Preliminary Results to Establish the Daao System as an Alternative Selection Strategy on Apple

Hättasch, C.; Hanke, Magda‐Viola; Flachowsky, Henryk

doi:10.17660/actahortic.2009.814.39

“…(phosphomannose isomerase), GALT (UDP-glucose:galactose-1-phosphate uridyltransferase) (Joersbo et al 2003), atlD (arabitol dehydrogenase) (LaFayette et al 2005), dao1 (d-amino acid oxidase) (Erikson et al 2004;Hattasch et al 2009), ilvA (threonine deaminase) (Ebmeier et al 2004), dsdA (d-serine ammonia lyase) (Erikson et al 2005;Lai et al 2011), lyr (lysine racemase) (Chen et al 2010), D-LDH (D-lactate dehydrogenase) (Wienstroer et al 2012) and MSRB (Methionine sulfoxide reductase B) ). Unfortunately, these candidates have some defects.…”

Section: Pmimentioning

confidence: 99%

A novel non-antibiotic selectable marker GASA6 for plant transformation

Luo

¹

,

Gu

²

,

Wang

³

et al. 2021

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Selectable markers help the transformed cell/tissue to survive in an otherwise lethal exposure of an antibiotic or herbicide. Unfortunately, almost all the traditional selectable markers are antibiotic and herbicide resistance genes, which are controversial on human health concerns and environmental impact. Novel plant-derived, non-antibiotic, and non-herbicide selectable markers are urgently needed in plant transformation. Our previous work showed that the seedlings of overexpression Arabidopsis lines of AtGASA6 survived on medium with a high concentration of sugar, which leads to the hypothesis that AtGASA6 could be a selectable marker on media with high or low sugar content. In this study, leaf explants of AtGASA6 overexpression tobacco lines regenerated shoots on sugar-free shooting medium while those of wild type could not. Moreover, the seeds of AtGASA6 overexpression tobacco lines germinated and grew into normal seedlings on sugar-free MS medium while those of WT could not. Attractively, no developmental defects were observed in AtGASA6 transgenic progenies. Using AtGASA6 as a selectable marker, overexpression tobacco lines of GAI, which restrains plant size, were created on sugar-free media. The GAI overexpression lines had a smaller plant size than that of control. Considering its plant-derived and non-antibiotic nature, GASA6 is promising to be used as a selectable marker in plant transformation.

show abstract

“…2005), dao1 (d-amino acid oxidase) (Erikson et al 2004;Hattasch et al 2009), ilvA (threonine deaminase) (Ebmeier et al 2004), dsdA (d-serine ammonia lyase) (Erikson et al 2005;Lai et al 2011), lyr (lysine racemase) (Chen et al 2010), D-LDH (D-lactate dehydrogenase) (Wienstroer et al 2012) and MSRB (Methionine sulfoxide reductase B) (Li et (which was not certified by peer review) is the author/funder. All rights reserved.…”

Section: Pmimentioning

confidence: 99%

A novel non-antibiotic selectable marker GASA6 for plant transformation

Luo

¹

,

Gu

²

,

Wang

³

et al. 2021

Preprint

0

View full text Add to dashboard Cite

Selectable markers help the transformed cell/tissue to survive in an otherwise lethal exposure of an antibiotic or herbicide. Unfortunately, almost all the traditional selectable markers are antibiotic and herbicide resistance genes, which are controversial on human health concerns and environmental impact. Novel plant-derived, non-antibiotic, and non-herbicide selectable markers are urgently needed in plant transformation. Our previous work showed that the seedlings of overexpression Arabidopsis lines of AtGASA6 survived on medium with a high concentration of sugar, which leads to the hypothesis that AtGASA6 could be a selectable marker on media with high or low sugar content. In this study, leaf explants of AtGASA6 overexpression tobacco lines regenerated shoots on sugar-free shooting medium while those of wild type could not. Moreover, the seeds of AtGASA6 overexpression tobacco lines germinated and grew into normal seedlings on sugar-free MS medium while those of WT could not. Attractively, no developmental defects were observed in AtGASA6 transgenic progenies. Using AtGASA6 as a selectable marker, overexpression tobacco lines of GAI, which restrains plant size, were created on sugar-free media. The GAI overexpression lines had a smaller plant size than that of control. Considering its plant-derived and non-antibiotic nature, GASA6 is promising to be used as a selectable marker in plant transformation.

show abstract

“…2005), dao1 (d-amino acid oxidase) (Erikson et al 2004;Hattasch et al 2009), ilvA (threonine deaminase) (Ebmeier et al 2004), dsdA (d-serine ammonia lyase) (Erikson et al 2005;Lai et al 2011), lyr (lysine racemase) (Chen et al 2010), D-LDH (D-lactate dehydrogenase) (Wienstroer et al 2012) and MSRB (Methionine sulfoxide reductase B) ). Unfortunately, these candidates have some defects.…”

Section: Pmimentioning

confidence: 99%

A Novel Non-antibiotic Selectable Marker GASA6 for Plant Transformation

Luo

¹

,

Gu

²

,

Wang

³

et al. 2021

Preprint

0

View full text Add to dashboard Cite

Selectable markers help the transformed cell/tissue to survive in an otherwise lethal exposure of an antibiotic or herbicide. Unfortunately, almost all the traditional selectable markers are antibiotic and herbicide resistance genes, which are controversial on human health concerns and environmental impact. Novel plant-derived, non-antibiotic, and non-herbicide selectable markers are urgently needed in plant transformation. Our previous work showed that the seedlings of overexpression Arabidopsis lines of AtGASA6 survived on medium with a high concentration of sugar, which leads to the hypothesis that AtGASA6 could be a selectable marker on media with high or low sugar content. In this study, leaf explants of AtGASA6 overexpression tobacco lines regenerated shoots on sugar-free shooting medium while those of wild type could not. Moreover, the seeds of AtGASA6 overexpression tobacco lines germinated and grew into normal seedlings on sugar-free MS medium while those of WT could not. Attractively, no developmental defects were observed in AtGASA6 transgenic progenies. Using AtGASA6 as a selectable marker, overexpression tobacco lines of GAI , which restrains plant size, were created on sugar-free media. The GAI overexpression lines had a smaller plant size than that of control. Considering its plant-derived and non-antibiotic nature, GASA6 is promising to be used as a selectable marker in plant transformation.

show abstract

Preliminary Results to Establish the Daao System as an Alternative Selection Strategy on Apple

Cited by 7 publications

References 0 publications

A novel non-antibiotic selectable marker GASA6 for plant transformation

A novel non-antibiotic selectable marker GASA6 for plant transformation

A novel non-antibiotic selectable marker GASA6 for plant transformation

A Novel Non-antibiotic Selectable Marker GASA6 for Plant Transformation

Contact Info

Product

Resources

About