Preliminary observations of a new approach to tissue repair: Peripheral blood mononuclear cells in platelet‐rich plasma injected into skin graft area

Abstract

Our purpose was accelerating the physiologic wound healing, stimulating tissue regeneration and the reparative tissue processes in resistant skin ulcers as in a case of an erosive lichen planus of the soles and after a surgical treatment as for severe Darier disease. The challenge was to establish an effective therapy to enhance tissue healing by the injection of a mixture of peripheral blood mononuclear cells (PB-MNCs) and platelet-rich plasma (PRP) into a skin autograft area. This new perioperative biotechnological approach enriches PRP with the effects of PB-MNCs. It offers a novel advanced strategy that could become an ideal biologic blood-derived therapy, whose components are entirely autologous and produced by a protocol independent by the operator.

Efficacy of intralesional injections of platelet‐rich plasma in patients with oral lichen planus: A pilot randomized clinical trial

“…In the present study, no significant statistical results were recorded between groups regarding remission score after 3 months of treatment, while El‐Komy et al ( 2015 ) reported longer remission when using intralesional PRP for treating oral erosions in PV patients. Orlandi et al ( 2018 ) reported long‐term remission in a case report of ulcerative skin lichen planus treated with autologous skin graft with intralesional PRP injections followed up for 4 years. As well as, long‐term remission have been reported after 2, 3 months of follow‐up for patients with EOLP treated with intralesional injections of PRP (Sethi Ahuja et al, 2020 ; Sobhy et al, 2020 ) respectively.…”

Thyroxine (T4) may promote re-epithelialisation and angiogenesis in wounded human skin ex vivo

“…Caution must be exercised with extrapolating the effects of T4 on human skin healing ex vivo to the more complex in vivo situation, as the model does not satisfactorily permit the study of the important contributions of platelets, neutrophils and circulating T cells to wound healing [72, 73]; while resident immunocytes like mast cells and macrophages are abundantly present and can be instructively studied ex vivo [74–77] Yet, the ex vivo effects of T4 reported here are well in line with its in vivo effects reported in rodent wound healing models [22, 31, 32]…”

Biologics in Microangiopathic Wounds

“…50 Peripheral blood mononuclear cells (PBMCs) cocultured with autologous platelet gel showed a modulation of the release of VEGF, bFGF, and IL10 and suggested a key role of platelets and their derived GFs, in the PBMCs-mediated wound healing process as in many other regenerative medical fields. 51 Orlandi et al 52 reported an interesting successful experience in erosive lichen planus of the soles (uncommon inflammatory ulcer) and ulceration after surgical treatment of severe cutaneous Darier disease, with the injection of PBMCs and PRP into skin autograft areas. The authors conclude that enrichment of PRP with the factors released by PBMCs may strongly enhance reparative skin processes.…”

“…Orlandi et al 52 reported an interesting successful experience in erosive lichen planus of the soles (uncommon inflammatory ulcer) and ulceration after surgical treatment of severe cutaneous Darier disease, with the injection of PBMCs and PRP into skin autograft areas. The authors conclude that enrichment of PRP with the factors released by PBMCs may strongly enhance reparative skin processes.…”