Premature Chromosome Condensation (PCC) Assay for Dose Assessment in Mass Casualty Accidents

Lindholm, Carita; Stricklin, Daniela; Jaworska, A; Koivistoinen, A; Paile, W; Arvidsson, E.; Deperas‐Standylo, Joanna; Wójcik, Andrzej

doi:10.1667/rr1843.1

Cited by 66 publications

(44 citation statements)

References 22 publications

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“…The frequency of chromosome rings is in agreement with previously published data for 8 hours after exposure (30) but higher than that obtained for chemically induced PCC rings (31).…”

Section: Discussionsupporting

confidence: 92%

Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining

M’Kacher

Maalouf

Terzoudi

et al. 2015

International Journal of Radiation Oncology*Biology*Physics

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The introduction of TC staining to the PCC fusion technique has made possible the rapid scoring of unstable CAs, including dicentrics, with a level of accuracy and ease not previously possible. This new approach can be used for biological dosimetry in radiation emergency medicine, where the rapid and accurate detection of dicentrics is a high priority using automated scoring. Because there is no culture time, this new approach can also be used for the follow-up of patients treated by genotoxic therapy, creating the possibility to perform the estimation of induced chromosomal aberrations immediately after the blood draw.

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“…The frequency of chromosome rings is in agreement with previously published data for 8 hours after exposure (30) but higher than that obtained for chemically induced PCC rings (31).…”

Section: Discussionsupporting

confidence: 92%

Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining

M’Kacher

Maalouf

Terzoudi

et al. 2015

International Journal of Radiation Oncology*Biology*Physics

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“…Recently, the application of PCC assay has been expanded to not only exposure dose estimation of TBI but also dose estimation of PBI (19,35) and assessment of individual radiosensitivity in cancer patients (36). This optimized calyculin-A treatment provides an improved method for assessment of structural chromosome aberrations in interphase cells and can have broad implication for use in studies on mechanism of chromosome damage, screening of medical countermeasures for radiological protection, and radiation biodosimetry.…”

Section: Discussionmentioning

confidence: 99%

Optimization of calyculin A-induced premature chromosome condensation assay for chromosome aberration studies

Miura

Blakely

2011

Cytometry

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Calyculin A-induced premature chromosome condensation (PCC) assay is a simple and useful method to assess structural and numerical chromosome aberrations in cells. Our hypothesis in this study is that suboptimum calyculin A induction of PCC resulting in fuzzy compactness and/or shortened length chromosomes would decrease the detection sensitivity of numerical and structural chromosome aberrations such as small PCC rings and small excess fragments. In this study, an optimization of calyculin A exposure on chromosome morphology and PCC induction frequency was investigated using a human peripheral blood lymphocyte (PBL) ex vivo irradiation ( 60 Coc rays; $0.6 Gy/min; 0-30 Gy) model. Treatment with calyculin A (50 nM) for 15 and 30 min resulted in 11.3 AE 2.7 and 9.9 AE 1.6-fold increases in the frequency of G 2 /M-PCC cells with extended length chromosomes compared with the 60-min treated group over a broad dose range (0 to 20 Gy), respectively. The G 2 /M-PCC scoring index per PCC in 15-and 30-min treated groups was increased by 1.9 AE 0.2 (P 5 0.001) and 1.8 AE 0.2 (P 5 0.001) compared with the 60-min treated group over 0-20 Gy, respectively. The G 2 /M-PCC efficiency of 30-min treated group was highest in the three conditions (i.e., 15-, 30-, and 60-min treatment) of calyculin A exposure. Calyculin A (50 nM) treatment for 30 min before the 48-h harvest of mitogen-stimulated human PBL is optimum for the formation of suitable chromosome morphology necessary to assess structural chromosome aberrations induced by exposure to radiation using the chemical induced-PCC assay. Published 2011 Wiley Periodicals, Inc.

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“…This allows large doses (.5 Gy) to be estimated in comparison with the dicentric and micronucleus assays that work best at doses below 5 Gy owing to the saturation effect from high doses. PCC can score chromosomal rings in cases of high exposure, such as victims of the Tokaimura accident exposed to lethal doses [19], where it has been shown to be more effective than dicentric scoring [20]. Thanks to the avoidance of cell cycle delays and higher yields of scorable aberrations, this technique also has advantages over the dicentric assay in partial body exposure dosimetry [21] and in elderly or immune-compromised patients, and it can produce results quicker than the dicentric assay [17].…”

Section: Premature Chromosome Condensationmentioning

confidence: 99%

Deoxyribonucleic acid damage-associated biomarkers of ionising radiation: current status and future relevance for radiology and radiotherapy

Manning

Rothkamm²

2013

BJR

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Diagnostic and therapeutic radiation technology has developed dramatically in recent years, and its use has increased significantly, bringing clinical benefit. The use of diagnostic radiology has become widespread in modern society, particularly in paediatrics where the clinical benefit needs to be balanced with the risk of leukaemia and brain cancer increasing after exposure to low doses of radiation. With improving long-term survival rates of radiotherapy patients and the ever-increasing use of diagnostic and interventional radiology procedures, concern has risen over the long-term risks and side effects from such treatments. Biomarker development in radiology and radiotherapy has progressed significantly in recent years to investigate the effects of such use and optimise treatment. Recent biomarker development has focused on improving the limitations of established techniques by the use of automation, increasing sensitivity and developing novel biomarkers capable of quicker results. The effect of low-dose exposure (0-100 mGy) used in radiology, which is increasingly linked to cancer incidences, is being investigated, as some recent research challenges the linear-no-threshold model. Radiotherapy biomarkers are focused on identifying radiosensitive patients, determining the treatment-associated risk and allowing for a tailored and more successful treatment of cancer patients. For biomarkers in any of these areas to be successfully developed, stringent criteria must be applied in techniques and analysis of data to reduce variation among reports and allow data sets to be accurately compared. Newly developed biomarkers can then be used in combination with the established techniques to better understand and quantify the individual biological response to exposures associated with radiology tests and to personalise treatment plans for patients.

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Premature Chromosome Condensation (PCC) Assay for Dose Assessment in Mass Casualty Accidents

Cited by 66 publications

References 22 publications

Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining

Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining

Optimization of calyculin A-induced premature chromosome condensation assay for chromosome aberration studies

Deoxyribonucleic acid damage-associated biomarkers of ionising radiation: current status and future relevance for radiology and radiotherapy

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