Preliminary toxicity study of dichloromethane extract of Kielmeyera coriacea stems in mice and rats

Obici, Simoni; Otobone, Fernanda Jacques; Sela, Vânia Ramos; Ishida, Kelly; Silva, José Carlos da; Nakamura, Celso Vataru; Cortez, Diógenes Aparı́cio Garcia; Audi, Elisabeth Aparecida

doi:10.1016/j.jep.2007.09.013

Cited by 43 publications

(35 citation statements)

References 21 publications

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“…All observations were systematically recorded, with individual records being maintained for each animal. Cage side observation included evaluation of fur and skin, eyes, respiratory effects, autonomic effects such as salivation, diarrhea, urination and the central nerve effects including tremors and convulsions, changes in the level of activity, gait and posture, reactivity to handling or sensory stimuli and altered strength (Kumarnsit et al, 2006;Demma et al, 2006;Mukinda and Syce, 2007;Obici et al, 2008). Individual body weight was measured and recorded prior to the oral administration of extracts for 2 weeks.…”

Section: Cage Side Observation and Body Weight Measurementmentioning

confidence: 99%

“…Amount of food consumed was measured daily from the quantity of food supplied and the amount remaining after 24 h for 2 weeks of the study period (Kumarnsit et al, 2006;Demma et al, 2006;Obici et al, 2008 (Demma et al, 2006;Obici et al, 2008;Tan et al, 2008).…”

Section: Food Consumptionmentioning

confidence: 99%

“…Serum biochemistry was performed to examine Aspartate aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Creatinine (CRN), Triglycerides (TG), Cholesterol (Cho), Total Bilirubin (T-Bil), Blood Urea Nitrogen (BUN) and Blood Sugar (BS) (Demma et al, 2006;Obici et al, 2008;Tan et al, 2008).…”

Section: Biochemical Analysismentioning

confidence: 99%

“…The abdominal cavity of each animal was dissected and organs namely the heart, liver, lungs, spleen, kidneys, adrenals, thymus, salivary gland and brain were quickly removed, cleaned with saline, weighed and preserved in 10% formalin for histopathology analyses (Demma et al, 2006;Obici et al, 2008;Tan et al, 2008).…”

Section: Absolute and Relative Organ Weightmentioning

confidence: 99%

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Acute Toxicity Study of Tomato Pomace Extract in Rodent

Muchimapura

Tong-Un

Sripanidkulchai

2013

OnLine Journal of Biological Sciences

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Tomato and tomato products are considered to be healthy food for the human diet. Although tomatoes have been widely studied for their phenolic content, less emphasize has been laid on toxicological effect of this plant. Thus, the purpose of the present study is to determine the acute toxicity effect of Lycopersicon esculentum, or commonly known as tomato, was administered orally in the form of dried tomato pomace extract in vivo. Adult male rats were orally administrated single dose of 1000 and 5000 mg kg −1 dried tomato pomace extract. There were 10 rats in each group. All animals were sacrificed after 2 weeks of treatment. Seven parameters were tested: cage side observation, body weight gain measurement, food and water consumption, absolute organ weight, hematology, biochemical analysis and histopathology, to look for evidence of acute toxicity. No mortality was observed when varying doses of the extracts were administered per day for a period of 2 weeks. There were no significant differences in body weight, behavior, food consumption, absolute organ weights between controls and treated animals. Hematological analysis showed no differences in most parameters examined. In the biochemistry parameter measurement, no significant change occurred. Pathologically, neither gross abnormalities nor histopathological changes were observed. These finding suggest that none of the organs appeared to be target and the data could provide satisfactory preclinical evidence of safety to launch clinical trial on standardized formulation of tomato pomace extracts to be the dietary supplement.

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Section: Cage Side Observation and Body Weight Measurementmentioning

confidence: 99%

Section: Food Consumptionmentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

Section: Absolute and Relative Organ Weightmentioning

confidence: 99%

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Acute Toxicity Study of Tomato Pomace Extract in Rodent

Muchimapura

Tong-Un

Sripanidkulchai

2013

OnLine Journal of Biological Sciences

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“…It is worth noting that the intestinal absorption of plant extracts through the intestinal absorption is often low and weak (Obici et al, 2008).…”

Section: Fig 1: Animal Weights (G) With Time Of Treatmentmentioning

confidence: 99%

In vivo immunostimulatory effect of aqueous acetone extracts of Cienfuegosia digitata Cav. and Sida alba L. (Malvaceae) traditionally used to treat hepatitis B in Burkina Faso

Konaté¹,

Hilou²

2011

ABJNA

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In vivo immunostimulatory effect of aqueous acetone extracts of ABSTRACTThe present study was conducted to evaluate the immunostimulatory effect of aqueous acetone extracts of Cienfuegosia digitata Cav. and Sida alba L. (Malvaceae) on immunosuppressed wistar rats. These herbal plants are traditionally used in Burkina Faso to treat liver diseases. Animals received by gavage 50; 100 and 200 mg/kg bw of extracts daily for 14 days. Control groups received DMSO 10% and cyclosporin A. In vivo administration of cyclosporin A for 07 days resulted in an activity on hematological parameters (TWBC and total lymphocytes), serological parameters (CD4 and CD8) and animals body weights when compared with rats which received water (50, 100, 200 mg/kg bw; p<0.01). However, for test groups, it was noticed a decrease of the hematological and, serological parameters and also animals body weights comparatively to the control water group (p<0.01). We also noticed that extract at a dose of 200 mg/kg bw was highly effective than 50 and 100 mg/kg bw compared to the water control group (p<0.01). This study revealed that Cienfuegosia digitata has an immunostimulatory potential compared to Sida alba and these findings could justify the traditional use of the plant in the treatment of hepatitis B.

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Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

et al. 2017

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Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla extract. The phytochemical profile highlight the presence of flavonoids isorhamentin and quercetin derivates. The MTT assay was used to evaluate the cytotoxicity of different concentrations for different treatment times. Three concentrations (7.5, 15, 30 µg/mL) were chosen for assessment of apoptosis (AO/EB), mutagenicity (micronucleus), and cell cycle kinetics (flow cytometry). Thereafter, the concentration of 7.5 µg/mL was chosen to evaluate the protective effects against DNA damage induced by benzo[a]pyrene (B[a]P). At concentrations higher than 7.5 µg/mL (between 10 and 50 µg/mL), the extract was cytotoxic, induced apoptosis, and caused antiproliferative effects. However, it did not induce micronucleus and a reduction of apoptotic and micronucleated cells was observed in treatments that included the extract and B[a]P. The protective effect is attributable to the presence of flavonoids, described as antioxidants, inhibitors of DNA adduct and activators of detoxifying enzymes. The results of the present study such as absence of cytotoxic and mutagenic effects and protective effects against known carcinogens suggest that B. holophylla has potential for use soon as herbal medicine.

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Preliminary toxicity study of dichloromethane extract of Kielmeyera coriacea stems in mice and rats

Cited by 43 publications

References 21 publications

Acute Toxicity Study of Tomato Pomace Extract in Rodent

Acute Toxicity Study of Tomato Pomace Extract in Rodent

In vivo immunostimulatory effect of aqueous acetone extracts of Cienfuegosia digitata Cav. and Sida alba L. (Malvaceae) traditionally used to treat hepatitis B in Burkina Faso

Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

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