Preliminary investigation of Cu-containing proteins in seeds of Brazil nuts by ICPMS and MALDI-MS methods

Jayasinghe, Sarath B.; Caruso, Joseph A.

doi:10.1016/j.ijms.2013.09.014

Cited by 11 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…48 The total Hg content in the homogenised sh material was determined by IDMS, with the 202 Hg spike added prior to a hot nitric acid digestion procedure. Finally, the 200 Hg : 201 Hg isotope amount ratio was measured in the column eluent by a single collector SF-ICP-MS instrument. However, it can be surmised from the method used to assess sample homogeneity that the candidate CRM was subjected to a 6 mol L À1 HCl leach procedure, followed by retention of the Hg in the leachate on an anion exchange column (Dowex 1 Â 8 cm, 100-200 mesh), UV oxidation of the MeHg in the eluent to Hg II and measurement by FI-CV-AAS.…”

Section: Crms and Metrologymentioning

confidence: 99%

“…For the MeHg content the method is less clear as it is only partially reported. The mass bias correction factor was determined by the measurement of the 200 Hg : 201 Hg isotope amount ratio using the bracketing method. The procedure was validated by the analysis of two NRCC CRMs, namely DORM-2 and DOLT-2.…”

Section: Crms and Metrologymentioning

confidence: 99%

“…200 Hg 2+ , with 0.1% v/v 2-mercaptoethanol (2-ME), 0.1% v/v HCl and 0.05% m/v L-cysteine and UAE for 30 min. Aer sonication, the samples were centrifuged and the supernatant retained for analysis.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Atomic Spectrometry Update: review of advances in elemental speciation

Harrington

Clough

Hill

et al. 2015

J. Anal. At. Spectrom.

View full text Add to dashboard Cite

show abstract

Section: Crms and Metrologymentioning

confidence: 99%

Section: Crms and Metrologymentioning

confidence: 99%

See 1 more Smart Citation

Atomic Spectrometry Update: review of advances in elemental speciation

Harrington

Clough

Hill

et al. 2015

J. Anal. At. Spectrom.

View full text Add to dashboard Cite

show abstract

“…Many recent studies have explored the properties of Cu 2+ , considering that it has an antibacterial property [20][21][22]. Cu 2+ is an essential element for living organisms [22,23]. It is a vital trace element that plays a key role in immunity, increasing the average rate of bone resorption in bone metabolism and improving collagen fiber precipitation [20,24,25].…”

Section: Introductionmentioning

confidence: 99%

Influence of Cu2+ Ions on the Corrosion Resistance of AZ31 Magnesium Alloy with Microarc Oxidation

Ahmed

Zhang

et al. 2020

Materials

View full text Add to dashboard Cite

The objectives of this study were to reduce the corrosion rate and increase the cytocompatibility of AZ31 Mg alloy. Two coatings were considered. One coating contained MgO (MAO/AZ31). The other coating contained Cu2+ (Cu/MAO/AZ31), and it was produced on the AZ31 Mg alloy via microarc oxidation (MAO). Coating characterization was conducted using a set of methods, including scanning electron microscopy, energy-dispersive spectrometry, X-ray photoelectron spectroscopy, and X-ray diffraction. Corrosion properties were investigated through an electrochemical test, and a H2 evolution measurement. The AZ31 Mg alloy with the Cu2+-containing coating showed an improved and more stable corrosion resistance compared with the MgO-containing coating and AZ31 Mg alloy specimen. Cell morphology observation and cytotoxicity test via Cell Counting Kit-8 assay showed that the Cu2+-containing coating enhanced the proliferation of L-929 cells and did not induce a toxic effect, thus resulting in excellent cytocompatibility and biological activity. In summary, adding Cu ions to MAO coating improved the corrosion resistance and cytocompatibility of the coating.

show abstract

“…To this end, a variety of analytical methods have been used to analyse metalloproteomes including the bioinformatic approach, 3 radioactive metal detection methods, [4][5][6] colorimetric assays, 7,8 two-dimensional diagonal sodium dodecyl sulphate-polyacrylamide gel electrophoresis, 9 and a combination of ICP and MALDI-MS methods. 10,11 Immobilized metal ion affinity chromatography (IMAC) is one of the best strategies in protein pre-fractionation in which specific protein fractions can be efficiently isolated from protein mixtures by designing particular binding ligand metal interactions. 12 Hence, a large number of IMAC strategies have been developed in the case of pre-fractionate proteins for metalloproteomic investigation by immobilizing different metal ions, 13 such as Zn 2+ , Ni 2+ , Cu 2+ and Co 2+ .…”

Section: Introductionmentioning

confidence: 99%

Selective enrichment of metal-binding proteins based on magnetic core/shell microspheres functionalized with metal cations

Fang

Zhang

et al. 2015

Analyst

View full text Add to dashboard Cite

Metal binding proteins play many important roles in a broad range of biological processes. Characterization of metal binding proteins is important for understanding their structure and biological functions, thus leading to a clear understanding of metal associated diseases. The present study is the first to investigate the effectiveness of magnetic microspheres functionalized with metal cations (Ca(2+), Cu(2+), Zn(2+) and Fe(3+)) as the absorbent matrix in IMAC technology to enrich metal containing/binding proteins. The putative metal binding proteins in rat liver were then globally characterized by using this strategy which is very easy to handle and can capture a number of metal binding proteins effectively. In total, 185 putative metal binding proteins were identified from rat liver including some known less abundant and membrane-bound metal binding proteins such as Plcg1, Acsl5, etc. The identified proteins are involved in many important processes including binding, catalytic activity, translation elongation factor activity, electron carrier activity, and so on.

show abstract

Preliminary investigation of Cu-containing proteins in seeds of Brazil nuts by ICPMS and MALDI-MS methods

Cited by 11 publications

References 22 publications

Atomic Spectrometry Update: review of advances in elemental speciation

Atomic Spectrometry Update: review of advances in elemental speciation

Influence of Cu2+ Ions on the Corrosion Resistance of AZ31 Magnesium Alloy with Microarc Oxidation

Selective enrichment of metal-binding proteins based on magnetic core/shell microspheres functionalized with metal cations

Contact Info

Product

Resources

About