Preliminary Risk Assessment of a Novel Antifungal Defensin Peptide from Chickpea (Cicer arietinum L.)

Islam, Aparna

doi:10.1177/153567600801300405

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…Growth inhibition by plant defensins of a wide range of pathogenic fungi is not associated with toxicity in mammalian or plant cells (Thomma et al, 2002). Studies of the biological activity, stability, and range of toxicity of an isolated chickpea defensin (Ca-AFP) revealed that there are no risks in using the gene in the production of transgenic crops (Islam, 2008). However, to date, three defensin-related proteins have been described as allergens (Singh et al, 2006;Petersen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Field Resistance to Phakopsora pachyrhizi and Colletotrichum truncatum of Transgenic Soybean Expressing the NmDef02 Plant Defensin Gene

et al. 2020

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Fungal diseases lead to significant losses in soybean yields and a decline in seed quality; such is the case of the Asian soybean rust and anthracnose caused by Phakopsora pachyrhizi and Colletotrichum truncatum, respectively. Currently, the development of transgenic plants carrying antifungal defensins offers an alternative for plant protection against pathogens. This paper shows the production of transgenic soybean plants expressing the NmDef02 defensin gene using the biolistic delivery system, in an attempt to improve resistance against diseases and reduce the need for chemicals. Transgenic lines were assessed in field conditions under the natural infections of P. pachyrhizi and C. truncatum. The constitutive expression of the NmDef02 gene in transgenic soybean plants was shown to enhance resistance against these important plant pathogens. The quantification of the P. pachyrhizi biomass in infected soybean leaves revealed significant differences between transgenic lines and the non-transgenic control. In certain transgenic lines there was a strong reduction of fungal biomass, revealing a less severe disease. Integration and expression of the transgenes were confirmed by PCR, Southern blot, and qRT-PCR, where the Def1 line showed a higher relative expression of defensin. It was also found that the expression of the NmDef02 defensin gene in plants of the Def1 line did not have a negative effect on the nodulation induced by Bradyrhizobium japonicum. These results indicate that transgenic soybean plants expressing the NmDef02 defensin gene have a substantially enhanced resistance to economically important diseases, providing a sound environmental approach for decreasing yield losses and lowering the burden of chemicals in agriculture.

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Section: Introductionmentioning

confidence: 99%

Field Resistance to Phakopsora pachyrhizi and Colletotrichum truncatum of Transgenic Soybean Expressing the NmDef02 Plant Defensin Gene

et al. 2020

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“…3 Fungicide application is the most widely practiced method to control yield loss due to fungal attack. 4 Development of resistance in pathogens against conventional antifungal agents as well as growing concerns regarding the hazardous impact of fungicide on the environment necessitates the development of novel methods to control fungal pathogens. Genetic engineering has proved to be providing a good solution to this issue by transgenic expression of antifungal genes that confer resistance to fungal pathogens and enhance crop yield.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Plants are exposed to various biotic and abiotic stress factors such as drought and temperature, as well as attack by various pathogens and pests, which results in huge loss of crop yield worldwide amounting to 30–50 billion USD annually. , Fungal pathogens causes 70% of the major crop diseases . Fungicide application is the most widely practiced method to control yield loss due to fungal attack . Development of resistance in pathogens against conventional antifungal agents as well as growing concerns regarding the hazardous impact of fungicide on the environment necessitates the development of novel methods to control fungal pathogens.…”

Section: Introductionmentioning

confidence: 99%

Biological Safety Assessment of Mutant Variant of Allium sativum Leaf Agglutinin (mASAL), a Novel Antifungal Protein for Future Transgenic Application

Ghosh

Roy

Chakraborty

et al. 2013

J. Agric. Food Chem.

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Genetic engineering has established itself to be an important tool for crop improvement. Despite the success, there is always a risk of food allergy induced by alien gene products. The present study assessed the biosafety of mutant Allium sativum leaf agglutinin (mASAL), a potent antifungal protein generated by site directed mutagenesis of Allium sativum leaf agglutinin (ASAL). mASAL was cloned in pET28a+ and expressed in E. coli, and the safety assessment was carried out according to the FAO/WHO guideline (2001). Bioinformatics analysis, pepsin digestion, and thermal stability assay showed the protein to be nonallergenic. Targeted sera screening revealed no significant IgE affinity of mASAL. Furthermore, mASAL sensitized Balb/c mice showed normal histopathology of lung and gut tissue. All results indicated the least possibility of mASAL being an allergen. Thus, mASAL appears to be a promising antifungal candidate protein suitable for agronomical biotechnology.

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Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil-Borne Chickpea Fungal Pathogens

Nitnavare

Pothana

Yeshvekar

et al. 2022

J Plant Growth Regul

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Preliminary Risk Assessment of a Novel Antifungal Defensin Peptide from Chickpea (Cicer arietinum L.)

Abstract: Risk assessment of an antifungal peptide is a prerequisite before using the corresponding gene to produce broad-spectrum, fungal-resistant GM crops

Cited by 6 publications

References 37 publications

Field Resistance to Phakopsora pachyrhizi and Colletotrichum truncatum of Transgenic Soybean Expressing the NmDef02 Plant Defensin Gene

Field Resistance to Phakopsora pachyrhizi and Colletotrichum truncatum of Transgenic Soybean Expressing the NmDef02 Plant Defensin Gene

Biological Safety Assessment of Mutant Variant of Allium sativum Leaf Agglutinin (mASAL), a Novel Antifungal Protein for Future Transgenic Application

Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil-Borne Chickpea Fungal Pathogens

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