Preliminary study of a challenge test to the patients with Japanese cedar pollinosis using an environmental exposure unit

Ito, Kanako; Takato, Tsuyoshi; Yuki, Ayumi; Ichihara, Takahiro; Hyo, Sawako; Kawata, Ryo; Takenaka, Hiroshi; Amano, Kazuyuki; Ishiguro, Takeshi; Ro, Toshitami

doi:10.1016/j.anl.2010.04.001

Cited by 14 publications

(6 citation statements)

References 14 publications

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“…The EAACI Task Force Report indicates that at least 12 AECs are operational globally; comparing their advantages and disadvantages, including dimensions and structures, operating conditions, pollen/particle counting, tested allergens/substances and validation procedures 9 . These chambers have utilized a variety of allergens, with the most frequently used being grass pollen, ragweed, birch pollen, house dust mites and Japanese cedar 20–25 …”

Section: Discussionmentioning

confidence: 99%

“…9 These chambers have utilized a variety of allergens, with the most frequently used being grass pollen, ragweed, birch pollen, house dust mites and Japanese cedar. [20][21][22][23][24][25] Notable validation studies for controlled grass pollen exposure have been conducted in several locations, including the Ottawa Allergen Challenge Theater ACT, 26 Vienna Challenge Chamber VCC, 27 Environmental Exposure Chamber at Aarhus University in Denmark, 28 the Fraunhofer Allergen Challenge Chamber in Hannover, Germany, 29 Global Allergy and Asthma European Network (GA 2 LEN) Mobile Exposure Chamber, 30 and Allergen BioCube®, Massachusetts, USA. 31 Various studies assessing grass pollen allergens used diverse grass types including timothy grass (Phleum pratense), orchard grass (Dactylis glomerata) and rye grass (Lolium Perenne).…”

Section: Reproducibility Of Symptomsmentioning

confidence: 99%

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Clinical validation of grass pollen exposure chamber in patients with allergic rhinitis triggered by timothy grass

Kosowska,

Zemelka‐Wiącek,

Smolińska

et al. 2024

Clin Experimental Allergy

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BackgroundThe fluctuation in concentrations of airborne allergens frequently presents a challenge to assessing the efficacy of allergen immunotherapy (AIT) in ‘field’ studies. Allergen exposure chambers (AECs) are specialized medical installations developed to expose individuals to allergens at defined and consistent concentrations under a controlled environment. The aim of the study was to validate the provocation test with timothy grass pollen as well as to assess its safety in the AEC in patients with allergic rhinitis.MethodsIn the ALLEC® AEC, varying concentrations of timothy grass pollen were dispersed. Allergic symptoms were measured by total nasal symptom score (TNSS), acoustic rhinometry, peak nasal inspiratory flow (PNIF) and nasal discharge volume. Lung function, assessed through peak expiratory flow rate (PEFR) and forced expiratory volume in the first second (FEV1), was used to evaluate safety.ResultsThe consistency of the test was proved by the stability of environmental conditions, including temperature, humidity and CO2 levels, as well as constant concentrations of grass pollen at predetermined levels ranging from 1000 to 10,000 particles per cubic meter (p/m3). Allergic individuals developed symptoms at concentrations of 3000 p/m3 and above, across all measured endpoints. Lung function was not affected throughout all the challenges. The reproducibility of symptoms was confirmed throughout the tests. The concentration of 8000 p/m3 together with a challenge duration of 120 min was found to be optimal.ConclusionThe study demonstrates that the ALLEC® grass pollen exposure chamber provides a reliable and safe method for inducing repeatable symptoms in patients with allergic rhinitis. This approach can be effectively applied for allergy diagnostics and clinical endpoint determination during AIT.

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Section: Discussionmentioning

confidence: 99%

Section: Reproducibility Of Symptomsmentioning

confidence: 99%

Clinical validation of grass pollen exposure chamber in patients with allergic rhinitis triggered by timothy grass

Kosowska,

Zemelka‐Wiącek,

Smolińska

et al. 2024

Clin Experimental Allergy

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“…According to the EAACI Task Force Report, 8 there is over a dozen AEC worldwide. Various allergens have been tested, and the most common are; grass pollen, ragweed pollen, birch pollen, HDM, or Japanese cedar pollen 23–28 . Validation of controlled exposure to HMD‐allergen was performed at: Vienna Challenge Chamber VCC2 and VCC3 (Vienna, Austria), 29 HDM‐environmental exposure unit (EEU) in Kingston Health Sciences Centre‐KGH (Kingston, ON, Canada), 30 Fraunhofer allergen challenge chamber (Hannover, Germany), 31 Biogenics Research Chamber (San Antonio, TX, USA), 32 and environmental exposure chamber (EEC) ALYATEC (Strasbourg, France) 33 .…”

Section: Discussionmentioning

confidence: 99%

“…Various allergens have been tested, and the most common are; grass pollen, ragweed pollen, birch pollen, HDM, or Japanese cedar pollen. 23 , 24 , 25 , 26 , 27 , 28 Validation of controlled exposure to HMD‐allergen was performed at: Vienna Challenge Chamber VCC2 and VCC3 (Vienna, Austria), 29 HDM‐environmental exposure unit (EEU) in Kingston Health Sciences Centre‐KGH (Kingston, ON, Canada), 30 Fraunhofer allergen challenge chamber (Hannover, Germany), 31 Biogenics Research Chamber (San Antonio, TX, USA), 32 and environmental exposure chamber (EEC) ALYATEC (Strasbourg, France). 33 However, these AECs vary in terms of the HDM material source, delivery system, measurements of the distributed particles, and exposure time, making the study comparison very difficult.…”

Section: Discussionmentioning

confidence: 99%

Validated allergen exposure chamber is plausible tool for the assessment of house dust mite‐triggered allergic rhinitis

Zemelka‐Wiącek

Kosowska

Winiarska

et al. 2022

Allergy

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Background: Allergen exposure chamber (AEC) is a clinical facility that allows exposure to allergenic airborne particles in controlled environment. Although AECs offer stable levels of airborne allergens, the validation of symptoms and other endpoints induced by allergen challenge is key for their recommendation as a plausible tool for the assessment of patients, especially in clinical research. This study aimed to demonstrate the reproducibility of defined clinical endpoints after AEC house dust mite (HDM) challenge under optimal conditions in patients with allergic rhinitis (AR). Method: HDM was distributed at different concentrations. The assessment was subjective by the patients: total nasal symptom score (TNSS), visual analog scale (VAS), and objective by the investigator: acoustic rhinometry, peak nasal inspiratory flow (PNIF), and nasal secretion weight. Safety was assessed clinically and by peak expiratory flow rate (PEFR) and forced expiratory volume in the first second (FEV 1 ).Results: Constant environment: temperature, humidity, and carbon dioxide (CO 2 ) concentration were maintained during all challenges. The concentration of HDM on average remained stable within the targeted values: 1000, 3000, 5000, 7000 particles (p)/m 3 . Most symptoms were observed at concentrations 3000 p/m 3 or higher. The symptoms severity and other endpoints results were reproducible. 5000 p/m 3 , and challenge duration of 120 min were found optimal. The procedure was safe with no lung function abnormalities due to challenge. Conclusion:HDM challenge in ALL-MED AEC offers a safe and reliable method for inducing symptoms in AR patients for the use in controlled clinical studies including allergen immunotherapy.

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“…Other EEUs have included the Vienna Challenge Chamber in Austria;5 the Denmark chamber;6 the Fraunhofer Institute Environmental Challenge Chamber in Germany;7 the GA 2 LEN chamber in Germany;8 the OHIO, Wakayama, and Osaka/Chiba units in Japan;9–11 the Environmental Exposure Chamber in Canada;12 allergen Challenge Theatre in Canada;13 and the Atlanta Allergen Exposure Unit,14 the Allergen BioCube,15 and the Biogenics Research Chamber16 in the US. Several EEUs are described by Day et al3 and Ellis et al17…”

Section: Introductionmentioning

confidence: 99%

Technical and clinical validation of an environmental exposure unit for ragweed

Gomes¹,

Lane²,

Angjeli³

et al. 2016

JAA

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BackgroundAllergic rhinitis is a common condition, with ragweed pollen one of the more prevalent aeroallergens. Environmental exposure units such as the Allergen BioCube® are valuable models for clinical allergy studies. A study was conducted to validate the Allergen BioCube for uniform ragweed pollen concentrations and clinically relevant sign and symptom responses to ragweed exposure.MethodsRagweed pollen concentrations were measured on 3 consecutive days in the Allergen BioCube and verified by Rotorod collection and continuous laser particle count measurements. Subjects (N=10) were exposed to ragweed pollen in the BioCube for 3 hours per day for 3 consecutive days. Subjects assessed their nasal itching, sneezing, rhinorrhea, and nasal congestion during each BioCube exposure; total nasal symptom score was computed. Peak nasal inspiratory flow was also assessed during BioCube exposure.ResultsUniform ragweed pollen concentrations were obtained throughout each of the 3-hour testing periods in the Allergen BioCube, both spatially and temporally, at all subject positions, with a low mean standard deviation of 10%. Pronounced increases in mean total nasal symptom scores (6.7±0.94 to 7.6±0.86, last 90 minutes of exposure) occurred for all three BioCube ragweed pollen exposure visits. Mean peak nasal inspiratory flow decreased 24% at 3 hours of BioCube exposure on Day 3. No safety issues of concern occurred in this study.ConclusionThe Allergen BioCube achieved technical and clinical validation for ragweed allergen. Ragweed pollen concentration was uniform both temporally and spatially. Allergic rhinitis signs and symptoms were induced in subjects during exposure to ragweed in the BioCube at clinically meaningful levels for allergy studies.

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Preliminary study of a challenge test to the patients with Japanese cedar pollinosis using an environmental exposure unit

Cited by 14 publications

References 14 publications

Clinical validation of grass pollen exposure chamber in patients with allergic rhinitis triggered by timothy grass

Clinical validation of grass pollen exposure chamber in patients with allergic rhinitis triggered by timothy grass

Validated allergen exposure chamber is plausible tool for the assessment of house dust mite‐triggered allergic rhinitis

Technical and clinical validation of an environmental exposure unit for ragweed

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