Preliminary experiences in trachea scaffold tissue engineering with segmental organ decellularization

Hung, Shih‐Han; Su, Cheng‐Huang; Lin, Sey‐En; Tseng, How

doi:10.1002/lary.25932

Cited by 42 publications

(59 citation statements)

References 37 publications

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“…Taking oxygen and expelling carbon dioxide is a function of the respiratory system, which is formed by multiple organs. Some of them, such as the trachea, lungs, and even diaphragm, have been tested as raw materials for decellularization purposes [133][134][135].…”

Section: Tissues Related To Respiratory Systemmentioning

confidence: 99%

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Mendibil

Ruiz-Hernández

Retegi-Carrión

et al. 2020

IJMS

185

187

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The extracellular matrix (ECM) is a complex network with multiple functions, including specific functions during tissue regeneration. Precisely, the properties of the ECM have been thoroughly used in tissue engineering and regenerative medicine research, aiming to restore the function of damaged or dysfunctional tissues. Tissue decellularization is gaining momentum as a technique to obtain potentially implantable decellularized extracellular matrix (dECM) with well-preserved key components. Interestingly, the tissue-specific dECM is becoming a feasible option to carry out regenerative medicine research, with multiple advantages compared to other approaches. This review provides an overview of the most common methods used to obtain the dECM and summarizes the strategies adopted to decellularize specific tissues, aiming to provide a helpful guide for future research development.

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Section: Tissues Related To Respiratory Systemmentioning

confidence: 99%

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Mendibil

Ruiz-Hernández

Retegi-Carrión

et al. 2020

IJMS

185

187

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“…A recent study of IBTA showed that an autologous patch grown using IBTA could serve as resorbable, constructive scaffold for the repair of partial circumference trachea in a rabbit model. 8 Regeneration of epithelium and chondrocytes was confirmed. In the present study, we examined the usefulness of BIOTUBE for the circumferential tracheal replacement.…”

Section: Discussionmentioning

confidence: 87%

“…7 The postmortem examination of the transplanted segment showed a grossly significant collapse of the tracheal tubular structure. 8 The in-body tissue architecture (IBTA), based on an encapsulation reaction, can produce autologous implantable tissue. Nakayama and colleagues reported the successful implantation of small diameter vascular grafts, "BIOTUBEs" produced by IBTA technology.…”

Section: Introductionmentioning

confidence: 99%

Tracheal Replacement Using an In-Body Tissue-Engineered Collagenous Tube “BIOTUBE” with a Biodegradable Stent in a Beagle Model: A Preliminary Report on a New Technique

Hiwatashi¹,

Nakayama

Umeda

et al. 2018

Eur J Pediatr Surg

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Introduction Tracheal reconstruction for long-segment stenosis remains challenging. We investigate the usefulness of BIOTUBE, an in-body tissue-engineered collagenous tube with a biodegradable stent, as a novel tracheal scaffold in a beagle model. Materials and Methods We prepared BIOTUBEs by embedding specially designed molds, including biodegradable stents, into subcutaneous pouches in beagles. After 2 months, the molds were filled with ingrown connective tissues and were harvested to obtain the BIOTUBEs. The BIOTUBEs, cut to 10- or 20-mm lengths, were implanted to replace the same-length defects in the cervical trachea of five beagles. Endoscopic and fluoroscopic evaluations were performed every week until the lumen became stable. The trachea, including the BIOTUBE, was harvested and subjected to histological evaluation between 3 and 7 months after implantation. Results One beagle died 28 days after 20-mm BIOTUBE implantation because of insufficient expansion and retention force of the stent. The remaining four beagles were implanted with a BIOTUBE reinforced by a strong stent, and all survived the observation period. Endoscopy revealed narrowing of the BIOTUBEs in all four beagles, due to an inflammatory reaction, but patency was maintained by steroid application at the implantation site and balloon dilatation against the stenosis. After 2 months, the lumen gradually became wider. Histological analyses showed that the internal surface of the BIOTUBEs was completely covered with tracheal epithelial cells. Conclusion This study demonstrated the usefulness of the BIOTUBE with a biodegradable stent as a novel scaffold for tracheal regeneration.

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“…The updates in creating tracheal grafts have been demonstrated through protocol improvements in physical decellularization, chemical decellularization, and bioreactor methods in the past five years. [73][74][75][76][77] These protocol advancements have also been seen in other organ systems using animal-based models such as esophagus (murine-rat), 78 lung (ovine), 79 cartilage (porcine), 80 uterus (ovine and leporine), 81,82 bladder (leporine), 83 pericardium (bovine), 84 submillimeter vasculature (murine-rat), 85 and intestine (murine-mouse) 86 model tissues. Protocol advancement in the field of vasculature has been especially important given this is often viewed as a challenge due to the size and difficulty of reseeding these grafts with endothelial cells.…”

Section: History and Development Of Decellularization For Bioenginementioning

confidence: 85%

Decellularized scaffolds for tissue engineering: Current status and future perspective

Rajab¹,

O’Malley

Tchantchaleishvili

2020

Artificial Organs

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Based on Organ Procurement and Transplantation Network data as of December 2019, more than 113 000 patients require an organ transplantation, yet, over the course of this past year, only 36 000 patients received a transplant. This discrepancy between those that need a donor organ and those that receive one remains one of medicine’s biggest challenges. Multiple solutions, both biologic and artificial, have been proposed to mitigate this difference. One of the most promising approaches to generate bioartificial organs for transplantation involves re‐seeding decellularized scaffolds with appropriate cells. Decellularization involves physical, chemical, or biological methods that typically require intimate contact of various decellularization solutions with each cell. Consequently, conventional submersion decellularization has been limited to simple tissues such as heart valves. The invention of perfusion decellularization was a breakthrough that allowed the generation of tissue‐engineered scaffolds from tissues with higher structural organization and entire organs. Such scaffolds are composed of a myriad of extracellular matrix (ECM) components that include collagen, elastin, proteoglycans, and glycoproteins. Together, these components allow the scaffolds to fulfill specialized functions, such as structural functions as well as biological functions including the regulation of cellular processes and extracellular molecules. These specialized functions of decellularized scaffolds can increasingly be harnessed for applications in tissue engineering.

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Preliminary experiences in trachea scaffold tissue engineering with segmental organ decellularization

Abstract: NA Laryngoscope, 126:2520-2527, 2016.

Cited by 42 publications

References 37 publications

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Tracheal Replacement Using an In-Body Tissue-Engineered Collagenous Tube “BIOTUBE” with a Biodegradable Stent in a Beagle Model: A Preliminary Report on a New Technique

Decellularized scaffolds for tissue engineering: Current status and future perspective

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