent on autophagy to maintain homeostasis and respond to stressors. The pattern of autophagy in the kidney has a unique spatial and chronological signature. Recent evidence shows that there is a complex crosstalk between autophagy and various cell death pathways. The purpose of this review is to specifically discuss the interplay between autophagy and cell death in the renal tubular epithelia. It is imperative to review this topic because recent discoveries have improved our mechanistic understanding of the autophagic process and have highlighted its broad clinical applications, making autophagy a major target for drug development.

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“…Despite the enhanced intestinal autophagy, IUGR pups still had higher mRNA expression of caspase 9 and lower level of Bcl-2 mRNA expression in present study, which indicated that intestinal apoptosis has been up-regulated [42,43] . The over-induced apoptosis might lead to further organ damage [44] . For example, Xia et al [44] proposed that hypoxia induced renal autophagy via Beclin1 signal pathway, enhanced apoptosis and affected the renal development in IUGR rat fetuses.…”

Section: Discussionmentioning

confidence: 99%

“…The over-induced apoptosis might lead to further organ damage [44] . For example, Xia et al [44] proposed that hypoxia induced renal autophagy via Beclin1 signal pathway, enhanced apoptosis and affected the renal development in IUGR rat fetuses. We also observed the similar phenomenon in small intestine of IUGR fetuses (data not published) and newborn IUGR piglets (without feeding colostrum) that over-enhanced autophagy and apoptosis might Effects of IUGR on expression of apoptosis and proliferation related genes in the jejunum.…”

Section: Discussionmentioning

confidence: 99%

Kolostruma Cevap Veren Rat Yavrularında İntrauterin Büyüme Geriliği İntestinal Otofaji ve Proliferayonu Artırır

Wang¹,

Zhang²,

Siyal³

et al. 2017

Kafkas Univ Vet Fak Derg

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This study aimed to investigate responsible mechanisms for rapid intestinal catch-up growth in intrauterine growth restriction (IUGR) pups via analysis of autophagy, apoptosis and proliferation in a rat model. Twenty primiparous dams were assigned into two groups as 1) dams with feed ad libitum (Adlib) and 2) dams with 50% feed restriction from gestational day 10 to 21 to achieve normal birth weight (NBW) and IUGR pups, respectively. Litter size and pup weight were recorded at parturition and 8 pups were kept in each litter to have sufficient colostrum for 24 h. Subsequently, 2 pups from each litter were decapitated. Results indicated that feed restriction dams had similar litter size with rats in Adlib group although produced IUGR pups. Histological analysis indicated that IUGR rats had decreased villus height and surface area in jejunum. There was an accumulation of autophagosomes in jejunal mucosa of IUGR pups, however, the mitochondria and microvilli were unaffected. mRNA expressions of WIPI1, MAP1LC3B, Atg13, ULK1 and Beclin1 were increased, and mTOR expression was decreased in jejunum of IUGR, which also had lower Bcl-2 mRNA expression, increased caspase 9 and relative increased ki67 mRNA expression. Results suggested that after feeding colostrum, IUGR pups had impaired jejunum with unaffected mitochondrial histology. Enhanced intestinal autophagy under low-stress conditions might improve intestinal proliferation, which may be contributed to the rapid intestinal catch-up growth. Keywords: Autophagy, Colostrum, Intrauterine growth restriction, Intestinal proliferation, Apoptosis Kolostruma Cevap Veren Rat Yavrularında İntrauterin Büyüme Geriliği İntestinal Otofaji ve Proliferayonu Artırır ÖzetBu çalışmanın amacı, rat modeli üzerinde otofaji, apoptozis ve proliferasyonu incelemek suretiyle intrauterin büyümesi kısıtlanan (IUGR) yavrularda hızlı intestinal büyümeden sorumlu mekanizmaları araştırmaktır. Yirmi adet bir doğum yapmış anneler iki gruba ayrıldı; 1) ad libitum beslenen (Adlib) anneler ve 2) Gestasyonun 10 ile 21. günü arasında yemi %50 kısıtlanan anneler. Böylece normal doğum ağırlığı (NBW) olan yavrular ve IUGR yavrular elde edildi. Doğumda yavru sayısı ve ağırlıkları kaydedildi ve her batında 8 yavru 24 saat süresince yeterli kolostrum alması için annesi ile birlikte tutuldu. Sonrasında, her batından 2 yavruya dekapitasyon uygulandı. Elde edilen sonuçlar yemi kısıtlanan annelerin yavru sayıları ile Adlib grubun yavru sayılarının benzer olduğunu ancak IUGR yavrular ürettiğini gösterdi. Histolojik incelemede IUGR ratların jejunumda azalmış villus yüksekliğine ve yüzey alanına sahip olduğu belirlendi. IUGR yavruların jejunum mukozasında otofagozomların oluştuğu ancak mitokondri ve mikrovillusların etkilenmediği gözlemlendi. IUGR yavruların jejunumlarında WIPI1, MAP1LC3B, Atg13, ULK1 ve Beclin1 mRNA ekspresyonlarının arttığı ve mTOR ekspresyonunun azaldığı belirlendi. Bu hayvanlarda daha düşük Bcl-2 mRNA ekspresyonu, artmış caspase 9 ve orantısal olarak artmış ki67 mRNA ekspresyonu tespit edil...

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Prenatal Exposure to Hypoxia Induced Beclin 1 Signaling-Mediated Renal Autophagy and Altered Renal Development in Rat Fetuses

Abstract: Hypoxia-affected fetal renal development was associated with renal apoptosis and Beclin 1 signaling-mediated autophagy.

Cited by 34 publications

References 48 publications

High-salt diets during pregnancy increases renal vascular reactivity due to altered soluble guanylyl cyclase-related pathways in rat offspring

High-salt diets during pregnancy increases renal vascular reactivity due to altered soluble guanylyl cyclase-related pathways in rat offspring

Autophagy and Tubular Cell Death in the Kidney

Kolostruma Cevap Veren Rat Yavrularında İntrauterin Büyüme Geriliği İntestinal Otofaji ve Proliferayonu Artırır

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