Preliminary survey of airborne fungal propagules in Turin, Italy

Marchisio, V. Filipello; Nosenzo, Carolina; Caramiello, Rosanna

doi:10.1016/s0953-7562(09)80977-5

Cited by 17 publications

(1 citation statement)

References 26 publications

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“…So, the proximity and interaction with air propagules especially in enclosed environments are much more than the soil propagules (Klich 2009). Aspergillus spores are important not only for initiating allergic reactions (rhinitis, asthma, and sinusitis) and superficial to deadly deep mycoses in various hosts such as mammals, birds, and even sea fans but also for their potential to harbor lifethreatening mycotoxins (Marchisio et al 1992). Among Aspergilli, Aspergillus flavus is the dominant pathogen in patients with sinusitis and fungal keratitis, the second most common pathogenic species in nosocomial aspergillosis, and a potent plant pathogen and mycotoxin producer.…”

Section: Introductionmentioning

confidence: 99%

A survey on distribution and toxigenicity of Aspergillus flavus from indoor and outdoor hospital environments

et al. 2011

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In the present study, genetic diversity and mycotoxin profiles of Aspergillus flavus isolated from air (indoors and outdoors), levels (surfaces), and soils of five hospitals in Southwest Iran were examined. From a total of 146 Aspergillus colonies, 63 isolates were finally identified as A. flavus by a combination of colony morphology, microscopic criteria, and mycotoxin profiles. No Aspergillus parasiticus was isolated from examined samples. Chromatographic analyses of A. flavus isolates cultured on yeast extract-sucrose broth by tip culture method showed that approximately 10% and 45% of the isolates were able to produce aflatoxin B(1) (AFB(1)) and cyclopiazonic acid (CPA), respectively. Around 40% of the isolates produced sclerotia on Czapek-Dox agar. The isolates were classified into four chemotypes based on the ability to produce AF and CPA that majority of them (55.5%) belonged to chemotype IV comprising non-mycotoxigenic isolates. Random amplified polymorphic DNA (RAPD) profiles generated by a combination of four selected primers were used to assess genetic relatedness of 16 selected toxigenic and non-toxigenic isolates. The resulting dendrogram demonstrated the formation of two separate clusters for the A. flavus comprised both mycotoxigenic and non-toxigenic isolates in a random distribution. The obtained results in this study showed that RAPD profiling is a promising and efficient tool to determine intra-specific genetic variation among A. flavus populations from hospital environments. A. flavus isolates, either toxigenic or non-toxigenic, should be considered as potential threats for hospitalized patients due to their obvious role in the etiology of nosocomial aspergillosis.

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Section: Introductionmentioning

confidence: 99%

A survey on distribution and toxigenicity of Aspergillus flavus from indoor and outdoor hospital environments

et al. 2011

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Fungal biodiversity in the air of Turin

Airaudi

Marchisio

1996

Mycopathologia

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The qualitative fungal composition of Turin's atmospheric environment was surveyed, carrying out a twelve-month study and collecting with a single stage volumetric sieve sampler on Dermasel agar supplemented with 0.4 g l-1 cycloheximide and 0.05 g l-1 chloramphenicol. We isolated 165 species and 2 varieties of mesophilic fungi from 58 genera and 26 thermotolerant species from 12 genera. Penicillium, Aspergillus, Acremonium, Chrysosporium, Scopulariopsis, Malbranchea, Paecilomyces, Phialophora and Cladosporium were in sequence the genera most rich in mesophilic species; Aspergillus, Penicillium, Chrysosporium and Scopulariopsis the most rich in thermotolerant species. Many of the species isolated are rarely or never recorded in the atmospheric environment. Cycloheximide can thus be said to select among airborne fungi, giving a characteristic picture.

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A preliminary survey of cycloheximide-resistant airborne fungi in Turin, Italy

et al. 1993

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Numbers and type of the cycloheximide-resistant part of the aerially transmitted mycoflora of Turin were studied. Samples from three areas characterized by differing human usage were taken in the first week of March. During each sampling, 12 m3 of air were aspirated, using an one-stage volumetric sieve sampler. Fifty-two mesophilic species and eight thermotolerant were isolated. Propagule load varied from 2.92 to 120.31 cfu m-3. The following species appear not to have been reported previously from air samples: Ascotricha bosei, Blastobotrys navarrensis, Cryptendoxyla hypophloia, Chrysosporium an. gymnoascus demonbreunii, Ophiostoma piceae, Penicillium vulpinum, Phialophora mustea, Rhinocladiella pedrosoi, Scopulariopsis croci, S. komngii and Sesquiallium candelabrum. A significant number of potential opportunistic pathogens was isolated.

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Preliminary survey of airborne fungal propagules in Turin, Italy

Cited by 17 publications

References 26 publications

A survey on distribution and toxigenicity of Aspergillus flavus from indoor and outdoor hospital environments

A survey on distribution and toxigenicity of Aspergillus flavus from indoor and outdoor hospital environments

Fungal biodiversity in the air of Turin

A preliminary survey of cycloheximide-resistant airborne fungi in Turin, Italy

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