Preliminary study on the protective effect of vitamin C on monosodium glutamate-induced hepatotoxicity in rats

Waiz, Syma Ashraf; Raies-ul-Haq, Mohammad; Waiz, Hina Ashraf; Gupta, Suman; Pathak, Anupam

doi:10.1007/s00580-014-2033-4

Cited by 8 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vitamin C (ascorbic acid) is a water soluble vitamin so this allowing it to be a good scavenger for aqueous radicals that destroy the membrane lipids. It has a protective role in most of the cytotoxicity [10] . It is considered the cheapest antioxidant to be used so we choose it in this study to evaluate its efficacy and mechanism to ameliorate renal MSG toxicity.…”

Section: Introductionmentioning

confidence: 99%

The possible protective effect of vitamin C on monosodium glutamate induced renal toxicity in male albino rats

Ragab

2018

Egyptian Journal of Histology

View full text Add to dashboard Cite

Introduction: Monosodium glutamate (MSG) is widely used as a food additive to improve the taste of food. Despite its taste stimulation and appetite enhancement, some reports indicated that MSG is toxic and induce an oxidative stress. Vitamin C (ascorbic acid) is a natural antioxidant that prevents the excess production of free radicals. Aim of the work: Our aim was to study the toxicological effect of MSG on the renal cortex of adult male albino rats and to evaluate the possible role of ascorbic acid as a protective agent. Materials and methods: Forty adult albino rats were divided equally into four groups. Group I was the control group; group II received ascorbic acid intraperitonial injection (500 mg/kg/day) for 4 weeks; group III rats were intraperitonial injected with monosodium glutamate (4mg/kg/day) for 4 weeks; and in group IV, rats were injected intraperitonially with ascorbic acid (500 mg/kg/day) for one week then followed by 4 weeks treated with monosodium glutamate and ascorbic acid as the same previous dose. At the end of the experiment, specimens from the kidney were taken and prepared for HandE, immunohistochemical stain and electron microscopic studies. Results: MSG induced degenerative changes in renal tubules as destruction in epithelial cell, loss of the brush border, exfoliated cellular debris in lumen of some tubules. Interstitial cells infiltration and blood vessels congestion were also noticed. There were signs of apoptosis as well as significant increase in caspase 3 antibodies expression. These changes were ameliorated by protective using of the ascorbic acid. Conclusion: MSG caused an apparent kidney injury on the histological, immumohistochemical and ultrastructure level. The ascorbic acid can ameliorate these effects.

show abstract

Section: Introductionmentioning

confidence: 99%

The possible protective effect of vitamin C on monosodium glutamate induced renal toxicity in male albino rats

Ragab

2018

Egyptian Journal of Histology

View full text Add to dashboard Cite

show abstract

“…VC is a good scavenger for aqueous radicals that destroy the membrane lipids. It has a protective role in most of the cytotoxicity ( Waiz et al, 2015 ). The mechanism by which VC reduces BLD-induced hepatic dysfunction is based on the fact that VC can reduce oxidative damage by reducing lipid peroxidation and modifying the antioxidant protection system or denoting free radical electrons and calm down their reactivity.…”

Section: Discussionmentioning

confidence: 99%

Boldenone Undecylenate-Mediated Hepatorenal Impairment by Oxidative Damage and Dysregulation of Heat Shock Protein 90 and Androgen Receptors Expressions: Vitamin C Preventive Role

Behairy¹,

Mohamed²,

Ebraheim³

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Boldenone Undecylenate (BLD) is a synthetic derivative of testosterone and a widely used anabolic androgenic steroid. The health risk of BLD use as a pharmaceutical or dietary supplement is still underestimated and under-reported. Vitamin C (VC) has been recognized as an antioxidant with prominent hepatorenal protective effects. This study investigated the possible preventive activity of VC against BLD-induced hepatorenal damage. Forty adult male Wistar rats were classified into five groups: control, vehicle control, VC (orally given 120 mg/kg b. wt./day), BLD (intramuscularly injected 5 mg/kg b. wt./week), and BLD + VC-treated groups. The experiment continued for eight weeks. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. Serum contents of total protein (TP), albumin (ALB), globulin, total cholesterol (TC), triglycerides (TG), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), and very-low-density lipoprotein–cholesterol (VLDL-C) were also assayed. Urea, creatinine, and uric acid levels were determined together with sodium and potassium electrolytes measuring. Moreover, oxidative stress indicators including reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GSR) as well as malondialdehyde (MDA) levels were measured in both hepatic and renal tissues. Corresponding histological examination of renal and hepatic tissues was conducted. Besides, immunohistochemical evaluations for androgen receptors protein (AR) and heat shock protein 90 (Hsp 90) expressions were performed. BLD caused significant rises in serum ALT, AST, TP, ALB, TC, TG, LDL-C, VLDL-C, urea, creatinine, uric acid, potassium, and MDA levels. Further, BLD-injected rats showed significant declines in the serum levels of HDL-C, sodium, GSH, GPx, GST, and GSR. Besides, distinct histopathological perturbations were detected in renal and hepatic tissues of BLD-injected rats. AR and Hsp 90 immunoexpression were increased in hepatic and renal tissues. In contrast, VC significantly reversed the BLD-induced hepatorenal damage in co-treated rats but not ameliorated AR protein overexpression. VC could be an efficient preventive supplement for mitigating BLD-induced hepatorenal damage, possibly via controlling oxidative stress events.

show abstract

“…C, is a potent scavenger of aqueous radicals that degrade membrane lipids. As it is thought to be the first line of defense against ROS by peroxide reduction, ROS scavenging, repair of peroxidized cellular membranes, and iron sequestration, it has a well-known antioxidant protective role in the majority of the toxicities [19]. Globally, Vit.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Vitamin C and E Against Monosodium Glutamate Induced Histological Changes in The Epididymis of Adult Male Albino Rats

QASIM

Waheed

2023

Egyptian Journal of Veterinary Sciences

View full text Add to dashboard Cite

T HE purpose of the current study was to investigate the possibility of vitamin (Vit.) C and E in protecting rat epididymis from MSG-induced histological changes. Twenty-five adult male albino rats divided into five groups (n=5): control, MSG; MSG+Vit.C.; MSG+Vit.E. and MSG +Vit. C+Vit. E. This treatment lasted for forty days. The histological examinations include use of H&E stain and Masson trichrome and histochemical (Periodic Acid Schiff reaction (PAS)). The present results showed that, all groups' B.W. after treatment was significantly higher than that before treatment. However, after treatment, MSG, and MSG+Vit. E caused a high significant decrease in B.W., respectively. There were no appreciable variations in the epididymis weight between the groups MSG+Vit. E and MSG+Vit. C+Vit. E. Histological investigations, indicated none of the treated groups significantly altered the bulk of the epididymis tissues, except for some histological alteration; however, the MSG+Vit.C+Vit.E group showed few effects. These alterations include blood vessel congestion, inflammatory cell infiltration, giant cell formation, expansion of interstitial spaces, hyperplasia and vacuolation of epithelial tubule lining, and hydropic degeneration. MSG show marked deposition of collagen fibers in the capsule, epididymal tubules, interstitial space and basal lamina. While remaining groups indicated the presence of the moderate amount of collagen fibers. Basal lamina and interstitial cells in groups of the MSG; MSG+Vit.C. and MSG+Vit.E. ; exhibited a strong PAS response. Whereas, the MSG+ Vit.C+ Vit.E group had moderate PAS reaction. In conclusion, combining Vit. C and Vit. E with MSG may lessen its toxicity. It is imperative to reevaluate the use of MSG as a flavor enhancer.

show abstract

Preliminary study on the protective effect of vitamin C on monosodium glutamate-induced hepatotoxicity in rats

Cited by 8 publications

References 22 publications

The possible protective effect of vitamin C on monosodium glutamate induced renal toxicity in male albino rats

The possible protective effect of vitamin C on monosodium glutamate induced renal toxicity in male albino rats

Boldenone Undecylenate-Mediated Hepatorenal Impairment by Oxidative Damage and Dysregulation of Heat Shock Protein 90 and Androgen Receptors Expressions: Vitamin C Preventive Role

Protective Effects of Vitamin C and E Against Monosodium Glutamate Induced Histological Changes in The Epididymis of Adult Male Albino Rats

Contact Info

Product

Resources

About