Preliminary study of FMO1, FMO5, CYP21, ESR1, PLIN2 and SULT2A1 as candidate gene for compounds related to boar taint

Neuhoff, Christiane; Gunawan, Asep; Farooq, Malik; Çınar, Mehmet Ulaş; Große‐Brinkhaus, Christine; Sahadevan, Sudeep; Frieden, Luc; Tesfaye, Dawit; Tholen, Ernst; Looft, Christian; Schellander, K.; Uddin, Muhammad Jasim

doi:10.1016/j.meatsci.2015.05.025

Cited by 7 publications

(4 citation statements)

References 42 publications

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“…Pig FMO1 has a K m from 0.3 to 10 M in vitro for several organic selenium-containing xenobiotics (6), and both hFMO1 and hFMO3 are capable of catalyzing the formation of pyruvate from selenocysteine (12), consistent with a role in endogenous selenium metabolism. Mammalian FMO5 does not metabolize typical FMO substrates, and may act more like a BVMO (8,13).…”

Section: Substratesmentioning

confidence: 99%

Flavin-containing monooxygenases in aging and disease: Emerging roles for ancient enzymes

Rossner

Kaeberlein

Leiser

2017

Journal of Biological Chemistry

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Edited by F. Peter GuengerichFlavin-containing monooxygenases (FMOs) are primarily studied as xenobiotic metabolizing enzymes with a prominent role in drug metabolism. In contrast, endogenous functions and substrates of FMOs are less well understood. A growing body of recent evidence, however, implicates FMOs in aging, several diseases, and metabolic pathways. The evidence suggests an important role for these well-conserved proteins in multiple processes and raises questions about the endogenous substrate(s) and regulation of FMOs. Here, we present an overview of evidence for FMOs' involvement in aging and disease, discussing the biological context and arguing for increased investigation into the function of these enzymes.

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Section: Substratesmentioning

confidence: 99%

Flavin-containing monooxygenases in aging and disease: Emerging roles for ancient enzymes

Rossner

Kaeberlein

Leiser

2017

Journal of Biological Chemistry

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“…Previous study conducted by Gunawan et al (2013a) reported in animals with different families of cytochrome CYP4A25 that genotype AC had higher value in skatole. Neuhoff et al (2015) reported in animal with different families of cytochrome CYP21 (g.3911T<C) were found that homozygous CC genotype had higher value in skatole. Both genes were detected in Hardy Weinberg Equlibrium (HWE) (P<0.05).…”

Section: Discussionmentioning

confidence: 88%

Association and Expression of CYP2A6 and KIF12 Genes Related to Lamb Flavour and Odour

Listyarini

Jakaria

Uddin

et al. 2018

Trop. Anim. Sci. J.

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Cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6) and kinesin-like protein KIF12 (KIF12) genes are predicted as candidate genes which play important roles in lamb flavour and odour. The aim of this study was to analyse the genotype polymorphism of CYP2A6 and KIF12 genes, to study association and expression of these genes with lamb flavour and odour. Identification of genes polymorphism and associations of CYP2A6 and KIF12 genes were performed using PCR-RFLP method and GLM analysis. The PCR-RFLP products of CYP2A6 and KIF12 were digested by restriction enzyme BsmAI and BfaI, respectively. The expression of CYP2A6 gene was performed using qRT-PCR. The results showed that the CYP2A6 and KIF12 genes were polymorphics. The CYP2A6 gene found to have two genotypes (TT and GT), whereas the KIF12 gene found to have three genotypes (CC, CT, and TT). The CYP2A6 and KIF12 genes were in Hardy Weinberg Equilibrium (HWE). Association analysis showed that CYP2A6 (g.49170107 G>T) was significantly (P<0.05) associated with 3-methylindole (MI) or skatole, while KIF12 (g.9617965 C>T) was not significantly associated with lamb flavour and odour. The GT genotype exhibited a greater 3-methylindole (MI) or skatole than the TT genotype (P<0.05). The mRNA expression analysis showed that CYP2A6 mRNA expression was higher (P<0.01) in animals with the TT genotype. These results will improve the understanding of the functions of the CYP2A6 in lamb flavour and odour, especially in term of 3-methylindole (MI) or skatole compound within the liver and will shed light on CYP2A6 as a candidate in the selection of sheep with low lamb flavour and odour.

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“…This became particularly clear for features that showed a difference only in one group compared to the other three—for example, the elevated blood fumarate levels in PIxGL boars ( Figure 3 ). In the past, gender differences have mainly been discussed regarding boar taint, and differences in fat composition and fatty acid profiles, which affect consumers perception and technical processing to meat products [ 34 , 35 , 36 , 37 ]. Bovo et al, 2015 [ 38 ] applied a large study on mature female and castrated Italian Large White pigs.…”

Section: Discussionmentioning

confidence: 99%

Sexual Dimorphism of Metabolite Profiles in Pigs Depends on the Genetic Background

et al. 2021

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The study aimed to investigate possible systematic effects in the basic underlying variability of individual metabolomic data. In this context, the extent of gender- and genotype-dependent differences reflected in the metabolic composition of three tissues in fattening pigs was determined. The 40 pigs belonged to the genotypes PIx(LWxGL) and PIxGL with gilts and boars, respectively. Blood and tissue samples from M. longissimus dorsi and liver were directly taken at the slaughtering plant and directed to GC × GC qMS metabolite analysis. Differences were observed for various metabolite classes like amino acids, fatty acids, sugars, or organic acids. Gender-specific differences were much more pronounced than genotype-related differences, which could be due to the close genetic relation of the fattening pigs. However, the metabolic dimorphism between gilts and boars was found to be genotype-dependent, and vice versa metabolic differences between genotypes were found to be gender-dependent. Most interestingly, integration into metabolic pathways revealed different patterns for carbon (C) and nitrogen (N) usage in boars and gilts. We suppose a stronger N-recycling and increased energy metabolism in boars, whereas, in gilts, more N is presumably excreted and remaining carbon skeletons channeled into lipogenesis. Associations of metabolites to meat quality factors confirmed the applicability of metabolomics approaches for a better understanding about the impact of drivers (e.g., gender, age, breed) on physiological processes influencing meat quality. Due to the huge complexity of the drivers-traits-network, the derivation of independent biomarkers for meat quality prediction will hardly be possible.

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Preliminary study of FMO1, FMO5, CYP21, ESR1, PLIN2 and SULT2A1 as candidate gene for compounds related to boar taint

Cited by 7 publications

References 42 publications

Flavin-containing monooxygenases in aging and disease: Emerging roles for ancient enzymes

Flavin-containing monooxygenases in aging and disease: Emerging roles for ancient enzymes

Association and Expression of CYP2A6 and KIF12 Genes Related to Lamb Flavour and Odour

Sexual Dimorphism of Metabolite Profiles in Pigs Depends on the Genetic Background

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