Preliminary X-ray crystallographic data for the semisynthetic non-covalent complex of residues 1 to 118 and 111 to 124 of bovine pancreatic ribonuclease

Structural investigation of catalytically modified F120L and F120Y semisynthetic ribonucleases

“…The tetradecapeptide analogs were synthesized by the Wayne State University Macromolecular Core Facility and purified by methods previously described (Sasaki et al, 1979;Doscher et al, 1983b). Stock solutions of the peptides were characterized and standardized by amino acid analysis of acid hydrolysates of aliquot samples.…”

“…Crystals of RNase 1-118:lll-124 and the two analogs were all grown from ammonium sulfate/cesium chloride salt solutions buffered at pH 5.2 as described earlier (Sasaki et al, 1979;Doscher et al, 1983a). CsCl was removed by transferring the crystals into a stabilizing buffer of 80% saturated ammonium sulfate, 0.1 M ammonium acetate, pH 5.2.…”

Ribonuclease A

“…These studies were successful in illuminating molecular aspects of enzymatic catalysis, protein−protein interactions, and protein−nucleic acid interactions, and were the harbinger of current work on proteins containing variant or nonnatural amino acid residues. Work on the structure and function of another semisynthetic ribonuclease, RNase-(1−118)·(111−124), , has also made significant contributions. ,,,,− Recently, the RNase S system has spawned or at least facilitated the development of many innovative technologies.…”