Preliminary isolated organ studies using an aqueous extract of Clivia miniata leaves

Veale, D.J.H.; Oliver, Douglas W.; Arangies, N.; Ki, Furman

doi:10.1016/0378-8741(89)90008-1

Cited by 29 publications

(11 citation statements)

References 1 publication

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“…For initial screening, an organ-based assay, utilising uterine tissue is therefore preferred. This technique is well established and has been used for testing plant extracts for uterine contraction (Veale et al, 1989(Veale et al, , 1999Kaido et al, 1997;Uguru et al, 1998;Cherian, 2000;Ghayur and Gilani, 2005;KamatenesiMugisha et al, 2007;Schou et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Uterine contraction induced by Tanzanian plants used to induce abortion

Nikolajsen

Nielsen

Rasch

et al. 2011

Journal of Ethnopharmacology

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Section: Introductionmentioning

confidence: 99%

Uterine contraction induced by Tanzanian plants used to induce abortion

Nikolajsen

Nielsen

Rasch

et al. 2011

Journal of Ethnopharmacology

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“…The transducer was previously calibrated to establish a relationship between the force applied to the transducer and the gauge deflection with a corresponding weight of 0.7 g. The tissue was mounted in a 50-ml organ bath containing continuously aerated De jalon's solution. [7] The temperature of the organ bath was maintained at 37°C by means of an external jacket through which warm water was circulated, while the tension on the tissue was maintained at 7 N. [8] The tissue was allowed to equilibrate for 45 minutes. [6] A pilot study was done to ascertain the effect of the ethanolic extract of A. cordifolia root on the uterus, and based on this study, doses of extract to be administered were selected (1, 10, and 50 mg/l).…”

Section: In Vitro Assay For Uterotonic Activitymentioning

confidence: 99%

Evaluation of the oxytocic activity of the ethanol extract of the roots of Alchornea cordifolia

Omonkhelin¹,

Buniyamin²,

Paul³

et al. 2010

Int J Green Pharm

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Alchornea cordifolia has been used traditionally for the induction of labour as an abortifacient. This study is aimed at verifying the folkloric use of the plant by investigating the effect of ethanolic extract of the root bark on the isolated stilboestrol pretreated uteri of non-pregnant female rats. The extract (1, 10, 50 g/l), oxytocin (4×10 −5 to 8×10 −3 g/l), acetylcholine (4×10 −6 to 8×10 −4 g/l), atropine (4×10 −3 g/l), phenoxybenzamine (4×10 −3 g/l), diphenhydramine(2×10 −1 g/l), and verapamil (12×10 −2 g/l) were used. Log concentration response curves were plotted and EC 50 and E max were obtained. One-way analysis of variance (ANOVA) with Dunnet corrections using Graph pad Instat version 2.05a was used for statistical analysis. The extract produced dose-dependent contraction of the uterus. Its potency was less than that of oxytocin and acetylcholine (P<0.05), but the E max showed no significant difference (P>0.05). The E max values of the extract in the presence of all antagonists were significantly reduced (P<0.01). The EC 50 in the presence of atropine showed no significant increase (P>0.05); however, in the presence of phenoxybenzamine, the increase was significant (P<0.05). The presence of diphenhydramine and verapamil produced an inhibition such that the EC 50 was unattainable. A. cordifolia stimulates the uterus possibly by binding to alpha-adrenergic or histaminergic receptors or both. This indicates the existence of active principles in the plant, which may be responsible for some of the applications in traditional medicines as an abortifacient and in the induction of labour.

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“…In a survey by 488 Abdillahi and Van Staden (2013) (Viladomat et al, 1997;Nair et al, in press;Abdillahi and Van Sta-495 (Veale et al, 1989). Furthermore, the 499 same extracts were shown to have a synergistic effect at low con-500 centrations of acetylcholine, but a reduced response was elicited at 501 high concentrations (Veale et al, 1989). In relation to active com-502 ponents, it was shown that linoleic acid and 5-hydroxymethyl-2-503 furanocarboxaldehyde were both capable of inducing uterotonic 504 smooth muscle contraction in vitro in guinea pigs (Sewram et al,505 2001).…”

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confidence: 94%

“…The 485 accessibility and affordability of TM coupled to the support affor-486 ded by extended family structures make these practices more con-487 venient to these societies (Veale et al, 1992). In a survey by 488 Abdillahi and Van Staden (2013) (Viladomat et al, 1997;Nair et al, in press;Abdillahi and Van Sta-495 (Veale et al, 1989). Furthermore, the 499 same extracts were shown to have a synergistic effect at low con-500 centrations of acetylcholine, but a reduced response was elicited at 501 high concentrations (Veale et al, 1989).…”

mentioning

confidence: 96%