Preliminary insights into the non structural protein 3 macro domain of the Mayaro virus by powder diffraction

Abstract

Abstract. We present preliminary structural results of the non-structural protein 3 (nsP3) macro domain from the Mayaro virus (MAYV), an emerging virus of South American tropic regions, by means of synchrotron X-ray powder diffraction. Indexing of the diffraction patterns indicate a trigonal/hexagonal lattice (a ¼ 61:60 A, c ¼ 94:61 A), analogous to the known lattice of the sequence homologous nsP3 macro domain from the Chikungunia virus (CHIKV), though MAYV must have looser molecular packing: the cell dimensions of MAYV are significantly altered in comparison to CHIKV and the unit cell comprises 6 molecules and 58% solvent. The results are discussed in terms of their methodological and biological importance.

Revisiting the structure of a synthetic somatostatin analogue for peptide drug design

“…Significant methodological advances over the past decades transformed macromolecular XPD from an impossible task to a respectable method, circumventing the need for a suitable single crystal of the molecule of interest (Margiolaki & Wright, 2008;Karavassilia & Margiolaki, 2016). This approach has already been effectively employed for phase identification (Basso et al, 2005;Norrman et al, 2006;Collings et al, 2010;Papageorgiou et al, 2010;Karavassili et al, 2012;Valmas et al, 2015;Fili et al, 2015Fili et al, , 2016Trampari et al, 2018) as well as structure determination (Margiolaki et al, 2005(Margiolaki et al, , 2007(Margiolaki et al, , 2013Valmas et al, 2017) of a wide range of proteins and peptides (Tedesco et al, 2000(Tedesco et al, , 2001Cheung et al, 2002;Inouye & Kirschner, 2006;Fujii et al, 2011;Das et al, 2015) of pharmacological interest.…”

NMR study of non-structural proteins—part I: 1H, 13C, 15N backbone and side-chain resonance assignment of macro domain from Mayaro virus (MAYV)

“…Although a preliminary attempt has been reported (Papageorgiou et al 2010), there is no available high-resolution structural information for MAYV macro domain. Therefore, the present study provides significant insight into the conformational properties of this domain in solution and contributes to understanding its functions.…”

Applications of X-ray Powder Diffraction in Protein Crystallography and Drug Screening

“…The latter was originally confirmed a decade ago, when the macro domain moiety of the nsP3 protein from the Mayaro virus (MAYV), which appears in tropic regions of South America, was investigated. Despite significant efforts, good quality single crystals of the MAYV nsP3 macro domain were not available, whereas crystallization trials resulted in reproducible needle-shaped microcrystalline samples and the first structural information was obtained via synchrotron XRPD measurements [69]. X-ray diffraction data were collected at the European Synchrotron Radiation Facility (ESRF) while indexing of the diffraction patterns indicated a trigonal/ hexagonal unit cell (space group: P3 1 , a = 61.603 (3) Å, c = 94.619 (5) Å) ( Figure 2).…”

“…X-ray diffraction data were collected at the European Synchrotron Radiation Facility (ESRF) while indexing of the diffraction patterns indicated a trigonal/ hexagonal unit cell (space group: P3 1 , a = 61.603 (3) Å, c = 94.619 (5) Å) ( Figure 2). The high-resolution powder diffraction beamline ID31, RT, λ = 1.29984 Å. Insets correspond to magnifications of profile selected regions [69].…”

“…Upper Panel: The materials science beamline ID11, RT, λ = 0.3492 Å. Lower Panel: The high-resolution powder diffraction beamline ID31, RT, λ = 1.29984 Å. Insets correspond to magnifications of profile selected regions [69].…”