Preliminary evaluation of a rapid colorimetric method for identification of pathogenic Neisseria

Hosmer, Marion E.; Cohenford, Menashi A.; Ellner, Paul D.

doi:10.1128/jcm.24.1.141-142.1986

Cited by 14 publications

(7 citation statements)

References 5 publications

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“…The production of hydroxyprolylaminopeptidase by N. cinerea, but not by B. catarrhalis, is of possible differential value (147,177). This enzyme was produced by all N. gonorrhoeae strains examined (84,147,150) and by various percentages of N. meningitidis (84,147,177).…”

Section: Identification and Biochemical Characteristics Of B Catarrhmentioning

confidence: 94%

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Branhamella catarrhalis: an organism gaining respect as a pathogen.

Catlin

1990

Clinical Microbiology Reviews

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Section: Identification and Biochemical Characteristics Of B Catarrhmentioning

confidence: 94%

“…Various commercial enzyme substrate kits for rapid tests have been described (94,150,155,177,212,233,315). Unfortunately, some rapid tests yield only negative reactions for B. catarrhalis or, worse, false identifications (40,94,109,233).…”

Section: Stages In the Identification Processmentioning

confidence: 99%

Branhamella catarrhalis: an organism gaining respect as a pathogen.

Catlin

1990

Clinical Microbiology Reviews

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“…meningitidis, five N. lac tamica, four Mor. catarrhalis) Hosmer et al (1986) reported loo'% correlation between the Neisseria-Identicult, fermentation tests on Columbia agar slants and the Phadebact Monoclonal GC test. Sperry et al (1986) found 97% correlation between the Neisseria-Identicult and MNYC from a total of 104 isolates (54 N .…”

Section: Discussionmentioning

confidence: 99%

Rapid identification of pathogenic neisserias using the Identicult-Neisseria test

Moyes

Chronas

Young³

1994

Lett Appl Microbiol

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A rapid enzymatic method using chromogenic substrates for the rapid identification of pathogenic neisseria (Identicult-Neisseria, Scott Laboratories Inc., CA, USA) was tested in parallel with the rapid carbohydrate utilization test (RCUT) and the Phadebact Monoclonal GC Test against 198 consecutive clinical isolates of oxidase-positive Gram-negative diplococci (118 Neisseria gonorrhoeae, 76 N. meningitidis and four N. lactamica). On initial testing the Identicult-Neisseria gave a 95% overall concordance (97.5% N. gonorrhoeae, 90.8% N. meningitidis) with the RCUT and Phadebact tests; the corresponding figures after repeat testing were 98% overall concordance (98.3% N. gonorrhoeae, 97.4% N. meningitidis). Two of the three strains of N. gonorrhoeae mis-identified as N. meningitidis on primary testing were also mis-identified on repeat testing. Seven strains of N. meningitidis were mis-identified on initial testing (six as Moraxella catarrhalis and one as N. lactamica) and two on repeat testing (both as Mor. catarrhalis). We conclude that the Identicult-Neisseria is not sufficiently reliable for the culture confirmation of gonococci and meningococci.

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“…Methods for the identification of Neisseria spp. include carbohydrate degradation tests (8,13), chromogenic enzyme substrate tests (6,7,16,17), and combinations of these two approaches (14,15). Conventional procedures use cystinetryptic digest agar (CTA) base medium with added carbohydrates and may require prolonged incubation for some isolates before results are available (13).…”

mentioning

confidence: 99%

“…Most recently, identification methods with specific chromogenic enzyme substrates have become available for rapid identification of Neisseria spp. that grow on selective medium (i.e., N. gonorrhoeae, N. meningitidis, and N. lactamica) (6,7,14,16,17). These systems also provide a presumptive identification of Branhamella catarrhalis, an organism of recognized clinical importance that can also be occasionally recovered on selective medium (e.g., modified Thayer-Martin [MTMI and Martin-Lewis agars) (4; G. V. Doern, Clin.…”

mentioning

confidence: 99%

API QuadFERM+ with rapid DNase for identification of Neisseria spp. and Branhamella catarrhalis

Janda¹,

Zigler²,

Bradna³

1987

J Clin Microbiol

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The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The two misidentified meningococcal strains were biochemically atypical and were also misidentified by the conventional method. Of 26 N. lactamica strains, 25 (96%) were correctly identified. Of 21 Neisseria spp., 14 (67%) produced carbohydrate reactions in agreement with the conventional procedure, and 7 strains produced detectable acid in the QuadFERM + from maltose and sucrose but not glucose. All 9 N. cinerea and 30 B. catarrhalis strains were asaccharolytic by QuadFERM+. The rapid DNase test was positive for all B. catarrhalis strains and negative for all other organisms. Two beta-lactamase-positive N. gonorrhoeae strains and 25 (93%) of 27 beta-lactamase-positive B. catarrhalis strains were detected by the 2-h acidometric beta-lactamase test on the strip. QuadFERM+ with rapid DNase is a simple and easily interpretable method for identification of these organisms in the clinical laboratory.

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Preliminary evaluation of a rapid colorimetric method for identification of pathogenic Neisseria

Cited by 14 publications

References 5 publications

Branhamella catarrhalis: an organism gaining respect as a pathogen.

Branhamella catarrhalis: an organism gaining respect as a pathogen.

Rapid identification of pathogenic neisserias using the Identicult-Neisseria test

API QuadFERM+ with rapid DNase for identification of Neisseria spp. and Branhamella catarrhalis

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