Preliminary phytochemical and antimicrobial activity screening of crude extracts of bird lime (&lt;i&gt;Tapinanthus globiferus&lt;/i&gt;)

Emaikwu, V.; Ndukwe, IG; Iyun, Ogunkemi Risikat Agbeke; Anyam, J.Y.

doi:10.4314/jasem.v23i2.16

Cited by 7 publications

(7 citation statements)

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“…A total of 14 studies about the in vitro antibacterial activity of medicinal plants from Sub-Saharan Africa against Campylobacter spp. were found [23][24][25][26][27][28][29][30][31][32][33][34][35][36]. e majority of studies were from Nigeria (5 studies), South Africa (4), and Guinea-Bissau (3).…”

Section: Database Search Resultsmentioning

confidence: 99%

“…Nine studies tested the antimicrobial activity for Campylobacter spp. through the MIC [23,24,26,[29][30][31][33][34][35], while five studies tested only through disk diffusion method [25,27,28,32,36]. Table 1 provides a summary of the studied plant extracts, plant parts, isolated compounds, diameter of inhibition, MIC, and MBC of the tested plants.…”

Section: Results Of the In Vitromentioning

confidence: 99%

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A Systematic Review of In Vitro Activity of Medicinal Plants from Sub‐Saharan Africa against Campylobacter spp.

Hlashwayo

Barbosa

Langa

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Introduction. Campylobacter spp. are zoonotic bacteria that cause gastroenteritis in humans and may cause extraintestinal infections such as Guillain-Barré syndrome, reactive arthritis, and bacteremia. Resistance to antibiotics is an increasing concern in the Sub-Saharan Africa; thus, search for alternatives such as plant-based active ingredients is important in order to develop new drugs. Objectives. To present a systematic review of in vitro and in vivo studies of the antibacterial activity of medicinal plants from Sub-Saharan Africa against Campylobacter spp. Methodology. Studies published until March 2020 on medicinal plants used against Campylobacter spp. from each country of Sub-Saharan Africa were searched on PubMed, Science Direct, AJOL, and Google Scholar. Articles were selected based on the existence of information regarding in vitro and in vivo activity of medicinal plants against Campylobacter spp. Results. A total of 47 medicinal plants belonging to 28 families were studied for in vitro activity against Campylobacter spp. No plant was studied in vivo. Plants from Fabaceae family were the most commonly studied. The plants with the strongest antimicrobial activities were Cryptolepis sanguinolenta and Terminalia macroptera. The root extracts from these plants were effective, and both had a minimal inhibitory concentration (MIC) of 25 μg/ml. Seven pure compounds were isolated and analyzed for activity against Campylobacter spp. The compound cryptolepine from C. sanguinolenta was the most effective with MIC values ranging between 6.25 and 25 μg/ml. Conclusion. Several native plants from the Sub-Saharan Africa region were studied for in vitro activity against Campylobacter spp. Some plants seemed very effective against the bacteria. Chemical compounds from three plants have been isolated and analyzed, but further studies are needed in order to produce new and effective drugs.

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Section: Database Search Resultsmentioning

confidence: 99%

Section: Results Of the In Vitromentioning

confidence: 99%

A Systematic Review of In Vitro Activity of Medicinal Plants from Sub‐Saharan Africa against Campylobacter spp.

Hlashwayo

Barbosa

Langa

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Preliminary phytochemical screening of methanol leaf extract and it fractions(n-Hexane, ethylacetate, and n-butanol) of T. globiferus growing on Tamarindus indica leaves revealed the presence of saponins, tannins, alkaloids, cardiac glycoside, steroid, triterpenoids, phenols and flavonoids, ethylacetate and n-butanol fractions indicated the presence of similar constituents including flavonoids, alkaloids, tannins, saponins, phenols, steroid and cardiac glycoside while n-hexane contained only steroid, and triterpenoids as preseted in (Table .2).The presence of these phytochemical compounds in T. globiferus growing on other plants have been reported (Emaikwu et al, 2019). These secondary metabolites are thought to be responsible for the pharmacological activities of plant extract (Emaikwu et al, 2019).…”

Section: Phytochemical Screening Of Methanol Leaf Extract and It Frac...mentioning

confidence: 86%

Phytochemical and Antifungal Studies of Tapinathus globiferus Extract and Fractions

Tukur¹,

Abubakar²,

Yusuf³

2022

CaJoST

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T. globiferus growing on Tamarindus indica is a hemi-parasitic plant used in ethomomedicine to treat different ailment including fungal infections. The aim of the study was to conduct phytochemical and antifungal studies of T. globiferus growing on Tamarindus indica. The plant material was collected, identified and air dried. Powdered plant material was subjected to maceration using methanol to obtained crude methanol leaf extract which was partitioned using n-hexane, chloroform ethyl acetate and n- The extract and the fractions obtained were subjected to phytochemical evaluation sing standard protocols. The antifungal studies of the methanol extract and its fractions against Aspergillus fumugatus, Aspergillus niger, Trychophytom rubrum and Trychophyton mentagrophyte were investigated using agar well diffusion method at different concentration (100 - 1.25 mg/mL). The phytochemical screening revealed the presence of various secondary metabolites which varies in the extract and fractions. The methanol extract and its fractions showed significant (p< 0.05) antifungal activity against all the fungal isolates with the ethyl acetate fraction having the highest zone of inhibition ranging from 10-39 mm then followed by the n-butanol fraction with the mean zone of inhibitions from 10-28 mm. The methanol extract and the n-hexane fraction recorded lower zone of inhibition from 9-24 mm and 8-26 mm respectively. The standard drug ketoconazole had zone of inhibition from 6-22 mm. The most sensitive organism was A. niger while the least sensitive organisms were T. mentagraphyte and A. rubrum for the ethyl acetate fraction. The study has validated the ethnomedicinal claim for the use of this plant in treatment of the fungal infections. Keywords: Phytochemical; Tapinanthus globiferus; Tamarindus indica

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“…Previously, aqueous crude stem bark extract of a related species, Tapinanthus dodoneifolius (DC) Denser has exhibited anxiolytic and antidepressant effects in mice [33] and crude methanol leaf extract of T. globiferus has been shown to exert an antidepressant effect in mice [34], and aqueous residue fractions of T. globiferus have produced significant anticonvulsant effects in rodents [35,36]. Also, hydroalcoholic and aqueous leaf extracts and fractions of T. globiferus under investigation have demonstrated significant anxiolytic activity in rodent in-vivo protocols [37,38,39].…”

Section: Introductionmentioning

confidence: 99%

Anxiolytic Mechanism(S) and Corticosterone-Attenuating Effect of Hydroalcoholic Leaf Extract of Tapinanthus globiferus Mistletoe Growing on Azadirachta indica Tree

Umarudeen

Magaji

2020

JOCAMR

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Similar pharmacodynamic mechanism(s) often underlie drug actions and toxicities of anxiolytic agents and medicinal extracts. Extracts of Tapinanthus globiferus and related plant species have been reported with anxiolytic activities. But mechanistic evaluations on these plant extracts are few. This study investigated the anxiolytic mechanism(s), including the corticosterone-attenuating effect, of hydroalcoholic Tapinanthus globiferus (HATG) leaf extract harvested from Azadirachta indica host tree in the mouse elevated zero-maze and restraint-induced acute stress paradigms using per cent open segment time (%OST) and brain/plasma corticosterone levels as endpoints, respectively. The results show that anxiolytic activity (%OST) of 150 mg/kg HATG leaf extract was reversed by pretreatment with 5 mg/kg caffeine (HATG alone, 10.90±1.73;HATG+Caffeine, 8.66±1.74), 2 mg/kg methysergide (MTD) (HATG alone, 98.70±14.98; HATG+MTD, 74.20±10.82) and 5 mg yohimbine (HATG alone, 120.10±10.72; HATG+Yohimine, 78.44±13.92) but not by 0.5 m/kg atropine (HATG alone, 104.60±25.31; HATG+Atropine, 105.40±11.85), 0.5 mg/kg flumazenil (HATG alone,80.27±9.69; HATG+Flumazenil, 80.75±10.19), 2 mg/kg cyproheptadine (HATG alone, 88.67±16.44; HATG+Cyproheptadine, 92.11±12.58), 0.2 mg/kg haloperidol (HATG alone, 74.11±17.33; HATG+Haloperidol, 94.00±32.54) and 5 mg/kg naloxone (HATG alone, 94.30±10.84; HATG+Naloxone, 95.30±6.86). The results also indicate HATG leaf extract (at 50, 150, 500 and 1500 mg/kg) caused largely dose-dependent and significant (p<0.05) attenuations in brain/plasma corticosterone levels (5.64±0.66/3.91±0.44,3.78±0.39/3.39±0.38, 4.26±0.34/3.22±0.18 and 2.74±0.51/2.74±0.22), respectively, in extract- compared to distilled water- (5.93±0.60/4.56±0.37) and diazepam-treated (2.34±0.19/2.44±0.29) mice subjected to restraint-induced acute stress. These findings suggest anxiolytic mechanism(s) of the extract may involve its interactions with the adenosine, non-5HT2 serotonin, alpha (α)2 receptors and the hypothalamus-pituitary-adrenal (HPA) axis. This study may constitute the first mechanistic and corticosterone modulation report on the extracts of this parasitic medicinal plant and may benefit from confirmatory radio-labelled binding assays in subsequent studies.

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Preliminary phytochemical and antimicrobial activity screening of crude extracts of bird lime (<i>Tapinanthus globiferus</i>)

Cited by 7 publications

References 1 publication

A Systematic Review of In Vitro Activity of Medicinal Plants from Sub‐Saharan Africa against Campylobacter spp.

A Systematic Review of In Vitro Activity of Medicinal Plants from Sub‐Saharan Africa against Campylobacter spp.

Phytochemical and Antifungal Studies of Tapinathus globiferus Extract and Fractions

Anxiolytic Mechanism(S) and Corticosterone-Attenuating Effect of Hydroalcoholic Leaf Extract of Tapinanthus globiferus Mistletoe Growing on Azadirachta indica Tree

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