Preliminary study on the effects of heavy metals on the growth and some antioxidant enzymes in <i>Arthrospira platensis</i>‐M2 strain

Önem, Burçin; Doğru, Ali; Sevi̇ndi̇k, Tuğba Ongun; Tunca, Hatice

doi:10.1111/pre.12202

Cited by 11 publications

(8 citation statements)

References 68 publications

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“…By adjusting antioxidant enzymes, many attempts have been undertaken to increase the salt tolerance of plants (Hu et al 2012 ; Jebara et al 2005 ; Mallik et al 2011 ; Mao et al 2020 ). The induction of the antioxidant enzyme activities of Cyanophyta and Chlorophyta under several environmental conditions has been reported in the literature (Ajitha et al 2019 ; Ismaiel and Said 2018 ; Önem et al 2018 ; Ugya et al 2020 ).…”

Section: Introductionmentioning

confidence: 83%

Antioxidant enzymes of Pseudochlorella pringsheimii under two stressors: variation of SOD Isoforms activity

Ismaiel

Piercey‐Normore

2023

J Plant Res

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Algae are always facing the challenge of exposure to different stress conditions, therefore raising challenges of adaptation for survival. In this context, the growth and the antioxidant enzymes of the green stress-tolerant alga Pseudochlorella pringsheimii were investigated under two environmental stresses viz. iron and salinity. The number of algal cells was moderately increased by iron treatment in the range of 0.025–0.09 mM of iron, yet, the number of cells decreased at high iron concentrations (0.18 to 0.7 mM Fe). Furthermore, the different NaCl concentrations (8.5–136.0 mM) had an inhibitory effect on the algal cell number, compared to the control.The superoxide dismutase (SOD) showed three isoforms namely; Mn, Fe, and Cu/Zn SOD. The in gel and in vitro (tube-test) activities of FeSOD were higher compared with the other SOD isoforms. The activity of total SOD and its isoforms increased significantly by the different concentrations of Fe and non-significantly by NaCl. The maximum SOD activity was recorded at 0.7 mM Fe (67.9% above control). The relative expression of FeSOD was high under iron and NaCl at 8.5 and 34 mM, respectively. However, FeSOD expression was reduced at the highest NaCl tested concentration (136 mM). In addition, the antioxidant enzyme activity of catalase (CAT) and peroxidase (POD) were accelerated by increasing iron and salinity stress which indicates the essential role of these enzymes under stress. The correlation between the investigated parameters was also investigated. A highly significant positive correlation between the activity of total SOD and its isoforms, and with the relative expression of FeSOD was observed.

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Section: Introductionmentioning

confidence: 83%

Antioxidant enzymes of Pseudochlorella pringsheimii under two stressors: variation of SOD Isoforms activity

Ismaiel

Piercey‐Normore

2023

J Plant Res

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“…The samples were centrifugated at 14.000 rpm and 4 °C for 20 min. After treatment, SOD activity was measured as described in Önem et al [ 34 ]. The standard graphic was prepared, and it was used to calculate the quantity of SOD activity as unit mg −1 protein.…”

Section: Methodsmentioning

confidence: 99%

“…The estimation of the ascorbate oxidation decreasing rate at 290 nm was utilized as principle. Önem et al [ 34 ] were perfomed for extraction and determination of the enzyme activity. The initial rate of the reaction using the extinction coefficient of ascorbate (E = 2.8 mM cm −1 at 290 nm) was utilized to calculate enzyme activity.…”

Section: Methodsmentioning

confidence: 99%

“…1.5 mL buffer was prepared with 100 mM K 2 HPO 4 buffer (pH 7.0), 1 mM Na 2 EDTA and 2% PVP for homogenization. The GR activity was measured as described in Önem et al [ 34 ]. The initial rate of the reaction after subtracting the non-enzymatic oxidation using the extinction coefficient of NADPH (E = 6.2 mM cm −1 at 340 nm) was utilized to calculate the enzyme activity.…”

Section: Methodsmentioning

confidence: 99%

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Algaecidal and oxidative effects of metal-free phthalocyanine beta tetra-substituted with sodium 2-mercaptoethanesulfonate

TUNCA¹,

ÖZPINAR²,

Tekbaba³

et al. 2022

Turkish Journal of Chemistry

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In this study, a water-soluble metal-free phthalocyanine (SPC) containing sodium 2-mercaptoethanesulfonate substituents at the peripheral positions was used to investigate the algaecidal properties and oxidative effects on the growth of two microalgal species, Arthrospira platensis and Chlorella vulgaris . Although OD at 560 nm and chlorophyll- a content were decreased in Arthrospira platensis during 7 days depending on dose and time, increases in both OD at 750 and chlorophyll- a content at 8 ppb (parts per billion) concentration on the 7th day were observed in Chlorella vulgaris . However, total SOD (superoxide dismutase) and GR (glutathione reductase) enzyme activity of A. platensis cultures did not display any alteration in all concentrations, SOD activity displayed an increase significantly at 2 ppb concentration, and GR activity showed increases at 1, 2, and 4 ppb concentrations in C. vulgaris application. In A. platensis application, APX (ascorbate peroxidase) activity decreased at 0.50 ppb, 1 ppb, and 1.5 ppb concentrations. In addition, C. vulgaris application showed decreases at all concentrations. When MDA content increased at all concentrations, the H 2 O 2 content increased only at significatly 0.125 ppb concentration in A. platensis cultures. Both MDA (malondialdehyde) and H 2 O 2 (hydrogen peroxide) content of C. vulgaris cultures showed a statistically significant decrease at all concentrations compared to control. Free proline decreased at 0.25 ppb, 0.50 ppb, 1 ppb, and 1.5 ppb concentrations in A. platensis application, and it decreased at all the concentrations of C. vulgaris application. It concluded that this compound has inhibition effects on A. platensis , but it supports growth in C. vulgaris . Therefore, this synthesized phthalocyanine compound (SPC) should be consumed carefully, and the contamination to aquatic ecosystems should be prevented.

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“…Among the founts of natural compounds, microalgae biomass represents a good source of antioxidants, as microalgae are capable of high production of these metabolites (phenolic compounds, carotenoids, polyunsaturated fatty acids) under stress conditions, making increasingly important to focus attention on studying the physiology of these microorganisms [1][2][3]. Most importantly, microalgae, respect to higher plants, which can be also rich in antioxidant compounds, have a higher growth rate and can be cultivated in photo-bioreactors, in extreme environments, without competing with arable lands.…”

Section: Introductionmentioning

confidence: 99%

Impact of Light Stress on the Synthesis of Both Antioxidants Polyphenols and Carotenoids, as Fast Photoprotective Response in Chlamydomonas reinhardtii: New Prospective for Biotechnological Potential of This Microalga

2021

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The aim of this study was to investigate the potential role of the microalga Chlamydomonas reinhardtii as an antioxidant source of enriched biomass. This microalga is a model organism deeply investigated for physiological studies, particularly considering carotenoid synthesis in response to stress, to counteract the effects of the formation of free radicals. Less attention has been paid to the profile characterization of other antioxidant compounds, such as polyphenols, which can be synthesized, concomitantly with carotenoids, under photooxidative stress, especially high light. The cultures of C. reinhardtii were exposed to three different light intensities, 70, 800 and 1500 µmoles photons m−2 s−1. The increasing light intensity symmetrically induced the increasing accumulation of both carotenoids and phenolic compounds. The results showed that exposure to high light intensities caused the accumulation of electrons in the electron transport chain, with a reduction in photosynthetic activity. In the same cultures, high light intensity induced the strong increment of polyphenols such as gallic, chlorogenic and coumaric acids, which resulted 6.2-fold, 4-fold and 3.7-fold higher, respectively, than in cells exposed to the lowest intensities. As expected, at the highest light intensity, the strong induction of the xanthophyll cycle and the largest increment of loroxanthin, lutein, α-carotene and ß-carotene could be detected. Antioxidant properties doubled with respect to the initial time, both in acetone and methanol cellular extracts of these cultures, revealing a new potential role for biotechnological application of this microalga.

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Preliminary study on the effects of heavy metals on the growth and some antioxidant enzymes in Arthrospira platensis‐M2 strain

Cited by 11 publications

References 68 publications

Antioxidant enzymes of Pseudochlorella pringsheimii under two stressors: variation of SOD Isoforms activity

Antioxidant enzymes of Pseudochlorella pringsheimii under two stressors: variation of SOD Isoforms activity

Algaecidal and oxidative effects of metal-free phthalocyanine beta tetra-substituted with sodium 2-mercaptoethanesulfonate

Impact of Light Stress on the Synthesis of Both Antioxidants Polyphenols and Carotenoids, as Fast Photoprotective Response in Chlamydomonas reinhardtii: New Prospective for Biotechnological Potential of This Microalga

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