Preliminary survey of nematodes associated with broadleaved trees in İzmit Forest Management Directorate, Turkey

Abstract

A survey for Bursaphelenchus species was carried out in broadleaf forest sites of İzmit Forest Management Directorate. Nematodes were extracted from 128 wood samples, and 22 of them were cultured. Nematodes were identified using morphological characteristics and molecular analysis by amplifying D2‐D3 LSU with primers. Only one Bursaphelenchus species, Bursaphelenchus mucronatus kolymensis, was isolated from Quercus petraea representing the first report from broadleaved tree species in Turkey. Paraphelenchus acontioides and Aphelenchoides heidelbergi were identified and presented herein as the first reports of these two species from Turkey. In addition, five Aphelenchoides species were also found and reported as Aphelenchoides sp.