Prenatal Ethanol (EtOH) Exposure Alters the Sensitivity of the Adult Dentate Gyrus to Acute EtOH Exposure

Helfer, Jennifer L.; White, Emily R.; Christie, Brian R.

doi:10.1111/acer.12227

Cited by 5 publications

(4 citation statements)

References 52 publications

(62 reference statements)

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“…Neuroimaging studies also revealed overall brain volume reductions in FASD patients (Guerri et al, 2009). In agreement with human findings, rodent models of PAE have shown reductions in brain and body weight and reduced litter size in alcohol-exposed offspring (Abbott et al, 2016;An and Zhang, 2015;Chappell et al, 2007;Comeau et al, 2014;Dasgupta et al, 2007;El Shawa et al, 2013;Helfer et al, 2014). Nevertheless, other studies reported no differences in litter size or pup weight in rodent models of alcohol exposure in utero, which is consistent with our results (Allan et al, 2003;Barbier et al, 2009;Brady et al, 2012;Choi et al, 2005;Cullen et al, 2014;Hausknecht et al, 2014;Kleiber et al, 2011;Savage et al, 2002).…”

Section: Animal Model and Behavioral Effects Induced By Prenatal And supporting

confidence: 91%

Maternal alcohol binge drinking induces persistent neuroinflammation associated with myelin damage and behavioural dysfunctions in offspring mice

et al. 2017

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Alcohol binge drinking is on the increase in the young adult population, and consumption during pregnancy can be deleterious for foetal development. Maternal alcohol consumption leads to a wide range of long-lasting morphological and behavioural deficiencies known as foetal alcohol spectrum disorders (FASD), associated with neurodevelopmental disabilities. We sought to test the effects of alcohol on neuroimmune system activation and its potential relation to alcohol-induced neurodevelopmental and persistent neurobehavioural effects in offspring after maternal alcohol binge drinking during the prenatal period or in combination with lactation. Pregnant C57BL/6 female mice underwent a procedure for alcohol binge drinking either during gestation or both the gestation and lactation periods. Adult male offspring were assessed for cognitive functions and motor coordination. Early alcohol exposure induced motor coordination impairments in the rotarod test. Object recognition memory was not affected by maternal alcohol binge drinking, but Y-maze performance was impaired in pre- and early postnatal alcohol-exposed mice. Behavioural effects were associated with an upregulation of pro-inflammatory signalling (Toll-like receptor 4, nuclear factor-kappa B p65, NOD-like receptor protein 3, caspase-1, and interleukin-1β), gliosis, neuronal cell death and a reduction in several structural myelin proteins (myelin-associated glycoprotein, myelin basic protein, myelin proteolipid protein and myelin regulatory factor) in both the prefrontal cortex and hippocampus of adult mice exposed to alcohol. Altogether, our results reveal that maternal binge-like alcohol consumption induces neuroinflammation and myelin damage in the brains of offspring and that such effects may underlie the persistent cognitive and behavioural impairments observed in FASD.

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Section: Animal Model and Behavioral Effects Induced By Prenatal And supporting

confidence: 91%

Maternal alcohol binge drinking induces persistent neuroinflammation associated with myelin damage and behavioural dysfunctions in offspring mice

et al. 2017

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“…To induce LTD, we administered a train of low-frequency stimuli (LFS; 1 Hz; 900 pulses; Christie et al 1996). For LTP, short trains of high-frequency stimuli (HFS; 50 pulses at 100 Hz) were delivered four times with a 30-s intertrain interval (Helfer et al 2014). Following the application of conditioning stimuli, we resumed single-pulse stimulation (0.067 Hz) for an additional 60 min.…”

Section: Animalsmentioning

confidence: 99%

“…For instance, gonadotropin-releasing hormone (GnRH) can regulate estradiol synthesis, spine density, and the expression of synaptic proteins through both genomic and nongenomic mechanisms (Fester et al 2012;Kotitschke et al 2009). The mechanisms involved in spine generation are important to understand, because a number of sex-specific differences in synaptic plasticity have been reported in a variety of neuropathological processes that range from fetal alcohol spec-trum disorders (FASD) to Fragile-X syndrome (Bostrom et al 2015;Helfer et al 2012Helfer et al , 2014Yau et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…In the current study we first present a detailed protocol that uses the carbocyanine dye DiIC 18 to rapidly provide highresolution staining of dendritic spines from postnatal day (PND) 21 male and female rats prepared using procedures standard for in vitro electrophysiology (Christie et al 1997;Helfer et al 2014) or immunohistochemistry (Eadie et al 2005;Kannangara et al 2014). We then evaluate the effects of hippocampal slice preparation for electrophysiological experiments on dendritic spine density in juvenile males and females using this refined DiI procedure.…”

Section: Introductionmentioning

confidence: 99%

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Acute slice preparation for electrophysiology increases spine numbers equivalently in the male and female juvenile hippocampus: a DiI labeling study

Triviño-Paredes

Nahirney

Pinar

et al. 2019

Journal of Neurophysiology

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Hippocampal slices are widely used for in vitro electrophysiological experiments to study underlying mechanisms for synaptic transmission and plasticity, and there is a growing appreciation for sex differences in synaptic plasticity. To date, several studies have shown that the process of making slices from male animals can induce synaptogenesis in cornu ammonis area 1 (CA1) pyramidal cells, but there is a paucity of data for females and other brain regions. In the current study we use microcrystals of the lipophilic carbocyanine dye DiI (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate) to stain individual neurons in the CA1 and dentate gyrus (DG) hippocampal subfields of postnatal day 21 male and female rats. We show that the preparation of sections for electrophysiology produces significant increases in spines in sections obtained from females, similar to that observed in males. We also show that the procedures used for in vitro electrophysiology also result in significant spine increases in the DG and CA1 subfields. These results demonstrate the utility of this refined DiI procedure for staining neuronal dendrites and spines. They also show, for the first time, that in vitro electrophysiology slice preparations enhance spine numbers on hippocampal cells equivalently in both juvenile females and males. NEW & NOTEWORTHY This study introduces a new DiI technique that elucidates differences in spine numbers in juvenile female and male hippocampus, and shows that slice preparations for hippocampal electrophysiology in vitro may mask these differences.

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Alcohol Dehydrogenases and Acetaldehyde Dehydrogenases are Beneficial for Decidual Stromal Cells to Resist the Damage from Alcohol

et al. 2016

Alcohol and Alcoholism

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Prenatal Ethanol (EtOH) Exposure Alters the Sensitivity of the Adult Dentate Gyrus to Acute EtOH Exposure

Cited by 5 publications

References 52 publications

Maternal alcohol binge drinking induces persistent neuroinflammation associated with myelin damage and behavioural dysfunctions in offspring mice

Maternal alcohol binge drinking induces persistent neuroinflammation associated with myelin damage and behavioural dysfunctions in offspring mice

Acute slice preparation for electrophysiology increases spine numbers equivalently in the male and female juvenile hippocampus: a DiI labeling study

Alcohol Dehydrogenases and Acetaldehyde Dehydrogenases are Beneficial for Decidual Stromal Cells to Resist the Damage from Alcohol

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