Prenatal ethanol exposure potentiates isolation-induced ethanol consumption in young adult rats

Fernández, Macarena; Largo, Jorge Carrizo; Plaza, Wladimir; Haeger, Paola; Pautassi, Ricardo Marcos

doi:10.1016/j.alcohol.2018.05.006

Cited by 13 publications

(11 citation statements)

References 45 publications

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“…Consistent with these ethanol‐stress interactions, we recently reported greater anxiety‐like behavior in the light‐dark box paradigm (Di Doi, Fernández, Hager, & Pautassia, ), and greater isolation‐induced alcohol intake and preference, in PAE versus control adolescent rats (Fernández, Carrizo‐Largo, Plaza, Haeger, & Pautassi, ). Moreover, several clinical and pre‐clinical reports have indicated that individuals exposed to prenatal alcohol are more likely to develop drug abuse during adulthood (Baer, Sampson, Barr, Connor, & Streissguth, ; Contreras et al, ; Fabio et al, ; Malanga & Kosofsky, ; Miranda‐Morales, Nizhnikov, & Spear, ; Pautassi, Nizhnikov, Spear, & Molina, ).…”

Section: Introductionsupporting

confidence: 67%

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Pre‐ and postnatal alcohol exposure delays, in female but not in male rats, the extinction of an auditory fear conditioned memory and increases alcohol consumption

Plaza

Gaschino

Gutiérrez

et al. 2019

Developmental Psychobiology

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Repeated exposure to alcohol increases retrieval of fear‐conditioned memories, which facilitates, among other factors, the emergence of post‐traumatic stress disorder (PTSD). Individuals with PTSD are more likely to develop alcohol and substance abuse related disorders. We assessed if prenatal and early postnatal alcohol exposure (PAE) increased the susceptibility to retain aversive memories and if this was associated with subsequent heightened alcohol consumption. Pregnant Sprague‐Dawley rats were exposed for 22 hr/day, throughout pregnancy and until postnatal Day 7 to a single bottle of sucralose ‐ sweetened 10% alcohol solution (PAE Group), or to a single bottle of tap water and sucralose (Control Group). Auditory fear conditioning (AFC) was performed in the adolescent offspring at postnatal Day 40. Freezing was measured during acquisition, retention and extinction phases, followed by 3 weeks of free choice alcohol intake. Female, but not male, PAE rats exhibited impaired extinction of the aversive memory, a finding associated with higher levels of 3‐4 Dihidroxyphenylacetic acid (DOPAC) in the nucleus accumbens and heightened alcohol intake, respect to controls. These findings suggest that PAE makes females more vulnerable to long‐term retention of aversive memories, which coexist with heightened alcohol intake. These findings are reminiscent of those of PTSD.

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Section: Introductionsupporting

confidence: 67%

“…We followed the procedures of Contreras et al () and Fernández, Carrizo, Plaza, Haeger, and Pautassi (). Sprague‐Dawley female rats were exposed to water or alcohol (10% v/v, sweetened with 64 mg/L sucralose) throughout pregnancy and during the first 7 days of nursing (P7).…”

Section: Methodsmentioning

confidence: 99%

Pre‐ and postnatal alcohol exposure delays, in female but not in male rats, the extinction of an auditory fear conditioned memory and increases alcohol consumption

Plaza

Gaschino

Gutiérrez

et al. 2019

Developmental Psychobiology

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“…It is worth noting that in Fernández et al 14 we employed a similar PAE protocol, yet did not observe reduced overall liquid intake in the EtOH offspring. There are, however, some differences between the studies.…”

Section: Discussionmentioning

confidence: 84%

“…We recently found, in Sprague-Dawley rats, that isolation housing during adolescence unmasked the facilitative effect of PAE on alcohol intake at adulthood. 14 Based on this, we expected isolated housing to interact with PAE to promote alcohol drinking at adolescence, an effect likely to be associated with enhanced anxiety responses.…”

Section: E2mentioning

confidence: 99%

Effects of Isolated or Enriched Housing at Adolescence Upon Ethanol Intake and Anxiety Responses, In Rats Exposed to Prenatal Ethanol

Doi¹,

Fernández²,

Soto³

et al. 2019

J Fetal Alc Spect Disorder

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Abstract Prenatal alcohol exposure (PAE) is highly prevalent worldwide, and can affect alcohol intake and anxiety responses in the offspring. It is still relatively unknown how PAE interacts with stress exposure at adolescence, and there is a scarcity of treatments to reduce the impact of PAE. The present study assessed alcohol (ethanol) intake and anxiety responses after PAE and tested the modulation of these responses by different housing conditions during early adolescence. Pregnant dams were exposed for 22 hours/day, from gestational day 0, throughout pregnancy, and until postnatal day 7 (PD 7), to a single bottle of 10% ethanol that was mixed in tap water and sucralose (EtOH Group), or to a single bottle of tap water and sucralose (CTRL Group). During PDs 21-42 the offspring was exposed to standard pair housing, isolated housing or enriched housing. Immediately after, they were pair-housed and tested for ethanol intake in 24-hour access, intermittent 2-bottle choice sessions conducted over 4 weeks, and for anxiety responses in the light-dark box test. The EtOH offspring exhibited heightened anxiety (i.e., greater avoidance of the white area of the light-dark box) at PD21, when compared to control (CTRL) rats. Isolated housing induced greater avoidance of the white area, when compared to standard or enriched housing, on PDs 42 and 70. There were no significant EtOH versus CTRL differences in relative (i.e., percent preference vs. water) ethanol intake, yet overall fluid intake and absolute (g/kg) ethanol intake were significantly lower in EtOH versus CTRL rats. Enriched housing during adolescence had a suppressing effect upon absolute ethanol intake during the first week of testing, an effect more noticeable in CTRL than in PAE rats. The present study indicates that PAE or isolated housing is associated with an anxiety-like phenotype. The protracted PAE protocol here employed caused a generalized reduction of intake behaviours. Environmental enrichment was associated with a subtle, yet significant, reduction in absolute ethanol intake.

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“…Prolonged isolation housing during early development has also been shown to induce augmented alcohol intake in rodents (Lopez et al, 2011;Kutcher et al, 2016). Additionally, in one study it was found that this heightened alcohol intake was facilitated in adolescent males exposed to alcohol during gestation and the first week of lactation (Fernández et al, 2019). In that case, males pre-and postnatally exposed to alcohol showed significantly higher alcohol intake and increased alcohol preference in comparison with non-exposed controls during the first 5-6 testing sessions, but only if they had been housed in isolation conditions from weaning.…”

Section: Indirect Mechanismsmentioning

confidence: 99%

Prenatal Alcohol Exposure as a Case of Involuntary Early Onset of Alcohol Use: Consequences and Proposed Mechanisms From Animal Studies

Gaztañaga

Angulo-Alcalde

Chotro

2020

Front. Behav. Neurosci.

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Prenatal ethanol exposure potentiates isolation-induced ethanol consumption in young adult rats

Cited by 13 publications

References 45 publications

Pre‐ and postnatal alcohol exposure delays, in female but not in male rats, the extinction of an auditory fear conditioned memory and increases alcohol consumption

Pre‐ and postnatal alcohol exposure delays, in female but not in male rats, the extinction of an auditory fear conditioned memory and increases alcohol consumption

Effects of Isolated or Enriched Housing at Adolescence Upon Ethanol Intake and Anxiety Responses, In Rats Exposed to Prenatal Ethanol

Prenatal Alcohol Exposure as a Case of Involuntary Early Onset of Alcohol Use: Consequences and Proposed Mechanisms From Animal Studies

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