Preliminary Experiments on the Mechanism of Citrate Fermentation in Yeasts

Tabuchi, Takeshi; Tahara, Yasutaka; Tanaka, Masayuki; Yanagiuchi, Shihoko

doi:10.1271/nogeikagaku1924.47.617

Cited by 10 publications

(4 citation statements)

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“…Another fact being controvertible to the hypothesis was that thiamine deficiency in glucose-grown cells caused increases of the activities of NAD-and NADPlinked isocitrate dehydrogenases but did not change the activity of aconitate hydratase in the parent strain. 24) In the parent strain, the respective enzyme levels of the cells grown on the odd-numbered alkane were similar to those of the cells grown on the even-numbered alkane (Fig. 2).…”

Section: Effect Of Carbon Sources On Enzyme Synthesis Of the Mutant Ssupporting

confidence: 53%

Regulation of Enzyme Synthesis of the Glyoxylate, the Citric Acid, and the Methylcitric Acid Cycles inCandida lipolytica

Tabuchi

Igoshi²

1978

Agricultural and Biological Chemistry

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Section: Effect Of Carbon Sources On Enzyme Synthesis Of the Mutant Ssupporting

confidence: 53%

Regulation of Enzyme Synthesis of the Glyoxylate, the Citric Acid, and the Methylcitric Acid Cycles inCandida lipolytica

Tabuchi

Igoshi²

1978

Agricultural and Biological Chemistry

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“…The optimum pH for the yeast citrate process is above 5.0. Lower pH values lead to production of polyhydroxy compounds such as erythritol and arabitol (Tabuchi et al, 1973). The yeast process yields a conversion of 140-150% based on hydrocarbon used, a productivity of 1.4 g l -1 h -1 and a broth concentration as high as 225 g l -1 .…”

Section: Citric Acid Production Of Citric Acid By a Niger And Yeastmentioning

confidence: 99%

“…The optimum pH for the yeast citrate process is above 5.0. Lower pH values lead to production of polyhydroxy compounds such as erythritol and arabitol ( Tabuchi et al. , 1973 ).…”

Section: Microbial Processesmentioning

confidence: 99%

Metabolic regulation and overproduction of primary metabolites

Sánchez

Demain

2008

Microbial Biotechnology

143

103

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SummaryOverproduction of microbial metabolites is related to developmental phases of microorganisms. Inducers, effectors, inhibitors and various signal molecules play a role in different types of overproduction. Biosynthesis of enzymes catalysing metabolic reactions in microbial cells is controlled by well‐known positive and negative mechanisms, e.g. induction, nutritional regulation (carbon or nitrogen source regulation), feedback regulation, etc. The microbial production of primary metabolites contributes significantly to the quality of life. Fermentative production of these compounds is still an important goal of modern biotechnology. Through fermentation, microorganisms growing on inexpensive carbon and nitrogen sources produce valuable products such as amino acids, nucleotides, organic acids and vitamins which can be added to food to enhance its flavour, or increase its nutritive values. The contribution of microorganisms goes well beyond the food and health industries with the renewed interest in solvent fermentations. Microorganisms have the potential to provide many petroleum‐derived products as well as the ethanol necessary for liquid fuel. Additional applications of primary metabolites lie in their impact as precursors of many pharmaceutical compounds. The roles of primary metabolites and the microbes which produce them will certainly increase in importance as time goes on. In the early years of fermentation processes, development of producing strains initially depended on classical strain breeding involving repeated random mutations, each followed by screening or selection. More recently, methods of molecular genetics have been used for the overproduction of primary metabolic products. The development of modern tools of molecular biology enabled more rational approaches for strain improvement. Techniques of transcriptome, proteome and metabolome analysis, as well as metabolic flux analysis. have recently been introduced in order to identify new and important target genes and to quantify metabolic activities necessary for further strain improvement.

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“…The same condition seemed also to enhance the synthesis of a-ketoglutarate, because the condition was found to intensify the activities of NAD-and NADP-linked isocitrate dehydrogenases of the parent strain, as previously described. 9 ) In the present study, the productivity of pyruvate by the parent strain was again examined in detail from different individual n-alkanes under the same condition. Figure 3 shows that amounts of pyruvate produced from C2n+1-alkanes were nearly ten times as large as those from C 2n -alkanes, although they were only 0.7 to 0.9 mgjml.…”

Section: Productivity Of Pyruvate By the Parent Strain Of C Lipolytimentioning

confidence: 99%

A Hypothetical Cyclic Pathway for the Metabolism of Odd-carbonn-Alkanes or Propionyl-CoAviaSeven-carbon Tricarboxylic Acids in Yeasts

Tabuchi

Serizawa

1975

Agricultural and Biological Chemistry

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Preliminary Experiments on the Mechanism of Citrate Fermentation in Yeasts

Cited by 10 publications

References 0 publications

Regulation of Enzyme Synthesis of the Glyoxylate, the Citric Acid, and the Methylcitric Acid Cycles inCandida lipolytica

Regulation of Enzyme Synthesis of the Glyoxylate, the Citric Acid, and the Methylcitric Acid Cycles inCandida lipolytica

Metabolic regulation and overproduction of primary metabolites

A Hypothetical Cyclic Pathway for the Metabolism of Odd-carbonn-Alkanes or Propionyl-CoAviaSeven-carbon Tricarboxylic Acids in Yeasts

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