Preliminary Studies on Citotoxicity and Genotoxicity Assessment of the PMMA-TiO2 Nanocompozites for Stereolithographic Complete Dentures Manufacturing

Abstract

The aim of the present study was to characterize and preliminary assess the cytotoxicity and genotoxicity of different PMMA-TiO2 nanocomposites suitable to be used for two-parts stereolithographic complete denture manufacturing. In order to improve mechanical and antibacterial characteristics of the materials for 3D printing dentures, two matrix materials consisting in commercially available solutions of poly(methyl methacrylate) (PMMA), referred as dent-PMMA respectively base-PMMA, have been doped with different amount of TiO2 nanoparticles: 0.4, 0.6, 1.0, and 4.0% by weight. The obtained nanocomposites were structural characterized and assessed for antibacterial activity, cytotoxicity and genotoxicity. The newly obtained nanocomposites presented a good inhibitory action against the considered bacterial species-Staphylococcus aureus, good biocompatibility under 4.0% by weight. The results of the preliminary studies on cytotoxicity and genotoxicity assessment recommends the use of 0.4% TiO2 dent-PMMA and base-PMMA for CAD/CAM additive manufacturing of two-parts complete dentures/overdentures.

Structural Investigations on Poly(methyl methacrylate) Various Composites Used for Stereolithographyc Complete Dentures

“…The morphological and structural studies presented in this work offer a valuable confirmation of our previous findings [6,7,18] regarding the improved characteristics of the PMMA-TiO 2 nanocomposite.…”

Eighteen Months Follow-Up with Patient-Centered Outcomes Assessment of Complete Dentures Manufactured Using a Hybrid Nanocomposite and Additive CAD/CAM Protocol

“…The nanoTiO 2 hybrid material proved to be adequate for DLP technology, with excellent biocompatibility [10] in the oral environment and with antibacterial properties against Candida species [5], the results being presented in previous published papers.…”

“…Due to the high demand for rapid, accurate, and resistant material suitable for long-term 3D-printed complete dentures manufacturing, we proposed an improved nanocomposite based on poly(methyl methacrylate) with titanium dioxide nanoparticle inclusions. The newly obtained poly(methyl methacrylate)-TiO 2 matrix allowed a material with proven antimicrobial action [10], smooth surface aspect [11], and better mechanical and thermal characteristics [12] to be obtained, suitable for use with additive technology [5].…”

Poly(methyl metacrylate) Nanocomposites for Two-piece CAD/CAM Solution as an Alternative to Monolithic Removable Prosthesis

“…The complete procedure for obtaining a homogeneous PMMA denture base complies with the preparation method described in detail in another paper [3]. Due to our earlier findings [2,5,6] the optimal amount of the titanium oxide nanoparticles added to the polymeric matrix is 0.4% (w/w).…”