Prenatal betaine exposure alleviates corticosterone-induced inhibition of CYP27A1 expression in the liver of juvenile chickens associated with its promoter DNA methylation

Hu, Yun; Sun, Qinwei; Zong, Yibo; Liu, Jie; Idriss, Abdulrahman A.; Omer, Nagmeldin A.

doi:10.1016/j.ygcen.2016.12.014

Cited by 19 publications

(20 citation statements)

References 43 publications

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“…Previous study reported that prenatal betaine increased hepatic cholesterol content in newly hatched chicks ( Hu et al., 2015 ). In ovo injection of betaine did not affect hepatic cholesterol content but prevented corticosterone-induced increase in hepatic cholesterol content ( Hu et al., 2017 ). In the present study, we found that plasma and hepatic total cholesterol concentrations were significantly decreased in offspring of betaine-treated chickens, which was consistent with previous publication that dietary betaine supplementation reduced hepatic cholesterol content via improvement of bile acid metabolism in blunt-snout bream ( Wang et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Notably, dietary betaine supplementation in hens triggers the hypomethylation of SREBP2 and HMGCR genes promoter in offspring chickens, contributing to elevated hypothalamic cholesterol content ( Idriss et al., 2018 ). We previously reported that in ovo injection of betaine alleviates corticosterone-induced hepatic cholesterol deposition through epigenetic genes regulation in juvenile chickens ( Hu et al., 2017 ). However, it remains unknown whether epigenetic modifications were enrolled in the regulation of hepatic cholesterol metabolic gene transcripts in the offspring chickens when the hens were fed with betaine.…”

Section: Introductionmentioning

confidence: 99%

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Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes

Feng

Ding

et al. 2020

Poultry Science

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Maternal betaine was reported to regulate offspring hepatic cholesterol metabolism in mammals. However, it is unclear whether and how feeding betaine to laying hens affects hepatic cholesterol metabolism in offspring chickens. Rugao yellow-feathered laying hens ( n = 120) were fed basal or 0.5% betaine-supplemented diet for 28 D before the eggs were collected for incubation. Maternal betaine significantly decreased the hepatic cholesterol content ( P < 0.05) in offspring chickens. Accordingly, the cholesterol biosynthetic enzymes, sterol regulator element-binding protein 2 ( SREBP2 ) and 3-hydroxy-3-methylglutaryl coenzyme A reductase, were decreased, while cholesterol-7alpha-hydroxylase ( CYP7A1 ), which converts cholesterol to bile acids, was increased at both mRNA and protein levels in betaine-treated offspring chickens. Hepatic mRNA and protein expression of low-density lipoprotein receptor was significantly ( P < 0.05) increased, while the mRNA abundance of cholesterol acyltransferase 1 ( ACAT1 ) that mediates cholesterol esterification was significantly ( P < 0.05) decreased in the betaine group. Meanwhile, hepatic protein contents of DNA methyltransferases 1 and betaine homocysteine methyltransferase were increased ( P < 0.05), which was associated with modifications of CpG methylation on affected cholesterol metabolic genes. Furthermore, the level of CpG methylation on gene promoters was increased ( P < 0.05) for sterol regulator element-binding protein 2 and abundance of cholesterol acyltransferase 1 yet decreased ( P < 0.05) for cholesterol-7alpha-hydroxylase. These results indicate that maternal betaine supplementation significantly decreases hepatic cholesterol deposition through epigenetic regulation of cholesterol metabolic genes in offspring juvenile chickens.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes

Feng

Ding

et al. 2020

Poultry Science

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“…Betaine inhibits hepatic fat accumulation, and increases mitochondrial content and activity, which suggests that it is involved in the regulating lipid metabolism ( Zhang et al, 2019 ). Furthermore, in ovo betaine injection alleviates CORT-induced hepatic cholesterol deposition ( Hu et al, 2017 ). The fat metabolism function of laying hens is closely related to egg production because in the process of egg formation, yolk production requires a large amount of lipid deposition ( Burley et al., 1993 ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies in poultry have found that betaine supplementation may positively affect lipid metabolism by increasing fatty acid catabolism, thereby playing a key role in reducing carcass fat deposition ( García-Lezana et al, 2018 ). Incremental levels of betaine decreased LDL, TG, and TCH in broiler serum and effectively reduced abdominal fat deposition in a dose-dependent manner ( Hu et al., 2017 ). This may be why high doses of DMG resulted in lower abdominal fat in laying hens during the late laying period.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary dimethylglycine supplementation on laying performance, egg quality, and tissue index of hens during late laying period

Yao

Wang

et al. 2022

Poultry Science

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In this study, the effects of 5 graded dietary levels (0.025, 0.05, 0.075, 0.1, and 0.125%) of dimethylglycine ( DMG ) were studied in laying hens during the late laying period (71–78 wk). Graded doses of DMG from 0.025 to 0.125% in the diet produced quadratic positive ( P < 0.05) responses in the laying rate, egg-feed ratio, yolk color, grade follicular weight, and the number of large white follicles and linear positive ( P < 0.05) responses in average egg weight, and the number of large white follicles. With 0.1% DMG, the laying rate and egg-feed ratio improved ( P < 0.05), and the abdominal fat percentage decreased. Considering the laying performance under the conditions used in this study, the best-fit model for the DMG requirements of laying hens was estimated to range from 0.049 to 0.065% DMG during the late laying period based on a regression analysis. The addition of DMG did not affect the total cholesterol ( TCH ) and triglyceride ( TG ) contents in the plasma of laying hens; however, it significantly reduced the abdominal fat rate. DMG may change the course of lipid deposition in laying hens during the late laying period. In conclusion, supplementation with DMG can improve the laying rate and follicles development of laying hens.

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“…But this effect is not always consistent, and the effects on lipid profile vary with dietary formulations, dose of betaine, health, and stress ( 33 ). In a study, CORT downregulated LXR and CYP27A1 that contributed to cholesterol accumulation in juvenile chickens, but opposite results were demonstrated when CORT-induced embryos were supplemented with betaine (2.5 mg/egg at ED0) earlier ( 33 ). During HS, CORT secretion is increased and negatively changes the hepatic lipid profile.…”

Section: In Ovo Delivered Bioactive Compounds and Their Effectsmentioning

confidence: 99%

In ovo Feeding as a Tool for Improving Performance and Gut Health of Poultry: A Review

2021

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Early growth and development of the gastrointestinal tract are of critical importance to enhance nutrients' utilization and optimize the growth of poultry. In the current production system, chicks do not have access to feed for about 48–72 h during transportation between hatchery and production farms. This lag time affects early nutrient intake, natural exposure to the microbiome, and the initiation of beneficial stimulation of the immune system of chicks. In ovo feeding can provide early nutrients and additives to embryos, stimulate gut microflora, and mitigate the adverse effects of starvation during pre-and post-hatch periods. Depending on the interests, the compounds are delivered to the embryo either around day 12 or 17 to 18 of incubation and via air sac or amnion. In ovo applications of bioactive compounds like vaccines, nutrients, antibiotics, prebiotics, probiotics, synbiotics, creatine, follistatin, L-carnitine, CpG oligodeoxynucleotide, growth hormone, polyclonal antimyostatin antibody, peptide YY, and insulin-like growth factor-1 have been studied. These compounds affect hatchability, body weight at hatch, physiological functions, immune responses, gut morphology, gut microbiome, production performance, and overall health of birds. However, the route, dose, method, and time of in ovo injection and host factors can cause variation, and thereby inconsistencies in results. Studies using this method have manifested the benefits of injection of different single bioactive compounds. But for excelling in poultry production, researchers should precisely know the proper route and time of injection, optimum dose, and effective combination of different compounds. This review paper will provide an insight into current practices and available findings related to in ovo feeding on performance and health parameters of poultry, along with challenges and future perspectives of this technique.

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Prenatal betaine exposure alleviates corticosterone-induced inhibition of CYP27A1 expression in the liver of juvenile chickens associated with its promoter DNA methylation

Cited by 19 publications

References 43 publications

Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes

Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes

Effects of dietary dimethylglycine supplementation on laying performance, egg quality, and tissue index of hens during late laying period

In ovo Feeding as a Tool for Improving Performance and Gut Health of Poultry: A Review

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