Prenatal administration of multipotent adult progenitor cells modulates the systemic and cerebral immune response in an ovine model of chorioamnionitis

Klein, Luise; Ophelders, Daan; Hove, Daniël van den; Damoiseaux, Maurits; Rutten, Bart P. F.; Reutelingsperger, Chris; Schurgers, Leon J.; Wolfs, Tim G. A. M.

doi:10.1016/j.bbih.2022.100458

Cited by 2 publications

(4 citation statements)

References 85 publications

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“…Recent research indicates that during chorioamnionitis infection, prenatal administration of multipotent adult progenitor cells enhances ANXA1 expression in immune cells within the brain’s vascular system and barriers. This increase in ANXA1 helps protect the newborn brain from inflammation induced by infection and from additional pro-inflammatory damage in the neonatal period [ 27 ]. Neutrophils, which migrate to inflamed tissues in response to inflammatory signals, are the main source of anti-inflammatory ANXA1 .…”

Section: Discussionmentioning

confidence: 99%

Maternal Hypermethylated Genes Contribute to Intrauterine Growth Retardation of Piglets in Rongchang Pigs

Wu,

Wang,

et al. 2024

IJMS

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The placenta is a crucial determinant of fetal survival, growth, and development. Deficiency in placental development directly causes intrauterine growth retardation (IUGR). IUGR can lead to fetal growth restriction and an increase in the mortality rate. The genetic mechanisms underlying IUGR development, however, remain unclear. In the present study, we integrated whole-genome DNA methylation and transcriptomic analyses to determine distinct gene expression patterns in various placental tissues to identify pivotal genes that are implicated with IUGR development. By performing RNA-sequencing analysis, 1487 differentially expressed genes (DEGs), with 737 upregulated and 750 downregulated genes, were identified in IUGR pigs (H_IUGR) compared with that in normal birth weight pigs (N_IUGR) (p < 0.05); furthermore, 77 miRNAs, 1331 lncRNAs, and 61 circRNAs were differentially expressed. The protein–protein interaction network analysis revealed that among these DEGs, the genes GNGT1, ANXA1, and CDC20 related to cellular developmental processes and blood vessel development were the key genes associated with the development of IUGR. A total of 495,870 differentially methylated regions were identified between the N_IUGR and H_IUGR groups, which included 25,053 differentially methylated genes (DMEs); moreover, the overall methylation level was higher in the H_IUGR group than in the N_IUGR group. Combined analysis showed an inverse correlation between methylation levels and gene expression. A total of 1375 genes involved in developmental processes, tissue development, and immune system regulation exhibited methylation differences in gene expression levels in the promoter regions and gene ontology regions. Five genes, namely, ANXA1, ADM, NRP2, SHH, and SMAD1, with high methylation levels were identified as potential contributors to IUGR development. These findings provide valuable insights that DNA methylation plays a crucial role in the epigenetic regulation of gene expression and mammalian development and that DNA-hypermethylated genes contribute to IUGR development in Rongchang pigs.

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Section: Discussionmentioning

confidence: 99%

Maternal Hypermethylated Genes Contribute to Intrauterine Growth Retardation of Piglets in Rongchang Pigs

Wu,

Wang,

et al. 2024

IJMS

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“…Surgical instrumentation of fetuses (121 days of gestational age (dGA)), i.a. LPS injection (125 dGA), MAPC preparation and administration (127 dGA) and betamethasone (11.4 mg i.m., Celestone®; Schering Plough Labo NV, Heist-op-den-Berg, BE) administration 24 h before birth and additionally at 48 h before birth for the prenatal cohort were performed as previously reported [ 16 ]. At 132 dGA (term 147 dGA), which is comparable to 33 weeks of human gestation and lung development is in the saccular phase [ 15 ], fetuses were surgically delivered preterm, and either killed humanely immediately with pentobarbital (200 mg/kg i.v., AST Farma B.V., Oudewater, NL) or mechanically ventilated for 72 h prior to humane killing.…”

Section: Methodsmentioning

confidence: 99%

“…Twenty-two pregnant ewes were randomized to the postnatal study (n = 4 to 6/group): a smaller Sham-MAPC group of n = 4 was used in the postnatal study to exclude MAPC-induced alterations in the lungs in a control setting, since previous clinical and experimental studies extensively showed the safety of MAPC under control conditions [ 7 , 16 ]. Fetuses of the postnatal cohort received identical prenatal treatments to the fetuses of the prenatal cohort, according to their group allocation.…”

Section: Methodsmentioning

confidence: 99%

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Multipotent adult progenitor cells prevent functional impairment and improve development in inflammation driven detriment of preterm ovine lungs

Neuen,

Ophelders,

Widowski

et al. 2024

Regenerative Therapy

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Prenatal administration of multipotent adult progenitor cells modulates the systemic and cerebral immune response in an ovine model of chorioamnionitis

Cited by 2 publications

References 85 publications

Maternal Hypermethylated Genes Contribute to Intrauterine Growth Retardation of Piglets in Rongchang Pigs

Maternal Hypermethylated Genes Contribute to Intrauterine Growth Retardation of Piglets in Rongchang Pigs

Multipotent adult progenitor cells prevent functional impairment and improve development in inflammation driven detriment of preterm ovine lungs

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