Preliminary studies of PVA/PVP blends incorporated with HAp and β-TCP bone ceramic as template for hard tissue engineering

Abstract

Polyvinylalcohol (PVA) and Polyvinylpyrrolidone (PVP) blend incorporated with hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) is electrospun as nanofibrous composite scaffolds to act as suitable template for bone tissue engineering. Microscopic and spectroscopic characterizations confirm uniform integration of the crystalline calcium phosphate ceramics in the scaffolds. PVA-PVP blends are usually amorphous in nature and addition of phosphate ceramic particles specifically HAp elevates its crystalline behavior which is substantiated by XRD details. Further incorporation of ceramics is confirmed using FT-IR as characteristic PO43- groups for HAp and β-TCP were observed in the distinguished composites. Single glass transition temperature is observed for pure and composite blends indicating the formation of highly miscible blends. Also, addition of these ceramics augments the thermal stability of the blend scaffolds. Biocompatibility of the prepared (PVA: PVP)-HAp and (PVA: PVP)-TCP scaffolds is assessed using MG-63 Osteoblast cell lines in the time interval of 1st, 4th and 7th day. The cell viability percentage for (PVA: PVP)-HAp is high compared to β-TCP added blend composites, reinforcing the fact that scaffolds with good mechanical strength and enhanced porosity supports better cell adhesion.

Electrospun Biomimetic Nanofibrous Scaffolds: A Promising Prospect for Bone Tissue Engineering and Regenerative Medicine

“…SEM, EDAX, XRD, DSC, and FTIR were used to characterize the synthesized scaffolds’ physical, chemical, and thermal characteristics. MG-63 cell lines were used to test and compare the biocompatibility of the produced scaffolds [ 154 ]. The core-shell-type nanofibers were created by the electrospinning a cellulose acetate–PVP composite (a core made of cellulose acetate and a shell of PVP).…”

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“…(Figure 1(a)) showed the XRD of GOPVPA indicating the characteristic GO peak (101). Characteristic FTIR spectra of the composite reported in (Figure 1b) showed small absorption band around 1539 cm -1 which could be assigned to the characteristic vibration of C=N (pyridine ring) [14]. absorption band at 962 cm-1 is assigned to the out-of-plane rings C-H bending.…”

Synthesis and Characterization of CuO Nanoparticles and CuO/PVA-PVP Nanocomposites: Adsorption Activity for Malachite Green (MG) Dye and Antibacterial Property in Wastewater Treatment

“…The band 2923.94 cm -1 and 3221.69 cm -1 belong to symmetric and asymmetric stretching vibration of O-H bond, respectively [33], whereas for CuO/PVA-PVP NCs in Fig. 2(b) the peak at 586.23 cm -1 and 837.85 cm -1 indicated Cu-O stretching vibration [31] and C-C stretching vibration 1090.17 cm -1 is responsible for stretching C = O and bending of -OH, 1245.67 cm -1 is for C-N stretching vibration [34]. 1368.65 cm -1 , 1425.90 cm -1 appeared due to -CH2 bending and C-N stretching.…”