Prenatal exposure to hexavalent chromium disrupts testicular steroidogenic pathway in peripubertal F1 rats

Navin, Ajit Kumar; Aruldhas, M. M.; Navaneethabalakrishnan, Shobana; Mani, K.; Prakash, Seppan; Banu, Sakhila K.

doi:10.1016/j.reprotox.2021.01.014

Cited by 16 publications

(21 citation statements)

References 65 publications

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“…In rats with prenatal exposure to Cr(VI), the vacuolation of mitochondria in Leydig cells [70] was also observed. A recent study suggested that prenatal exposure to Cr(VI) also decreased the expression of StAR protein in Leydig cells, which consequently led to the limited availability of cholesterol that is converted in mitochondria to pregnenolone [71]. Exposure to Cr(VI) also downregulated the steroidogenic enzymes and their specific activities involved in cholesterol conversion to progesterone, pregnenolone, progesterone, androstenedione and testosterone, respectively.…”

Section: Hormones Metabolismmentioning

confidence: 99%

“…Additionally, adverse effects on the expression of steroidogenic receptors for gonadotropins, prolactin, androgens and estrogens were also observed. These changes led to a decrease in testosterone biosynthesis, an increased serum concentration of luteinizing hormone, follicle-stimulating hormone, 17β-estradiol as well as a decrease in testosterone and prolactin in F1 rats with prenatal exposure to Cr(VI) [71].…”

Section: Hormones Metabolismmentioning

confidence: 99%

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HBM4EU Chromates Study: Urinary Metabolomics Study of Workers Exposed to Hexavalent Chromium

et al. 2022

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Exposure to hexavalent chromium Cr(VI) may occur in several occupational activities, placing workers in many industries at risk for potential related health outcomes. Untargeted metabolomics was applied to investigate changes in metabolic pathways in response to Cr(VI) exposure. We obtained our data from a study population of 220 male workers with exposure to Cr(VI) and 102 male controls from Belgium, Finland, Poland, Portugal and the Netherlands within the HBM4EU Chromates Study. Urinary metabolite profiles were determined using liquid chromatography mass spectrometry, and differences between post-shift exposed workers and controls were analyzed using principal component analysis. Based on the first two principal components, we observed clustering by industrial chromate application, such as welding, chrome plating, and surface treatment, distinct from controls and not explained by smoking status or alcohol use. The changes in the abundancy of excreted metabolites observed in workers reflect fatty acid and monoamine neurotransmitter metabolism, oxidative modifications of amino acid residues, the excessive formation of abnormal amino acid metabolites and changes in steroid and thyrotropin-releasing hormones. The observed responses could also have resulted from work-related factors other than Cr(VI). Further targeted metabolomics studies are needed to better understand the observed modifications and further explore the suitability of urinary metabolites as early indicators of adverse effects associated with exposure to Cr(VI).

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Section: Hormones Metabolismmentioning

confidence: 99%

Section: Hormones Metabolismmentioning

confidence: 99%

HBM4EU Chromates Study: Urinary Metabolomics Study of Workers Exposed to Hexavalent Chromium

et al. 2022

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Section: Resultsmentioning

confidence: 99%

“…According to previous research studies, Cr(III) is less toxic, rather a trace nutrient and desirable in water. According to USE, the Cr(VI) contaminant level is 0.1 ppm (Navin et al, 2021) The removal of Cr from real tannery effluent was also measured by AAS. The concentration of Cr as Cr(VI) and Cr(III) was found to decrease with time.…”

Section: Photocatalytic Reduction Of Cr(vi) Tocr(iii) In Real Tannery...mentioning

confidence: 99%

Enhanced Solar Photocatalytic Activity of Thermally Stable I:ZnO/Glass Beads for Reduction of Cr(VI) in Tannery Effluent

et al. 2022

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Chromium (VI) in tannery effluent is one of the major environmental concerns for the environmentalists due to the hazardous nature of Cr(VI) ions. To reduce Cr(VI) to Cr(III) as an innocuous moiety, pure and I-doped ZnO was grafted over the etched surface of glass beads by successive ionic layer adsorption and reaction (SILAR). Powdered, pure, and I-doped ZnO scrapped from the surface of glass beads was characterized for crystallinity, morphology, and elemental composition by XRD, SEM, TEM, and EDX. The optical properties of both photocatalysts revealed that owing to optimized iodine doping of ZnO, reduction in the bandgap was observed from 3.3 to 2.9 eV. The crystalline nano-bricks of I:ZnO adhered to glass beads were investigated to have remarkable capability to harvest sunlight in comparison to intrinsic ZnO nanodiscs. The thermal stability of I:ZnO was also found to be much improved due to doping of ZnO. The photocatalytic activities of ZnO/GB and I:ZnO/GB were compared by extent of reduction of Cr(VI) under direct natural sunlight (600–650 KWh/m2). The disappearance of absorbance peaks associated with Cr(VI) after treatment with I:ZnO/GB confirmed higher photocatalytic activity of I:ZnO/GB. The reaction parameters of solar photocatalytic reduction, i.e., initial pH (5–9), initial concentration of Cr(VI) (10–50 ppm), and solar irradiation time (1–5 h) were optimized using response surface methodology. The solar photocatalytic reduction of Cr(VI) to Cr(III) present in real tannery effluent was examined to be 87 and 98%, respectively, by employing ZnO/GB and I:ZnO/GB as solar photocatalysts. The extent of reduction was also confirmed by complexation of Cr(VI) and Cr(III) present in treated and untreated tannery waste with 1, 5-diphenylcarbazide. The results of AAS and UV/vis spectroscopy for the decrease in concentration of Cr also supported the evidence of higher efficiency of I:ZnO/GB for reduction of Cr(VI) in tannery effluent. Reusability of the fabricated photocatalyst was assessed for eight cycles, and magnificent extent of reduction of Cr(VI) indicated its high efficiency. Conclusively, I:ZnO/GB is a potential and cost-effective candidate for Cr(VI) reduction in tannery effluent under natural sunlight.

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“…Our data show that the HFD-HF diet decreased the expression of StAR protein in ovarian cells. This might have resulted in the limited availability of cholesterol inside the mitochondria to be converted to P4 [ 65 , 66 ]. Moreover, an increase in transcriptionally active β-catenin interacts with the StAR gene promoter and boosts StAR mRNA level and P4 synthesis [ 15 ], although it requires gonadotropins contribution to maximally impact steroidogenesis.…”

Section: Discussionmentioning

confidence: 99%

High Fat-High Fructose Diet Elicits Hypogonadotropism Culminating in Autophagy-Mediated Defective Differentiation of Ovarian Follicles

Rejani

Navin²,

Mumthaz³

et al. 2022

Cells

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Pituitary gonadotropins directly govern ovarian functions, which are in turn regulated by the ovarian steroid hormones. The precise interplay of gonadotropins and steroid hormones is critical for follicle growth and differentiation. Furthermore, autophagy regulates ovarian follicle differentiation. However, how the high-fat-high fructose (HFD-HF) diet regulates gonadotropins and facilitates autophagy-mediated follicular differentiation in the ovary is obscure. We fed prepubertal rats (PND 25) an HFD-HF diet until PND 90. The results showed diminished adenohypophyseal GnRHR, PR, and aromatase expression, whereas AR, ERα, PRLR, and inhibin were augmented, resulting in gonadotropins decline. Interestingly, autophagy biomarkers, Beclin-1, ATG5, ATG12, LC3-II, and LAMP1 were reduced but SQSTM1/p62 was augmented in the ovaries of HFD-HF-fed rats, causing autolysosome to aggregation. The diet altered T, E2, P4, PRL, and their receptors status in the ovary, disturbed estrous cyclicity, and delayed vaginal opening. Ovarian histomorphology exhibited numerous cystic and atretic follicles, along with disturbed follicular maturation and ovulation. Moreover, the reduction of FSHR; steroidogenic proteins; receptor proteins AR, Erβ, PR; and signaling proteins Wnt2 and β-catenin was also noticed in the ovary, whereas PRLR, inhibin, and pGSK3β were augmented. In conclusion, exposure to a prepubertal HFD-HF diet leads to hypogonadotropism and the autophagy-mediated defective differentiation of ovarian follicles, abating fertility in adult rats.

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Prenatal exposure to hexavalent chromium disrupts testicular steroidogenic pathway in peripubertal F1 rats

Cited by 16 publications

References 65 publications

HBM4EU Chromates Study: Urinary Metabolomics Study of Workers Exposed to Hexavalent Chromium

HBM4EU Chromates Study: Urinary Metabolomics Study of Workers Exposed to Hexavalent Chromium

Enhanced Solar Photocatalytic Activity of Thermally Stable I:ZnO/Glass Beads for Reduction of Cr(VI) in Tannery Effluent

High Fat-High Fructose Diet Elicits Hypogonadotropism Culminating in Autophagy-Mediated Defective Differentiation of Ovarian Follicles

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