Premature ovarian insufficiency and autoimmune profiles: a prospective case–control study

Beitl, K.; Ott, J.; Rosta, K.; Holzer, I.; Foessleitner, P.; Steininger, J.; Panay, N.

doi:10.1080/13697137.2023.2287631

Cited by 4 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the time of examination, hypofunction of the thyroid gland was compensated in all patients by receiving L-thyroxine. The results obtained are consistent with the data of a recent study, which also showed a significant increase in the level of antithyroid antibodies in patients with POI (in 37.9% of cases) compared with healthy women [ 21 ]. According to 30 studies, women of reproductive age with Hashimoto’s thyroiditis are characterized by a decrease in AMH level and antral follicle count [ 22 ].…”

Section: Discussionsupporting

confidence: 92%

Diagnostic Value of Autoantibodies against Steroidogenic Enzymes and Hormones in Infertile Women with Premature Ovarian Insufficiency

Adamyan,

Menzhinskaya,

Antonova

et al. 2024

IJMS

View full text Add to dashboard Cite

The objective of the study was to evaluate the profile and diagnostic significance of serum autoantibodies in infertile patients with premature ovarian insufficiency (POI). The pilot study included 26 patients of reproductive age with POI and diminished ovarian reserve who received complex treatment using new surgical technologies (Group 1) and 18 patients without POI (Group 2). The profile of serum autoantibodies, including anti-ovarian antibodies, antibodies against thyroid peroxidase (TPO), steroidogenic enzymes, and steroid and gonadotropic hormones, was studied using modified ELISAs and human recombinant steroidogenic enzymes (CYP11A1, CYP19A1, CYP21A2). Patients in Group 1 had higher levels of IgG autoantibodies against steroidogenic enzymes, estradiol, progesterone, and TPO than those in Group 2. Tests for IgG antibodies against CYP11A1, CYP19A1, and CYP21A2 exhibited high sensitivity (65.4–76.9%), specificity (83.3–89.9%), and AUC values (0.842–0.910) for POI, the highest in the first test. Three-antibodies panel screening showed higher diagnostic accuracy (84.1% versus 75–79.6%). The levels of these antibodies correlated with menstrual irregularities and a decrease in the antral follicle count. Thus, antibodies against CYP11A1, CYP19A1, and CYP21A2 have a high diagnostic value for POI. Three-antibody panel screening may improve the accuracy of POI diagnosis and be useful for identifying high-risk groups, early stages of the disease, and predicting POI progression.

show abstract

Section: Discussionsupporting

confidence: 92%

Diagnostic Value of Autoantibodies against Steroidogenic Enzymes and Hormones in Infertile Women with Premature Ovarian Insufficiency

Adamyan,

Menzhinskaya,

Antonova

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

Profile of serum autoantibodies in women with premature ovarian insufficiency receiving complex treatment using new surgical technologies

Adamyan,

Menzhinskaya,

Antonova

et al. 2024

Rus. J. Hum. Repro.

View full text Add to dashboard Cite

Integrative Bioinformatics Analysis for Identifying the Mitochondrial-Related Gene Signature Associated with Immune Infiltration in Premature Ovarian Insufficiency

Lu,

Li,

Zhou

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Premature ovarian insufficiency (POI) is a reproductive disorder characterized by the cessation of ovarian function before the age of 40. While mitochondrial dysfunction and immune disorders are believed to contribute to ovarian damage in POI, the interplay between these factors remains understudied in patients with this condition. Methods In this research, transcriptomic data related to POI were obtained from the NCBI GEO database. Hub biomarkers were identified through the construction of a protein‒protein interaction (PPI) network and further validated using RT‒qPCR. Moreover, their expression across various cell types was elucidated via single-cell RNA sequencing analysis. Comprehensive investigation into the mitochondrial and immune profiles of POI patients was carried out through correlation analysis. Furthermore, potential therapeutic agents were predicted utilizing the cMap database. Results A total of 119 mitochondria-related differentially expressed genes (MitoDEGs) were pinpointed, showing significant enrichment in metabolic pathways. Among these genes, Hadhb, Cpt1a, Mrpl12, and Mrps7 were confirmed both in a POI model and in human granulosa cells (GCs), where they were found to accumulate in GCs and theca cells. Immune analysis revealed variations in macrophages, monocytes, and 15 other immune cell types between the POI and control groups. Notably, strong correlations were observed between seven hub-MitoDEGs (Hadhb, Cpt1a, Cpt2, Mrpl12, Mrps7, Mrps51, and Eci1) and various aspects such as mitochondrial respiratory complexes, dynamics, mitophagy, mitochondrial metabolism, immune-related genes, and immunocytes. Additionally, nine potential drugs (calyculin, amodiaquine, eudesmic acid, cefotaxime, BX-912, prostratin, SCH-79797, HU-211, and pizotifen) targeting key genes were identified. Conclusions Our results highlight the crosstalk between mitochondrial function and the immune response in the development of POI. The identification of MitoDEGs could lead to reliable biomarkers for the early diagnosis, monitoring and personalized treatment of POI patients.

show abstract

Premature ovarian insufficiency and autoimmune profiles: a prospective case–control study

Cited by 4 publications

References 29 publications

Diagnostic Value of Autoantibodies against Steroidogenic Enzymes and Hormones in Infertile Women with Premature Ovarian Insufficiency

Diagnostic Value of Autoantibodies against Steroidogenic Enzymes and Hormones in Infertile Women with Premature Ovarian Insufficiency

Profile of serum autoantibodies in women with premature ovarian insufficiency receiving complex treatment using new surgical technologies

Integrative Bioinformatics Analysis for Identifying the Mitochondrial-Related Gene Signature Associated with Immune Infiltration in Premature Ovarian Insufficiency

Contact Info

Product

Resources

About