Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice

Abstract

Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice.

Reservoirs of Porcine Circoviruses: A Mini Review

“…Seroconversion of PCV1 antibody was detected in several murine species including NMRI, BALB/c, and Black C57 (14). Based on evidence from numerous studies, mice are often considered important animal models of PCV2 infection (26)(27)(28)(29)(30)(31)(32)(33)(34)(35). However, only a few studies have conformed PCV infection in field rodent samples.…”

Incorporation of a truncated form of flagellin (TFlg) into porcine circovirus type 2 virus-like particles enhances immune responses in mice

“…PCV2 has been shown to replicate and spread in BALB/c [24,30],and Kunming mice [32,33] .PCV2 nucleic acids can be detected in lymphoid tissues, the liver, epithelial cells, and the thymus [31]. Furthermore, the virus can be transmitted directly from mouse to mouse by contact, and it causes vertical infection through the placenta [32,[34][35][36]. Microscopic lesions in PCV2-infected mice are characterized by the expansion of germinal centers in lymphoid organs, with large numbers of histiocytic cells and lymphoblasts, apoptosis of histiocytic cells in germinal centers, and mild lymphoid depletion of the paracortex.…”

“…From a PCV2 stock (SH strain), produced as previously described and adjusted in complete DMEM to abovementioned concentrations, 50 μl were added to each well. After one hour of mixture incubation, 2*10 4 freshly tripsinised Swine Kidney (SK) cells were added to each well and incubated for 36,42 or 72 h at 37°Cin 5% CO 2 atmosphere. Then, cells were washed twice with PBS and fixed with cold absolute ethanol at − 20°C for 30 min.…”

Untitled

“…PCV2 affected raccoon dogs also show inappetence, lethargy, depression, abortion, and sterility [24,[48][49][50].…”