Prenatal and postnatal changes in the content and species of ferritin in rat liver

Linder, Maria C.; Moor, Joan R.; Scott, Laura E.; Munro, Hamish N.

doi:10.1042/bj1290455

Cited by 37 publications

(15 citation statements)

References 23 publications

(31 reference statements)

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“…Newborn rat liver is known as a storage organ for ferritin iron, with very high concentrations (Linder et al, 1972), and this is supported by our results. This may be due to the demand of erythrocytes accompanied with the rapid growth from birth to weaning.…”

Section: Discussionsupporting

confidence: 90%

The effects of age and sex on seven elements in Sprague-Dawley rat organs

1990

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SummaryThis study reports age-related changes in 7 element (iron, copper, zinc, manganese, mercury, cadmium and lead) concentrations in the liver, kidney and brain of male and female Sprague-Dawley rats from 1 to 364 days of age. Atomic absorption spectrometry was used for the measurements.Copper, mercury and cadmium in the male and female kidneys increased from weaning until 127 days of age, as did iron concentrations in the female liver and kidney. After 127 days, especially, the copper concentration in the female kidney and cadmium concentration in the male and female kidney increased further. Consistent and statistically significant ( P < 0 .05) sex differences in element concentrations were found for three elements (iron, copper and zinc). Except for the zinc concentration in the liver from SO to 72 days, iron (in liver and kidney), zinc (in kidney) and copper (in liver, kidney and brain) concentrations in female rats during the adult stage, were all higher than those of male rats. Isolated differences for other elements (manganese, mercury and cadmium) were also found.The data will be helpful when setting up longterm animal investigations of the biological effect of elements. Keywords: Rat; Ageing; Sex difference; Trace element; Atomic absorption spectrometryIn animal experiments, age and sex are basic ·Present address: Tokyo University of Agriculture,

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Section: Discussionsupporting

confidence: 90%

The effects of age and sex on seven elements in Sprague-Dawley rat organs

1990

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“…If this hypothesis is correct, then under the influence of iron a subunit of the 'L' type which predominates in the more basic liver isoferritins may be synthesized at the expense of the 'H' type subunit characteristic of heart ferritin. The work of Linder et al (1972) lends support to this hypothesis, since they demonstrated that liver ferritin subunits in weanling rats differed from those of adult rat liver, but treatment with iron induced the formation of the adult liver ferritin type. The evidence of Konijn et al (1973) would indicate that the 'L' type subunits are those synthesized on free ribosomes.…”

Section: Discussionmentioning

confidence: 81%

Alterations in tissue ferritins in iron storage disorders.

Powell¹,

McKeering²,

Halliday³

1975

Gut

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SUMMARY Purified tissue ferritins isolated from Bantu subjects with gross haemosiderosis, from a patient with idiopathic haemochromatosis ( HC) treated by phlebotomy, and from rats with experimental iron overload were studied in order to determine the significance of the abnormality previously demonstrated in tissue isoferritins in patients with IHC. The isoferritin profile of the tissues from the Bantu subjects and the iron-loaded rats showed a similar abnormality to that previously found in patients with untreated IHC-that is, an abnormally uniform distribution of iron-containing isoferritins with an increase in the more basic isoferritins and an apparent absence of the more acidic ones. In contrast, tissues from the patient with treated IHC, who was iron depleted at the time of death, showed the normal organ-specific isoferritin distribution. These findings strongly suggest that the abnormal distribution of tissue isoferritins in IHC is an acquired phenomenon and unlikely to be related to an underlying genetic defect in ferritin or iron metabolism.Ferritin is the major iron storage protein in mammalian tissues and is found, or may be induced by iron administration, in most organs (Granick, 1951;Munro and Drysdale, 1970). Many tissue ferritins have been resolved into multiple isoferritins by the technique of isoelectric focusing Urushizaki et al., 1971;Drysdale, 1974;Powell et al., 1975). These isoferritins do not represent differences in aggregation or iron content of the protein but intrinsically different apoferritin shells 1974). Each tissue appears to have a characteristic isoferritin composition (Drysdale, 1974;Powell et al., 1975). This organ-specific isoferritin profile has recently been shown to be markedly abnormal in patients with untreated primary idiopathic haemochromatosis . The present study was designed to determine whether the abnormality in tissue isoferritins in idiopathic haemochromatosis is directly related to the genetic defect in iron metabolism or the result of long-standing iron overload. Methods HUMAN TISSUESNecropsy tissues were obtained from a normal adult male accident victim and also from a patient with classical familial idiopathic haemochromatosis whose case history and family history have been documented previously (Powell, 1965). This patient Received for publication 18 September 1975. had been treated by repeated phlebotomy for 11 years and was in a state of marginal iron deficiency at the time of his death at the age of 74 years. Histological examination showed no stainable iron in any of the tissues examined. Tissues from two Bantu patients with gross haemosiderosis were kindly provided by Professor R. Charlton of Johannesburg. ANIMAL STUDIESTen male Sprague Dawley rats weighing 250 to 300 g were each given 1 g/kg body weight of iron as irondextran by intramuscular injection over a period of two weeks. The rats were maintained on a normal diet tor three months and then killed by exsanguination from the dorsal aorta under ether anaesthesia. The carcasses were then perfuse...

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“…For 12 of the dams, the stage of pregnancy was timed. In the case of nontimed dams, pup gestational age was determined by crown-to-rump length and weight, comparing values with those from the timed pups, as described previously (2). Rats were infused intravenously into the tail vein with 67Cu-labeled samples under light pentobarbital anesthesia ( I 5 mg/kg ip) and were sacrificed by exsanguination under heavy pentobarbital anesthesia (50 mg/kg), with heparin treatment (48).…”

Section: Methodsmentioning

confidence: 99%

Ceruloplasmin and Copper Transport During the Latter Part of Gestation in the Rat

Lee¹,

Lancey²,

Montaser³

et al. 1993

Experimental Biology and Medicine

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We have examined the tissue uptake of 67Cu from ceruloplasmin versus albumin and transcuprein, after its intravenous administration to pregnant rats, in the last 4 days of gestation. 67Cu infused as in vivo-labeled ceruloplasmin remained on ceruloplasmin in the maternal circulation over the 4- to 6-hr time period examined, as determined by gel chromatography and immunoreactivity. That infused as in vitro-labeled serum was initially on transcuprein and albumin but soon also with new ceruloplasmin. On the basis of percent dose as well as total actual Cu transferred (taking into account the sizes of the two plasma Cu pools), ceruloplasmin was the preferred source of Cu for most tissues. Total uptake of Cu from ceruloplasmin was seven times greater than that from albumin and transcuprein for the placenta, whole fetus, and fetal liver. It was 2- to 6-fold greater for other tissues (except liver and kidney). When synthesis of maternal 67Cu-ceruloplasmin (from 67Cu administered on albumin and transcuprein) was inhibited with cycloheximide, uptake by nonhepatic tissues was reduced markedly. In the fetal circulation, entering 67Cu was initially associated with transcuprein and alpha-fetoprotein (or albumin), but then also appeared with ceruloplasmin. Specific receptors for ceruloplasmin were detected on membranes from the placenta as well as fetal liver; mRNA for ceruloplasmin was detected on the endoplasmic reticulum-bound polyribosomes of placenta/yolk sac, and of fetal and maternal liver. We conclude that Cu destined for the fetus is delivered mainly or exclusively by ceruloplasmin. It may enter via placental receptors, arriving in fetal plasma in ionic form, for later incorporation into fetal ceruloplasmin. The importance of ceruloplasmin as a source of plasma Cu for nonhepatic organs is also confirmed.

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Prenatal and postnatal changes in the content and species of ferritin in rat liver

Cited by 37 publications

References 23 publications

The effects of age and sex on seven elements in Sprague-Dawley rat organs

The effects of age and sex on seven elements in Sprague-Dawley rat organs

Alterations in tissue ferritins in iron storage disorders.

Ceruloplasmin and Copper Transport During the Latter Part of Gestation in the Rat

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