Preliminary optimization of process conditions for biohydrogen production from sago wastewater

Ulhiza, Tami Astie; Puad, Noor Illi Mohamad; Azmi, Azlin Suhaida

doi:10.1109/icetss.2017.8324203

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…They reported that a 5% inoculum resulted in a higher hydrogen yield compared to a 9% inoculum size. The biological hydrogen yields were 52 µmol and 32 µmol for 5% and 9% inoculum sizes, respectively [37].…”

Section: Growth Behaviour Study and Biological Hydrogen Production Wi...mentioning

confidence: 97%

Biological Hydrogen Energy Production by Novel Strains Bacillus paramycoides and Cereibacter azotoformans through Dark and Photo Fermentation

et al. 2023

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In daily life, energy plays a critical role. Hydrogen energy is widely recognized as one of the cleanest energy carriers available today. However, hydrogen must be produced as it does not exist freely in nature. Various methods are available for hydrogen production, including electrolysis, thermochemical technology, and biological methods. This study explores the production of biological hydrogen through the degradation of organic substrates by anaerobic microorganisms. Bacillus paramycoides and Cereibacter azotoformans strains were selected as they have not yet been studied for biological hydrogen fermentation. This study investigates the ability of these microorganisms to produce biological hydrogen. Initially, the cells were identified using cell morphology study, gram staining procedure, and 16S ribosomal RNA (rRNA) gene polymerase chain reaction. The cells were revealed as Bacillus paramycoides (MCCC 1A04098) and Cereibacter azotoformans (JCM 9340). Moreover, the growth behaviour and biological hydrogen production of the dark and photo fermentative cells were studied. The inoculum concentrations experimented with were 1% and 10% inoculum size. This study found that Bacillus paramycoides and Cereibacter azotoformans are promising strains for hydrogen production, but further optimization processes should be performed to obtain the highest hydrogen yield.

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Section: Growth Behaviour Study and Biological Hydrogen Production Wi...mentioning

confidence: 97%

Biological Hydrogen Energy Production by Novel Strains Bacillus paramycoides and Cereibacter azotoformans through Dark and Photo Fermentation

et al. 2023

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“…Indirect biophotolysis, photofermentation, dark fermentation and hybrid system are the examples of biohydrogen process [2]. Dark fermentation is the production of biohydrogen from microorganisms using agricultural waste as the substrate [3]. E. aerogenes is a facultative anaerobe hydrogen producing bacteria that can alter the oxygen condition to anoxic condition that recovered the activity of Fe-hydrogenase to produce hydrogen via dark fermentation [4].…”

Section: Introductionmentioning

confidence: 99%

Acid Pretreatment of Sago Wastewater for Biohydrogen Production

2018

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Biohydrogen has been recognized to be one of the future renewable energy sources and has the potential in solving the greenhouse effects. In this study, Enterobacter aerogenes (E. aerogenes) was used as the biohydrogen producer via dark fermentation process using sago wastewater as the substrate. However, pretreatment of sago wastewater is required since it consists of complex sugars that cannot be utilized directly by the bacteria. This study aimed to use acid pretreatment method to produce high amount of glucose from sago wastewater. Three different types of acid: sulfuric acid (H2SO4); hydrochloric acid (HCl) and nitric acid (HNO3) were screened for the best acid in producing a maximum amount of glucose. H2SO4 gave the highest amount of glucose which was 9.406 g/L. Design of experiment was done using Face-centred Central Composite Design (FCCCD) tool under Response Surface Methodology (RSM) in Design Expert 9 software. The maximum glucose (9.138 g/L) was recorded using 1 M H2SO4 at 100 °C for 60 min. A batch dark fermentation using E. aerogenes was carried out and it was found that pretreated sago wastewater gave a higher hydrogen concentration (1700 ppm) compared to the raw wastewater (410 ppm).

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Preliminary optimization of process conditions for biohydrogen production from sago wastewater

Cited by 2 publications

References 18 publications

Biological Hydrogen Energy Production by Novel Strains Bacillus paramycoides and Cereibacter azotoformans through Dark and Photo Fermentation

Biological Hydrogen Energy Production by Novel Strains Bacillus paramycoides and Cereibacter azotoformans through Dark and Photo Fermentation

Acid Pretreatment of Sago Wastewater for Biohydrogen Production

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