Preliminary Phytochemical Studies, GC-MS Analysis and In vitro Antioxidant Activity of Selected Medicinal Plants and its Polyherbal Formulation

Abstract

Background: Novel polyherbal formulation (PHF) is the utilization of more than one herb in the preparation of herbal medication. The thought is found in the conventional system of medicine where the variety of herbs in a specific proportion of illness. Because of synergism, polyherbalism presents a few advantages which aren't accessible in single herbal medication. It is utilized in these medications for the treatment of numerous sicknesses including antioxidants. Objective: To develop a phytochemical screening and GC-MS analysis of Novel Polyherbal formulation for In vitro antioxidant activity. Materials and Methods: Macroscopical, preliminary phytochemical, quantitative phytoconstituents, and In-vitro antioxidant activity of all the individual extract and polyherbal formulation was done by chemical method. Identification of phytoconstituents with the aid of Gas chromatography -Mass spectroscopy (GC-MS). Results: Macroscopical study and physicochemical examination, for example, ash value, extractive value, loss on drying, and pH were reported to A. racemosus, B. variegata, C. bonducella, S. asoka, and S. racemosus and novel polyherbal formulation. Qualitative phytochemical investigation revealed the presence of alkaloids, flavonoids, gums & mucilage, carbohydrates, steroids, proteins & amino acids, fats & fixed oils, glycoside, phenols, and saponins. Quantitative estimation such as TAC, TFC, TGC, TSC, and TPC was showed positive results. All the individual extract and PHF were subjected to GC-MS analysis. All the individual extract and polyherbal formulation displayed strong antioxidant activity. Conclusions: To conclude the PHF was reported that high level of bioactive contents present and strong antioxidant activity in contrast to the preferred ascorbic acid. The GC-MS uncovered the presence of bioactive compounds and these compounds are suggested to treat antibacterial, antioxidant, anti-inflammatory, and antiviral, anti-tumor, anti-proliferative activity, and antifungal activity.

Antioxidant properties and interaction effects of a novel polyherbal formulation

“…For example, the TPC of a polyherbal formulation prepared from various proportions of Centella asiatica (L.) Urb., Piper sarmentosum Roxb., and Morinda citrifolia L. ranged from 107.15-167.15 g GAE/ml, with individual TPC of 134.33, 125.7, and 209.12 g GAE/ml, respectively (16). Our result is also similar to the study reported that the TPC of a polyherbal formulation was within the range of those of its components (17).…”

Elicitation Induced α-Amyrin Synthesis in Tylophora indica In Vitro Cultures and Comparative Phytochemical Analyses of In Vivo and Micropropagated Plants

“…Phytochemical characterization is an immensely valuable step in detecting and quantifying novel bioactive compounds from in vitro-derived tissues (callus, somatic embryo and other plant parts). The application of GC–MS-based chemical profiling for the recognition and documentation of a diverse array of phytochemicals has been exemplified in many scientific reports [ 32 , 33 , 34 ]. In the present study, the GC–MS-based comparative analysis of the metabolites of tissue culture and field-grown (mother) plants was conducted in order to obtain the phytochemical profiles of two alternative sources of T. indica plants.…”

Effect of aqueous extract of barley and wheat grass in stress induced depression in Swiss mice

“…The anti-oxidant assay was done by using the methods explained in previous studies. DPPH ● scavenging activity [ 30 ], Hydrogen peroxide scavenging activity [ 31 ], Hydroxyl radical scavenging activity [ 32 ], Metal chelating activity [ 33 ], Nitric oxide scavenging activity [ 34 ], Ferric reducing power [ 34 ], and Total antioxidant activity [ 26 ].…”