Preliminary identification and coat protein gene phylogenetic relationships of begomoviruses associated with native flora and cultivated plants from the Yucatan Peninsula of Mexico

Abstract

A number of native and cultivated eudicots in the Yucatan Peninsula of Mexico (YPM) exhibit symptoms associated with virus infection. Symptomatic leaves were collected and assessed for begomoviral detection using polymerase chain reaction (PCR), and universal primers that amplify a fragment of the coat protein gene (core Cp). Begomovirus were detected in nine native and seven cultivated species, representing seven eudicot families. DNA extracts from the 16 hosts were used for PCR amplification and sequencing of a fragment containing the coat protein (Cp) gene. The complete Cp sequence was used to establish provisional species identification. Results indicated that 13 distinct begomovirus species were represented. Among these, five potentially new begomovirus species were identified, for which we propose the names Anoda golden mosaic virus (AnGMV), Boerhavia yellow spot virus (BoYSV), Papaya golden mosaic virus (PaGMV), Desmodium leaf distortion virus (DeLDV), and Hibiscus variegation virus (HiVV). Five previously described begomoviral species were provisionally identified for the first time in the YPM; these include Euphorbia mosaic virus (EuMV), Melon chlorotic leaf curl virus (MCLCuV), Okra yellow mosaic Mexico virus (OkYMMV), Sida golden mosaic virus (SiGMV), and Tobacco apical stunt virus (TbASV). Additionally, viruses previously reported from this region, Bean golden yellow mosaic virus (BGYMV), Pepper golden mosaic virus (PepGMV), and Tomato mottle virus (ToMoV) were provisionally identified in cultivated hosts. Phylogenetic analysis provisionally placed all isolates from the YPM in a Western Hemisphere begomovirus clade.

Molecular characterization and phylogenetic relationships of Desmodium leaf distortion virus (DeLDV): a new begomovirus infecting Desmodium glabrum in Yucatan, Mexico

“…Begomoviruses have been reported in the Yucatan Peninsula of Mexico (YPM) from cultivated plants, including bean [2], pepper [3], and tomato [4]. Begomoviruses also have been identified in native species from a number of different plant families in the YPM [5].…”

“…In the YPM, this species is broadly distributed among different habitats. Symptomatic D. glabrum plants have been associated with an undescribed begomovirus, referred to as Desmodium leaf distortion virus (DeLDV), however, characterization was limited to partial sequence of the coat protein gene [5].…”

“…Native or endemic plant species have been implicated as natural hosts of begomoviruses in the YPM [5], making this an important biotic province for studying begomovirus diversity. According to criteria for begomovirus species demarcation, which is based on[89% shared nt identity [9], our results indicate that DeLDV is a new, previously undescribed bipartite begomovirus species.…”

“…Phylogenetic analyses were done as previously described [5]. The data set for DNA-A analysis consisted of 44 taxa.…”

Virus asociados al amarillamiento de Hibiscus sabdariffa en Guerrero, México

“…Los bajos porcentajes de similaridad obtenidos en el caso del CdTV, MAV y TbLCCV posiblemente se deban a que se trata de begomovirus no descritos hasta el momento. En México se ha reportado al Hibiscus variegado virus (HVV) en Hibiscus sabdariffa en la península de Yucatán (Hernández-Zepeda et al, 2007), mientras que en la India, Roy et al, (2009) …”