The aim of this work is the characterization of a Shack-Hartmann wavefront sensor prototype through a statistical analysis in accordance with the official Mexican standard. To determine the repeatability and reproducibility of ophthalmic aberration measurements, given in Zernike polynomials, a measurement protocol was proposed. The measurements were obtained using an experimental optical system, which uses a super luminescent diode (SLD) IR, as well as ophthalmic trial lenses to introduce optical aberrations, which are used as reference materials. The complete optical system is intended to be used as an experimental aberrometer to obtain low order aberrations of the human eye in vivo.