Preliminary evaluation of probiotic properties and safety profile of Lactiplantibacillus plantarum isolated from spontaneously fermented milk, Amabere amaruranu

Katiku, Mercy Mwikali; Matofari, Joseph Wafula; Nduko, John Masani

doi:10.1016/j.heliyon.2022.e10342

Cited by 7 publications

(6 citation statements)

References 31 publications

(63 reference statements)

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“…However, following 4 h of incubation in acidic conditions (pH 2 and 3), although the cell count in the pH 2 group was significantly lower than those in the control group and the pH 3 group (p < 0.05), no significant difference was observed between the control group and the pH 3 group (p > 0.05). Given that the digestive tract averages pH 3, these results affirm the robustness of strain cqf-43 in the acidic digestive environment [26]. Under bile salt conditions, although the cell count of strain cqf-43 was significantly lower than that of the control group (p < 0.05), it did not completely inactivate, maintaining a substantial population of viable cells (Figure 3B).…”

Section: Virulence Factorssupporting

confidence: 58%

Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis

Liu,

Zhong,

Liu

et al. 2023

IJMS

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This study reports the whole-genome sequence of Lactiplantibacillus plantarum cqf-43 isolated from healthy sow feces. Based on genomic analysis, we performed a comprehensive safety assessment of strain cqf-43, using both in vitro and in vivo experiments, and explored its probiotic potential. The total genome length measures 3,169,201 bp, boasting a GC content of 44.59%. Through phylogenetic analyses, leveraging both 16S rRNA gene and whole-genome sequences, we confidently categorize strain cqf-43 as a member of Lactiplantibacillus. Genome annotation using Prokka unveiled a total of 3141 genes, encompassing 2990 protein-coding sequences, 71 tRNAs, 16 rRNAs, and 1 tmRNA. Functional annotations derived from COG and KEGG databases highlighted a significant abundance of genes related to metabolism, with a notable emphasis on carbohydrate utilization. The genome also revealed the presence of prophage regions and CRISPR-Cas regions while lacking virulence and toxin genes. Screening for antibiotic resistance genes via the CARD database yielded no detectable transferable resistance genes, effectively eliminating the potential for harmful gene transfer. It is worth highlighting that the virulence factors identified via the VFDB database primarily contribute to bolstering pathogen resilience in hostile environments. This characteristic is particularly advantageous for probiotics. Furthermore, the genome is devoid of menacing genes such as hemolysin, gelatinase, and biogenic amine-producing genes. Our investigation also unveiled the presence of three unannotated secondary metabolite biosynthetic gene clusters, as detected by the online tool antiSMASH, suggesting a great deal of unknown potential for this strain. Rigorous in vitro experiments confirmed tolerance of strain cqf-43 in the intestinal environment, its antimicrobial efficacy, sensitivity to antibiotics, absence of hemolysis and gelatinase activity, and its inability to produce biogenic amines. In addition, a 28-day oral toxicity test showed that the strain cqf-43 did not pose a health hazard in mice, further establishing it as a safe strain.

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Section: Virulence Factorssupporting

confidence: 58%

Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis

Liu,

Zhong,

Liu

et al. 2023

IJMS

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“…Antimicrobial activity is one of the most important criteria for selecting probiotic bacteria. Previous studies approved the bio preservative applications of LAB due to the antimicrobial activities against common foodborne bacteria (Chaillou et al 2014 ; dos Santos Leandro et al 2021 ; Katiku et al 2022 ; Prabhurajeshwar et al 2017 ). In the present study, L. plantarum BG24 showed antimicrobial properties against four different pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…Susceptibility pattern was assessed using 19 different antibiotics which are Amikacin (30 µg/disc, Oxoid, England), Amoxicillin (25 µg/disc, Oxoid, England), Ampicillin (10 µg/disc, Oxoid, England), Cefamandole (30 µg/disc, Oxoid, England), Chloramphenicol (30 µg/disc, Oxoid, England), Ciprofloxacin (5 µg/disc, Oxoid, England), Doxycycline (30 µg/disc, Oxoid, England), Erythromycin (15 µg/disc, Oxoid, England), Gentamicin (10 µg/disc, Oxoid, England), Kanamycin (30 µg/disc, Oxoid, England), Lincomycin (15 µg/disc, Oxoid, England), Nalidixic acid (30 µg/disc, Oxoid, England), Penicillin (10 E/disc, Oxoid, England), Piperacillin (100 µg/disc, Oxoid, England), Oxacillin (1 µg/disc, Oxoid, England), Rifampin (5 µg/disc, Oxoid, England), Tetracycline (30 µg/disc, Oxoid, England), Tobramycin (10 µg/disc, Oxoid, England) and Vancomycin (30 µg/disc, Oxoid, England). The zone of inhibition diameters (ZOI) was measured and interpreted (Katiku et al 2022 ) as ZOI ≥ 21 mm (Sensitive, S); ZOI: 16–20 mm (Intermediately susceptible, I); ZOI ≤ 15 mm (Resistant, R).…”

Section: Methodsmentioning

confidence: 99%

A novel Lactiplantibacillus plantarum strain: probiotic properties and optimization of the growth conditions by response surface methodology

Gökmen,

Sarıyıldız,

Cholakov

et al. 2024

World J Microbiol Biotechnol

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The objective of this study is to explore the probiotic properties and optimal growth conditions of Lactiplantibacillus plantarum BG24. L. plantarum BG24 exhibited a remarkable ability to utilize lactose, and to grow under acidic conditions and in the presence of high levels of bile salts. The strain showed the highest antibacterial activity against L. monocytogenes Scott A (zone of inhibition: 26 mm). L. plantarum BG24 was found to be resistant to 8 of the tested 19 antibiotics using the disc diffusion method.and its multiple antibiotic resistance (MAR) index was calculated as 0.421. The adhesion rate to human intestinal epithelial Caco-2 cells was determined as 37.51%. The enzyme profile of L. plantarum BG24 was investigated using API ZYM test kit and the highest enzymatic activities were found for Leucine arylamidase, β-glucosidase, Valine arylamidase, β-galactosidase and N-acetyl-β-glucosaminidase. L. plantarum BG24 strain showed higher microbial growth under static conditions (6.60 OD600) compared to 100 rpm (5.73 OD600) and 200 rpm (5.02 OD600) shaking speed due to its facultative anaerobic characteristic. However, different inoculation rates and glucose addition did not make a statistically significant difference on biomass formation (p > 0.05). The specific growth rate of L. plantarum BG24 was 0.416 h−1, the doubling time was 1.67 h, and the biomass productivity value was 0.14 gL−1 h−1 in the original MRS broth (pH 5.7) while higher values were found as 0.483 h−1, 1.43 h and 0.17 gL−1 h−1, respectively, in MRS broth (pH 6.5) medium enriched with 5 g/L yeast extract. The stirred tank bioreactor was used to optimise the growth of BG24 strain. The process variables was optimized at 0.05 vvm of aeration rate, 479 rpm of agitation speed, 3% of inoculation rate and 18 h of incubation time. The maximum biomass (g/L) production was obtained as 3.84 g/L at the optimized conditions.

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“…The plates were then incubated at 37°C for 24 h. The diameters of the inhibition zones around each disk (ZDI values) were measured and interpreted according to the Clinical and Laboratory Standards Institute (CLSI) 2009 guidelines. The results were categorized as follows: resistant (ZDI: ≤ 15 mm), sensitive (ZDI: ≥ 21 mm), or intermediately susceptible (ZDI: 16–20 mm) ( Katiku et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the probiotic, technological, safety attributes, and GABA-producing capacity of microorganisms isolated from Iranian milk kefir beverages

Moghimani,

Onyeaka,

Hashemi

et al. 2024

Front. Microbiol.

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IntroductionKefir beverage has beneficial microorganisms that have health-giving properties; therefore, they have a good potential to be probiotic. This study evaluated the probiotic potential, technological, and safety characteristics of Enterococcus faecalis, Lactococcus lactis, and Pichia fermentans isolated from traditional kefir beverages.MethodFirst, isolates were evaluated in terms of resistance to acid, alkali, bile salts, trypsin, and pepsin of the gastrointestinal tract. The auto-aggregation and co-aggregation ability of isolates were measured using spectrophotometry. Antimicrobial activities were assayed against important food-borne pathogens using the agar well diffusion method. Moreover, gamma-aminobutyric acid (GABA) production was investigated by thin-layer chromatography (TLC).ResultAmong the isolates, P. fermentans had an 85% total survival rate, but its amount reached below 6 log CFU/ml which is considered non-resistant, and it showed the highest auto-aggregation (74.67%). Moreover, only L. lactis showed antimicrobial activity and had the highest co-aggregation with E. coli PTCC 1338 (54.33%) and L. monocytogenes ATCC 7644 (78%). Finally, an evaluation of the technological and safety characteristics of the strains showed that the strains produced GABA and were safe.DiscussionAlthough the isolates were not resistant to the gastrointestinal tract, their supernatant contained valuable natural compounds, including antioxidants, GABA, and antimicrobials, which can be used to produce functional foods and medicines. In addition, other approaches, such as increasing the initial number of strains, using foods as carriers of isolates, and encapsulating the isolates, can effectively increase the survivability of isolates in the gastrointestinal tract.

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Preliminary evaluation of probiotic properties and safety profile of Lactiplantibacillus plantarum isolated from spontaneously fermented milk, Amabere amaruranu

Cited by 7 publications

References 31 publications

Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis

Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis

A novel Lactiplantibacillus plantarum strain: probiotic properties and optimization of the growth conditions by response surface methodology

Evaluation of the probiotic, technological, safety attributes, and GABA-producing capacity of microorganisms isolated from Iranian milk kefir beverages

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