Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia

Dudek, Katarzyna; Bednarek, D.; Ayling, Roger D.; Kycko, Anna; Reichert, M.

doi:10.1186/s12917-019-2122-3

Cited by 15 publications

(19 citation statements)

References 16 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Infection efficacy in the experimental calves was confirmed by clinical, post mortem and histopathologic observations and the results of immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA) analyses for M. bovis antigen were described previously by Dudek et al. [12]. Following the calf infection with M. bovis, extensive caseous necrosis and lobular consolidation were observed.…”

Section: Resultsmentioning

confidence: 67%

“…The M. bovis antigen was detected in epithelial cells of bronchioli in the lungs of all experimental calves as opposed to the controls, which were negative. All detailed post mortem results were previously described by Dudek et al [12].…”

Section: Resultsmentioning

confidence: 99%

“…The study was performed on 10 four-week-old, clinically healthy female calves housed in the institute's vivarium. Before the proper study, the nasal swabs and blood samples were collected from the calves and examined for Mycoplasma bovis and other respiratory pathogens detection which was described previously by Dudek et al [12]. After a three-week adaptive period, the calves were divided into two groups: experimental (n = 6) and control (n = 4).…”

Section: Animalsmentioning

confidence: 99%

“…All detailed information about the animals and methods used for confirmation of the infection efficacy was described previously by Dudek et al [12].…”

Section: Animalsmentioning

confidence: 99%

“…Instead, the control animals were administered with sterile PBS. It was described previously in detail by Dudek et al [12].…”

Section: Calf Challengementioning

confidence: 99%

See 4 more Smart Citations

Analysis of the Leukocyte Response in Calves Suffered from Mycoplasma bovis Pneumonia

et al. 2020

Self Cite

View full text Add to dashboard Cite

Mycoplasma bovis is known to be a cause of chronic pneumonia in cattle. To date, the disease pathomechanism has not been fully elucidated. Leukocytes play a key role in host antimicrobial defense mechanisms. Many in vitro studies of the effect of Mycoplasma bovis (M. bovis) on leukocytes have been performed, but it is difficult to apply these results to in vivo conditions. Additionally, only a few studies on a local immune response in M. bovis pneumonia have been undertaken. In this study, the experimental calf-infection model was used to determine the effect of field M. bovis strains on changes of the peripheral blood leukocyte response, including phagocytic activity and oxygen metabolism by cytometry analyses. An additional aim was to evaluate the lung local immunity of the experimentally infected calves using immunohistochemical staining. The general stimulation of phagocytic and killing activity of peripheral blood leukocytes in response to the M. bovis infection points to upregulation of cellular antimicrobial mechanisms. The local immune response in the infected lungs was characterized by the T- and B-cell stimulation, however, most seen in the increased T lymphocyte response. Post-infection, strong expression of the antigen-presenting cells and phagocytes also confirmed the activation of lung local immunity. In this study—despite the stimulation—both the peripheral and local cellular antimicrobial mechanisms seem to appear ineffective in eliminating M. bovis from the host and preventing the specific lung lesions, indicating an ability of the pathogen to avoid the host immune response in the M. bovis pneumonia.

show abstract

Section: Resultsmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

“…All detailed information about the animals and methods used for confirmation of the infection efficacy was described previously by Dudek et al [12].…”

Section: Animalsmentioning

confidence: 99%

“…Instead, the control animals were administered with sterile PBS. It was described previously in detail by Dudek et al [12].…”

Section: Calf Challengementioning

confidence: 99%

See 3 more Smart Citations

Analysis of the Leukocyte Response in Calves Suffered from Mycoplasma bovis Pneumonia

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

Design, docking, synthesis, and characterization of novel N'(2-phenoxyacetyl) nicotinohydrazide and N'(2-phenoxyacetyl)isonicotinohydrazide derivatives as anti-inflammatory and analgesic agents

Al-Ostoot

Vivek³

2022

Journal of Molecular Structure

View full text Add to dashboard Cite

The effect of pegbovigrastim administration on the nonspecific immunity of calves

Dudek,

Szacawa,

Bednarek

2023

Veterinary Internal Medicne

Self Cite

View full text Add to dashboard Cite

BackgroundPrevention of diseases in the early rearing of calves is important, particularly because disease occurrence most often requires antimicrobial administration but reduction of their use in animals is a priority. Pegbovigrastim is known for its use as an immunoregulator in cows and heifers, but the effect of its administration on calves has not been fully investigated.ObjectivesInvestigate whether administration of pegbovigrastim effectively stimulates nonspecific immunity in healthy calves.AnimalsEleven clinically healthy 5‐week‐old calves.MethodsProspective observational study. Calves were randomly allocated to an experimental or control groups to receive pegbovigrastim or the same volume of phosphate‐buffered saline twice over a 7‐day period. To evaluate nonspecific immunity, the numbers of total leukocytes and cells in the appropriate cell fractions were determined. Cytometric analyses were carried out to identify cells expressing CD11b and to evaluate the phagocytic and oxidative burst activities of granulocytes and monocytes. Myeloperoxidase (MPO) and selected cytokines were assayed using ELISA.ResultsPegbovigrastim significantly increased the number of total leukocytes and of cells in all of the examined subsets (P < .05). The phagocytic activity of leukocytes expressed as mean fluorescence intensity was significantly potentiated after pegbovigrastim administration (P < .05). The cytokine response was modulated by pegbovigrastim administration toward anti‐inflammatory activity.Conclusions and Clinical ImportancePegbovigrastim effectively stimulated nonspecific immunity in clinically healthy calves, which in the long term could make the prevention of diseases during early rearing possible by strengthening the immune defense mechanisms of the host.

show abstract

Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia

Cited by 15 publications

References 16 publications

Analysis of the Leukocyte Response in Calves Suffered from Mycoplasma bovis Pneumonia

Analysis of the Leukocyte Response in Calves Suffered from Mycoplasma bovis Pneumonia

Design, docking, synthesis, and characterization of novel N'(2-phenoxyacetyl) nicotinohydrazide and N'(2-phenoxyacetyl)isonicotinohydrazide derivatives as anti-inflammatory and analgesic agents

The effect of pegbovigrastim administration on the nonspecific immunity of calves

Contact Info

Product

Resources

About