Preliminary investigations on the standardization and quality control for the determination of acid phosphatase activity in seminal plasma

Lü, Jin-Chun; Chen, Fang; Xu, Hui-Ru; Huang, Yu‐Feng; Lü, Nian-Qing

doi:10.1016/j.cca.2006.06.005

Cited by 18 publications

(11 citation statements)

References 32 publications

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“…The result showed that there was a significant difference of γ ‐GT levels ( P < 0.001) and sperm concentrations ( P < 0.001) in seminal plasma obtained after centrifugation at 1000 g for 10 min and 3000 g for 15 min respectively. However, there was no significant difference of acid phosphatase levels in seminal plasma obtained at different velocity centrifugation (Lu et al. , 2007), indicating that centrifugation at 3000 g may not release more enzyme from spermatozoa than that at 1000 g .…”

Section: Discussionmentioning

confidence: 96%

Preliminary investigations on the standardisation and quality control for the determination of ?-glutamyltranspeptidase activity in seminal plasma

Chen

Lü

et al. 2007

Andrologia

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The present study was designed to assess the effects of centrifugation velocity, standing time after dilution, freezing-thawing and chymotrypsin on the determination of gamma-glutamyltranspeptidase (gamma-GT) activities in seminal plasma, and to establish an instruction for the standardisation and quality control for the determination of gamma-GT within the same laboratory and among different laboratories. The gamma-GT level and sperm concentration of each of 72 samples of seminal plasma obtained by centrifugation at 1000 g for 10 min or 3000 g for 15 min were assayed. In addition, gamma-GT activities in diluted seminal plasma with different standing time and in samples with or without chymotrypsin were measured. The results showed that there was a significant difference of gamma-GT levels in seminal plasma obtained by centrifugation at different velocities (P < 0.001), and that gamma-GT activities in seminal plasma measured after standing for 30 min after dilution were notably lower than those measured immediately after dilution (P < 0.001). However, the data indicated that both chymotrypsin and freezing-thawing had no apparent effect on the determination of seminal gamma-GT. In conclusion, standing time after dilution and centrifugation velocity should be standardised, and frozen seminal plasma could serve as quality control products for the determination of gamma-GT activity among different laboratories.

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Section: Discussionmentioning

confidence: 96%

Preliminary investigations on the standardisation and quality control for the determination of ?-glutamyltranspeptidase activity in seminal plasma

Chen

Lü

et al. 2007

Andrologia

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“…[7][8][9][10][11]23 Our results have shown that the standardization of the determination of the seminal biochemistry markers, such as fructose, alpha-glucosidase, and acid phosphatase, is very important. [8][9][10][11] In order to guarantee the accuracy of a seminal UA assay, we have attempted to establish seminal UA detection and investigate the standardization and quality control for the determination of seminal UA levels.…”

Section: Discussionmentioning

confidence: 99%

“…7,8 A series of investigations has been carried out on the standardization and quality control for seminal biochemistry markers, including alpha-glucosidase, fructose, acid phosphatase, and gamma-glutamyl transpeptidase. [8][9][10][11] Nevertheless, the standardization and quality control for the determination of uric acid (UA) levels in seminal plasma has been poorly documented.…”

Section: Sciencementioning

confidence: 99%

Standardization and Quality Control for the Determination of Uric Acid Level in Seminal Plasma

Zhang¹,

Lü²,

Huang³

et al. 2009

Lab Med

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Jinling Hospital) was dissolved with 0.5 mL of normal saline to make 10 mg/mL of stock solution. Both low ([18 ± 2.5]×10 6 /mL) and high ([35 ± 5]×10 6 /mL) precalibrated standard latex bead solutions (Hamilton Thorne Biosciences, USA) were used as the quality control solution for sperm concentration. Samples ResourceSemen samples were collected from outpatients in the Department of Andrology, Nanjing Jinling Hospital. After liquefaction, routine analysis for each of the semen samples was performed, including semen volume, pH, sperm concentration, motility, and grade a and b motility. The remaining portions of the samples were used in our investigation.

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“…At present, quality controls for determinations of sperm concentration, motility, morphology, and antisperm antibodies have been emphasized widely (Cooper et al, 2002; Toft et al, 2005). However, few reports exist on standardization and quality control for biochemical analysis of seminal plasma (Lu et al, 2007). Therefore, we attempted to establish standardization and quality control for the determination of fructose concentration in seminal plasma.…”

mentioning

confidence: 99%

Standardization and Quality Control for Determination of Fructose in Seminal Plasma

Lü

Chen

et al. 2007

Journal of Andrology

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Preliminary investigations on the standardization and quality control for the determination of acid phosphatase activity in seminal plasma

Cited by 18 publications

References 32 publications

Preliminary investigations on the standardisation and quality control for the determination of ?-glutamyltranspeptidase activity in seminal plasma

Preliminary investigations on the standardisation and quality control for the determination of ?-glutamyltranspeptidase activity in seminal plasma

Standardization and Quality Control for the Determination of Uric Acid Level in Seminal Plasma

Standardization and Quality Control for Determination of Fructose in Seminal Plasma

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