Preliminary Study of Terpenes from the Leaves of Cyclocarya paliurus

Liang, Lin-Fu; Du, Shan-Nan; Huang, Kaiyu; Li, Kun; Fan, Yue-Yang

doi:10.1007/s10600-018-2355-6

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…The results revealed that the 1 H and 13 C NMR chemical shifts (Additional file 1 : Figs. S10, S11) were consistent with those of the germacrene D described in a previous report [ 28 ]. The specific rotation value of the AcTPS1 major product was -30.667 (c = 0.25, CHCl 3 ); the specific rotation value of the (–)-germacrene D standard was -44.871 (c = 0.26, CHCl 3 ) (Additional file 1 : Table S5).…”

Section: Resultssupporting

confidence: 91%

Identification of the sesquiterpene synthase AcTPS1 and high production of (–)-germacrene D in metabolically engineered Saccharomyces cerevisiae

Li¹,

Chen²,

Wan³

et al. 2022

Microb Cell Fact

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Background The sesquiterpene germacrene D is a highly promising product due to its wide variety of insecticidal activities and ability to serve as a precursor for many other sesquiterpenes. Biosynthesis of high value compounds through genome mining for synthases and metabolic engineering of microbial factories, especially Saccharomyces cerevisiae, has been proven to be an effective strategy. However, there have been no studies on the de novo synthesis of germacrene D from carbon sources in microbes. Hence, the construction of the S. cerevisiae cell factory to achieve high production of germacrene D is highly desirable. Results We identified five putative sesquiterpene synthases (AcTPS1 to AcTPS5) from Acremonium chrysogenum and the major product of AcTPS1 characterized by in vivo, in vitro reaction and NMR detection was revealed to be (–)-germacrene D. After systematically comparing twenty-one germacrene D synthases, AcTPS1 was found to generate the highest amount of (–)-germacrene D and was integrated into the terpene precursor-enhancing yeast strain, achieving 376.2 mg/L of (–)-germacrene D. Iterative engineering was performed to improve the production of (–)-germacrene D, including increasing the copy numbers of AcTPS1, tHMG1 and ERG20, and downregulating or knocking out other inhibitory factors (such as erg9, rox1, dpp1). Finally, the optimal strain LSc81 achieved 1.94 g/L (–)-germacrene D in shake-flask fermentation and 7.9 g/L (–)-germacrene D in a 5-L bioreactor, which is the highest reported (–)-germacrene D titer achieved to date. Conclusion We successfully achieved high production of (–)-germacrene D in S. cerevisiae through terpene synthase mining and metabolic engineering, providing an impressive example of microbial overproduction of high-value compounds.

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Section: Resultssupporting

confidence: 91%

Identification of the sesquiterpene synthase AcTPS1 and high production of (–)-germacrene D in metabolically engineered Saccharomyces cerevisiae

Li¹,

Chen²,

Wan³

et al. 2022

Microb Cell Fact

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“…developed enzyme‐triggered self‐assembly of small molecules into nanostructures for imaging applications, which is based on a biocompatible condensation reaction of 1,2‐aminothiol and 2‐cyanobenzothiazole (CBT) derived from the last step of d ‐luciferin synthesis . Under the control of pH, disulfide reduction, or protease cleavage, the probe was activated leading to condensation polymerization of monomers and self‐assembly into nanostructures in living cells ( Figure a) . Due to the existence of furin (a protease) primarily localized in the Golgi bodies, the fluorescence signal was observed near Golgi bodies in furin‐expressing cell lines, demonstrating the controlled and localized imaging of furin activity.…”

Section: Nanoparticles and Nanoaggregatesmentioning

confidence: 99%

Section: Nanoparticles and Nanoaggregatesmentioning

confidence: 99%

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Molecular Self‐Assembly Constructed in Physiological Conditions for Cancer Diagnosis and Therapy

Qiao

Wang

2018

Advanced Therapeutics

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For the purpose of cancer theranostics, the performance and potential biosafety of drug/imaging agents remain major challenges. Although passive and active targeting strategies have been reported for effectively enhanced bioimaging or drug delivery performance, researchers are still looking for emerging methods for better targeting, accumulation, and penetration of solid tumors. The strategy for constructing supramolecular self-assemblies in biologically relevant conditions may partially overcome these challenges. Regarding the biomedical applications, supramolecular entities with naturally dynamic and modular properties, are capable of constructing structurally and functionally diversified materials and meeting the requirements of biological complexity. Modules and integrated systems enable response to bio-environment by the modification of their constitution through component exchange or reorganization. In this review, the authors summarize the self-assembled nanosystems (nanoparticles, nanoaggregates, micelles, vesicles, nanofibers, hydrogels, etc.) in physiological/pathological environments and their applications in cancer theranostics. The authors focus on chemical structures, stimuli-responsive bonds, and self-assembly property of nanomaterials, which may offer a guide for the optimal design of self-assembled nanomaterials in clinical cancer treatment and imaging.

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“…Furin: Furin is one of central regulators of tumor progression/ metastasis and a proven drug target in vivo. [37] The detection of furin and furin-like activity in living subjects is of significant interest. For example, Gambhir and co-workers designed enzymesensitive oligomerizable reactive PA probe (ESOR-PA01) for the detection of furin-like activity both in vitro and in vivo.…”

Section: Enzyme Detectionsmentioning

confidence: 99%

Photoacoustic Probes for Molecular Detection: Recent Advances and Perspectives

Zeng

Lin

et al. 2018

Small

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Photoacoustic (PA) imaging (PAI) is a noninvasive and nonionizing biomedical imaging modality that combines the advantages of optical imaging and ultrasound imaging. Based on PAI, photoacoustic detection (PAD) is an emerging approach that is involved with the interaction between PA probes and analytes resulting in the changes of photoacoustic signals for molecular detection with rich contrast, high resolution, and deep tissue penetration. This Review focuses on the recent development of PA probes in PAD. The following contents will be discussed in detail: 1) the construction of PA probes; 2) the applications and mechanisms of PAD to different types of analytes, including microenvironments, small biomolecules, or metal ions; 3) the challenges and perspectives of PA probes in PAD.

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Preliminary Study of Terpenes from the Leaves of Cyclocarya paliurus

Cited by 6 publications

References 15 publications

Identification of the sesquiterpene synthase AcTPS1 and high production of (–)-germacrene D in metabolically engineered Saccharomyces cerevisiae

Identification of the sesquiterpene synthase AcTPS1 and high production of (–)-germacrene D in metabolically engineered Saccharomyces cerevisiae

Molecular Self‐Assembly Constructed in Physiological Conditions for Cancer Diagnosis and Therapy

Photoacoustic Probes for Molecular Detection: Recent Advances and Perspectives

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