Preliminary investigation demonstrating the GHITM gene probably involved in apoptosis and growth of the golden apple snail (Pomacea canaliculata)

Yu, Wenchao; Zhang, Baolu; Song, Hongce; Zhan, Rui; He, Cheng; Jiang, Qiuyun; Wang, Xiaona; Wei, Lei; Zhao, Nannan; Guo, Wen; Wang, Xiaotong

doi:10.1186/s12864-019-6434-2

Cited by 6 publications

(8 citation statements)

References 40 publications

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“…TMBIM5 was previously linked to apoptotic pathways [ 15 , 61 ]. High TMBIM5 expression levels are considered a favorable prognostic marker for survival in renal cancer while being unfavorable in head and neck cancer [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

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Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery

Seitaj

Maull

Zhang

et al. 2020

Cells

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The Transmembrane Bax Inhibitor-1 motif (TMBIM)-containing protein family is evolutionarily conserved and has been implicated in cell death susceptibility. The only member with a mitochondrial localization is TMBIM5 (also known as GHITM or MICS1), which affects cristae organization and associates with the Parkinson’s disease-associated protein CHCHD2 in the inner mitochondrial membrane. We here used CRISPR-Cas9-mediated knockout HAP1 cells to shed further light on the function of TMBIM5 in physiology and cell death susceptibility. We found that compared to wild type, TMBIM5-knockout cells were smaller and had a slower proliferation rate. In these cells, mitochondria were more fragmented with a vacuolar cristae structure. In addition, the mitochondrial membrane potential was reduced and respiration was attenuated, leading to a reduced mitochondrial ATP generation. TMBIM5 did not associate with Mic10 and Mic60, which are proteins of the mitochondrial contact site and cristae organizing system (MICOS), nor did TMBIM5 knockout affect their expression levels. TMBIM5-knockout cells were more sensitive to apoptosis elicited by staurosporine and BH3 mimetic inhibitors of Bcl-2 and Bcl-XL. An unbiased proteomic comparison identified a dramatic downregulation of proteins involved in the mitochondrial protein synthesis machinery in TMBIM5-knockout cells. We conclude that TMBIM5 is important to maintain the mitochondrial structure and function possibly through the control of mitochondrial biogenesis.

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Section: Discussionmentioning

confidence: 99%

“…In animal models, TMBIM5 downregulation impaired growth processes in snails [ 61 ] and reduces lifespan in flies [ 64 ]. This is in line with our data that demonstrated reduced proliferation and a reduced size of TMBIM5-KO cells.…”

Section: Discussionmentioning

confidence: 99%

Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery

Seitaj

Maull

Zhang

et al. 2020

Cells

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“…29 Growth hormone-induced transmembrane protein (GHITM) is a highly conserved transmembrane protein. 30 The Transmembrane Bax interactivator -1 Motif (TMBIM) protein family is evolutionarily conserved and associated with susceptibility to cell death. The only member of this protein expressed in mitochondria is TMBIM5 (also known as GHITM or MICS1).…”

Section: Discussionmentioning

confidence: 99%

Identification of Genetic Markers and Immune Infiltration Characteristics of Alzheimer’s Disease Through Weighted Gene Co-expression Network Analysis

Qiang

Duan

Miao

et al. 2022

Preprint

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Background: Alzheimer disease (AD) is a long-term progressive neurodegeneration disease. Studies have shown that accumulation of epigenetic changes promotes the formation of gene mutations thereby inducing the pathogenesis of Alzheimer disease. Currently, the molecular mechanisms of alzheimer disease are not well defined. This calls for identification of genetic markers of alzheimer disease which can be leveraged to design effective drugs. Method: In this study, we aimed to determine the modules and hub genes that contribute to the development of alzheimer disease through weighted gene co-expression network analysis (WGCNA). The genes in the module were enriched and analyzed by GO / KEGG, and the protein-protein interaction（PPI） network was constructed. In addition, WGCNA topology analysis was carried out to construct a gene network, from which five hub genes were identified. Logistic regression analysis and Receiver operating characteristics (ROC) was performed to explore the clinical value of genes in diagnosis of Alzheimer. The genes in the core module are intersected with the hub genes, and four intersecting genes were selected, which were ATP2A2, ATP6V1D, CAP2 and SYNJ1. The four genes were enriched by GSEA. Finally, immune infiltration analysis was performed.Results: The GO/KEGG analysis shows that the genes in the core module play a role in the differentiation and growth of nerve cells and neurotransmitter transmission.Gene set enrichment analysis (GSEA) about core genes demonstrated that the four genes were mainly enriched in immune infection pathways such as cholera infection and Helicobacter pylori infection and other metabolic pathways. In addition, immune infiltration characteristics were investigated and found that T cells regulatory (Tregs), neutrophils, plasma cells, mast cells activated, T cells cellular helper, T cells CD8, T cells CD4 memory resetting and macrophases M1 are core immune cells contributing to the progression of Alzheimer's disease. Conclusion: The results of enrichment analysis and immunoosmotic analysis showed that immune pathways and immune cells played an important role in the occurrence and development of alzheimer disease. The selected key genes are used as biomarkers related to the pathogenesis of alzheimer disease to further explore the pathways and cells.

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“…As the other tree topologies shown in Figure 4d–f provide support for a clade that includes all ectoproct and bryozoan species, these genes may be associated with the differentiation of multiciliated cells. A combined functional synthesis gathered for all of the cellular and developmentally related genes listed here results in some commonalities among their respective roles such as immune response ( CNPY4 ; Takahashi et al, 2007), mRNA processing and translation ( EIF1 , EIF1AD , and TARDBP ; Buratti & Baralle, 2008; Mitchell & Lorsch, 2008; J. Yu & Marintchev, 2018), cell cycle progression, cell proliferation, and cell growth ( GADD45GIP1 , GHITM, IER5 , EIF1, EIF1AD , and USF2 ; Fujimi & Aruga, 2008; Kwon et al, 2009; Sehrawat et al, 2019; Xiong, Jiang, Liu, Zhou, & Ding, 2019; J. Yu & Marintchev, 2018; W. Yu et al, 2020), and the development of the nervous system and cilia ( CFAP126 , RSPH9 , USF2 , SEC13 , and VEGFA ; Erskine et al, 2017; Fujimi & Aruga, 2008; Gegg et al, 2014; Niu et al, 2014; Sedykh et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Yu & Marintchev, 2018), cell cycle progression, cell proliferation, and cell growth (GADD45GIP1, GHITM, IER5, EIF1, EIF1AD, and USF2; Fujimi & Aruga, 2008;Kwon et al, 2009;Sehrawat et al, 2019;Xiong, Jiang, Liu, Zhou, & Ding, 2019;J. Yu & Marintchev, 2018;W. Yu et al, 2020), and the development of the nervous system and cilia (CFAP126, RSPH9, USF2, SEC13, and VEGFA; Erskine et al, 2017;Fujimi & Aruga, 2008;Gegg et al, 2014;Niu et al, 2014;Sedykh et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Genes with evidence of positive selection as potentially related to coloniality and the evolution of morphological features among the lophophorates and entoprocts

Santagata

2020

J Exp Zool Pt B

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Evolutionary mechanisms that underlie the origins of coloniality among organisms are diverse. Some animal colonies may be comprised strictly of clonal individuals formed from asexual budding or comprised of a chimera of clonal and sexually produced individuals that fuse secondarily. This investigation focuses on select members of the lophophorates and entoprocts whose evolutionary relationships remain enigmatic even in the age of genomics. Using transcriptomic data sets, two coloniality‐based hypotheses are tested in a phylogenetic context to find candidate genes showing evidence of positive selection and potentially convergent molecular signatures among solitary species and taxa‐forming colonies from aggregate groups or clonal budding. Approximately 22% of the 387 orthogroups tested showed evidence of positive selection in at least one of the three branch‐site tests (CODEML, BUSTED, and aBSREL). Only 12 genes could be reliably associated with a developmental function related to traits linked with coloniality, neuroanatomy, or ciliary fields. Genes testing for both positive selection and convergent molecular characters include orthologues of Radial spoke head, Elongation translation initiation factors, SEC13, and Immediate early response gene5. Maximum likelihood analyses included here resulted in tree topologies typical of other phylogenetic investigations based on wider genomic information. Further genomic and experimental evidence will be needed to resolve whether a solitary ancestor with multiciliated cells that formed aggregate groups gave rise to colonial forms in bryozoans (and perhaps the entoprocts) or that the morphological differences exhibited by phoronids and brachiopods represent trait modifications from a colonial ancestor.

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Preliminary investigation demonstrating the GHITM gene probably involved in apoptosis and growth of the golden apple snail (Pomacea canaliculata)

Cited by 6 publications

References 40 publications

Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery

Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery

Identification of Genetic Markers and Immune Infiltration Characteristics of Alzheimer’s Disease Through Weighted Gene Co-expression Network Analysis

Genes with evidence of positive selection as potentially related to coloniality and the evolution of morphological features among the lophophorates and entoprocts

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