Preliminary study of the influence of thyroxine, temperature, and sex on oxygen uptake by tissues from the spadefoot toad Scaphiopus bombifrons

Packard, Gary C.; Packard, Mary J.

doi:10.1016/0016-6480(73)90084-1

Cited by 12 publications

(1 citation statement)

References 12 publications

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“…The activity of mitochondrial a-glycerophosphate dehydrogenase (a-GPD) (E.C.I.I.99.5) has been widely used as an index of thyroid hormone effect {Jane and McMurry 1983;Oppenheimer 1975). Although there are informations of relative unresponsiveness of the adult amphibia to thyroid hor-mone (Gallon 1983;1985), the thyroid gland of such unresponsive adult frog can synthesize thyroxine (T4) and triiodothyronine (T3) {Leloup and Fontaine 1960; Ceusters, Darras and Kuhn 1978), and increase in oxygen consumption has been found after T4 administration to frogs and toads acclimated above 20 °C {McNabb 1969;May and Packer 1976) and also in isolated liver slices from these animals (Packard and Packard 1973;1975). In an earlier report from our laboratory, the changes in protein and nucleic acid metabolism in adult toad liver and muscle after T4 treatment have been shown (Ray and Medda 1974), and recently we have also reported the responsiveness of adult toad to T3 and T4 with respect to the increase in the activity of NADP-dependent cytosolic malic dehydrogenase in liver and muscle, suggesting the possible role of thyroid hormone in the metabolism of adult toad (Dey, Dasmahapatra, Ray and Medda 1989).…”

Section: Introductionmentioning

confidence: 99%

Effect of Triiodothyronine and Thyroxine on Mitochondrial α-Glycerophosphate Dehydrogenase Activity and Mitochondrial Protein Content of Liver, Muscle and Brain of Toad,Bufo melanostictus

Dey¹,

Mandal²

1990

Horm Metab Res

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The responsiveness of the adult toad to triiodothyronine (T3) and thyroxine (T4) was studied by measuring the mitochondrial alpha-glycerophosphate dehydrogenase activity and mitochondrial protein content of liver, muscle and brain of toad. Both T3 and T4 increased the alpha-GPD activity and mitochondrial protein content of liver and muscle of toad. The extent of increase in the alpha-GPD activity and mitochondrial protein content were more pronounced with T3 than with T4. Further that the muscle exhibited more alpha-GPD activity than liver, whenever liver showed greater mitochondrial protein content than that of muscle. Brain showed no significant change in the alpha-GPD activity and mitochondrial protein content. Injections of cycloheximide showed inhibition of T3 induced changes in liver and muscle. Injection of propylthiouracil also counteracted the T4 induced effects of liver and muscle.

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Section: Introductionmentioning

confidence: 99%

Effect of Triiodothyronine and Thyroxine on Mitochondrial α-Glycerophosphate Dehydrogenase Activity and Mitochondrial Protein Content of Liver, Muscle and Brain of Toad,Bufo melanostictus

Dey¹,

Mandal²

1990

Horm Metab Res

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The influence of thyroxine and acclimation temperature on glycogen reserves of the frog Rana pipiens

Packard

Randall

1975

J. Exp. Zool.

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Leopard frogs were given daily injections of Na-l-thyroxine (2.0 mug/10 g body weight) for seven days, and control animals were given daily injections of the vehicle. During the period of treatment, half of the frogs were acclimated, in darkness and without food, at a constant temperature of 15 degrees C, whereas the remainder of the animals were acclimated at 25 degrees C. At the end of the seventh day, the frogs were killed by decapitation, and the glycogen concentration in samples of liver tissue and skeletal muscle was determined spectrophotometrically. Total hepatic glycogen of each frog subsequently was calculated from data on glycogen concentration and liver weight. Treatment of leopard frogs with thyroxine had no apparent effect on hepatic glycogen reserves of animals acclimated at 15 degrees C, but there was a striking reduction in glycogen content of livers from hyperthyroid frogs held at 25 degrees C. Mobilization of hepatic glycogen in thyroxine-treated animals held at 25 degrees C presumably was secondary to a general stimulation of oxidative metabolism. Treatment with thyroxine had no effect on the concentration of glycogen in skeletal muscle of leopard frogs held at either of the acclimation temperatures used in this study.

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The influence of thyroxine on oxygen consumption of tissues from the frog Rana pipiens

Packard

Stiverson

1974

General and Comparative Endocrinology

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Preliminary study of the influence of thyroxine, temperature, and sex on oxygen uptake by tissues from the spadefoot toad Scaphiopus bombifrons

Cited by 12 publications

References 12 publications

Effect of Triiodothyronine and Thyroxine on Mitochondrial α-Glycerophosphate Dehydrogenase Activity and Mitochondrial Protein Content of Liver, Muscle and Brain of Toad,Bufo melanostictus

Effect of Triiodothyronine and Thyroxine on Mitochondrial α-Glycerophosphate Dehydrogenase Activity and Mitochondrial Protein Content of Liver, Muscle and Brain of Toad,Bufo melanostictus

The influence of thyroxine and acclimation temperature on glycogen reserves of the frog Rana pipiens

The influence of thyroxine on oxygen consumption of tissues from the frog Rana pipiens

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