Prenatal diagnosis with fetal cells isolated from maternal blood by multiparameter flow cytometry

Price, James O.; Elias, Sherman; Wachtel, Stephen S.; Klinger, Katherine W.; Dockter, Michael E.; Tharapel, Avirachan T.; Shulman, L.P.; Phillips, Owen P.; Meyers, Carole; Shook, Donna; Simpson, Joe Leigh

doi:10.1016/0002-9378(91)90024-l

Cited by 220 publications

(104 citation statements)

References 12 publications

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“…In the samples from the pregnant women, FISH with GMGY1O and DXZ1 showed four male pregnancies and one female pregnancy (figure D,F). Fetal sex determined in this way was in exact agreement with the cytogenetic analysis of amniotic fluid samples (table 3). …”

Section: Resultssupporting

confidence: 74%

Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting.

Zheng

Carter

Price

et al. 1993

Journal of Medical Genetics

122

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Fetal nucleated cells in the maternal circulation constitute a potential source of cells for the non-invasive prenatal diagnosis of fetal genetic abnormalities. We have investigated the use of the Magnetic Activated Cell Sorter (MACS) for enriching fetal nucleated erythrocytes. Mouse monoclonal antibodies specific for CD45 and CD32 were used to deplete leucocytes from maternal blood using MACS sorting, thus enriching for fetal nucleated erythrocytes which do not express either of these antigens. However, significant maternal contamination was present even after MACS enrichment preventing the accurate analysis of fetal cells by interphase fluorescence in situ hybridisation (FISH). To overcome this problem, we used simultaneous immunophenotyping of cells with the mouse antifetal haemoglobin antibody, UCHy, combined with FISH analysis using chromosome X and Y specific DNA probes. This approach enables selective FISH analysis of fetal cells within an excess of maternal cells. Furthermore, we have confirmed the potential of the method for clinical practice by a pilot prospective study of fetal sex in women referred for amniocentesis between 13 and 17 weeks of gestation.

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Section: Resultssupporting

confidence: 74%

Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting.

Zheng

Carter

Price

et al. 1993

Journal of Medical Genetics

122

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“…Normal human whole blood consists of red blood cells (RBCs) (5-9 × 10 9 ml −1 ), white blood cells (WBCs) (5-10 × 10 6 ml −1 ) and platelets (2.5-4 × 10 8 ml −1 ). The absolute number of fetal cells has yet to be established but their frequency has been estimated to be between one to two fetal cells per ml of maternal blood [26,27], or 1 in 10 5 -10 7 maternal cells [28]. The number of fetal cells has also been shown to increase in abnormal pregnancies which is believed to be due to an impaired placenta, leading to an increase in maternal-fetal transfusion [29,30].…”

Section: Prenatal Diagnosis and Fetal Cells In Maternal Bloodmentioning

confidence: 99%

“…The most commonly used antigens are Cluster of Differentiation, CD71 (transferrin receptor) [45], CD36 (thrombospondin receptor), glycophorin A (GPA) [46,28]. Fetal leukocytes were the first fetal cells to be separated by FACS in 1979 by Herzenberg et al [47], based on differences in human leukocyte antigen (HLA) expression.…”

Section: Flow Cytometry and Fluorescent Activated Cell Sorting (Facs)mentioning

confidence: 99%

Current and emerging techniques of fetal cell separation from maternal blood

Kavanagh

Kersaudy-Kerhoas

Dhariwal

et al. 2010

Journal of Chromatography B

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“…These concerns are the same for all rare cell applications, which include detection of CTCs, endothelial cells, hematopoetic stem cells (HSCs), and fetal cells in blood samples. 32–34 …”

Section: B Microfluidics and Microfabrication In Flow Cytometrymentioning

confidence: 99%

The intersection of flow cytometry with microfluidics and microfabrication

2014

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A modern flow cytometer can analyze and sort particles on a one by one basis at rates of 50,000 particles per second. Flow cytometers can also measure as many as 17 channels of fluorescence, several angles of scattered light, and other non-optical parameters such as particle impedance. More specialized flow cytometers can provide even greater analysis power, such as single molecule detection, imaging, and full spectral collection, at reduced rates. These capabilities have made flow cytometers an invaluable tool for numerous applications including cellular immunophenotyping, CD4+ T-cell counting, multiplex microsphere analysis, high-throughput screening, and rare cell analysis and sorting. Many bio-analytical techniques have been influenced by the advent of microfluidics as a component in analytical tools and flow cytometry is no exception. Here we detail the functions and uses of a modern flow cytometer, review the recent and historical contributions of microfluidics and microfabricated devices to field of flow cytometry, examine current application areas, and suggest opportunities for the synergistic application of microfabrication approaches to modern flow cytometry.

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Prenatal diagnosis with fetal cells isolated from maternal blood by multiparameter flow cytometry

Cited by 220 publications

References 12 publications

Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting.

Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting.

Current and emerging techniques of fetal cell separation from maternal blood

The intersection of flow cytometry with microfluidics and microfabrication

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