Preliminary Phytochemical Analysis and Invitro Antiviral Activity of Ethanolic extract of Whole plant of Tinospora cordifolia (Thunb.) Miers against Hepatitis-A Virus

Abstract

Herbal product design refers to the process of developing, standardizing, processing, and validating an herbal product for the market. Hepatitis A is an infectious disease of the liver caused by the hepatitis A virus (HAV). There are no specific medicines to cure infection with hepatitis A. Hence, there is a need for the development of less toxic, more efficacious and cost-effective antiviral agents. The present work highlighted the invitro anti-viral activity of ethanolic extract of whole plant of Tinospora cordifolia (Thunb.) Miers against HAV. Ethanolic extract of the selected plant was prepared by soxhlet apparatus and rotavap. For the antiviral screening against HAV, toxicological effect of selected plant extract was determined through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell proliferation assay using Huh-7 cell lines. The standard used is Camptothecin (CPT) at 25µG concentration. Plant extract was tested at 3.125 μg/mL, 6.25μg/mL,12.5μg/mL, 25 μg/mL and 50 μg/mL concentrations. The cell morphology was observed and recorded under microscope. The results clearly indicated the selected plant extract shows a dose dependent activity and it was maximum at 50 μg/mL concentration where it shows only 56.24% of virus viability and it was comparable to standard drug where it shows 43.01% of viability. Phytochemical screening of selected plant extract reveals the presence of different secondary metabolites like alkaloids, flavonoides, tannins, resins, steroids. Presence of these compounds in alone or combination might be responsible for the observed antiviral activity.

In silico studies reveal potential antiviral activity of phytochemicals from medicinal plants for the treatment of COVID-19 infection

“…India is a country rich with diverse repository of medicinal plants used for treatment of several human disorders. Tinospora cordifoliaalso known as amrita, guduchi, shindilkodi, giloy, is widely used in indigenous systems of Indian medicine [43].The chemical constitutents of T.cordifolia belong to different classes, namely terpenoids, alkanoids, glycosides, liganans, steroids among others [43][44][45].…”

Efficient extraction methods and callus culture of quercetin from Indian white radish: yield analysis via HPLC quantification and assessment of cytotoxic activity

“…3. These chemicals have been previously recognised in medicinal plant species, and quercetin is assumed to play a crucial role in plant defence mechanisms (Maddi et al ., 2018).…”

Yellow Medicine of Nature- The future Chartbuster Protoberberine Antivirals

“…For example, extracts prepared from the dried stem of Tinospora cordifolia, a protoberberine containing botanical exhibits useful properties in reducing HSV-1 infection (Purthvish and Gopinatha, 2018). Such plant-based medicines will be economical, more effective and less toxic (Maddi et al, 2018). However, extensive study of their antiviral property along with their mechanism has been only investigated regarding berberine.…”